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Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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Response surface plots (a) and contour plots (b) for the fucoidanase activity optimization. The interaction between kelp powder and wheat bran.
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marinedrugs-13-06818-f003: Response surface plots (a) and contour plots (b) for the fucoidanase activity optimization. The interaction between kelp powder and wheat bran.

Mentions: Three dimensional response surface plots (Figure 3a) and 2D contour plots (Figure 3b) of fucoidanase production graphically representing regression equations were used to demonstrate relationships between the response and experimental levels of each variable. Each figure presents the effect of two factors, whereas the other factor was held at zero level. As shown in the surface plots, there was an interaction between each pair of variables. All the interaction between the selected three variables was significant.


Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Response surface plots (a) and contour plots (b) for the fucoidanase activity optimization. The interaction between kelp powder and wheat bran.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f003: Response surface plots (a) and contour plots (b) for the fucoidanase activity optimization. The interaction between kelp powder and wheat bran.
Mentions: Three dimensional response surface plots (Figure 3a) and 2D contour plots (Figure 3b) of fucoidanase production graphically representing regression equations were used to demonstrate relationships between the response and experimental levels of each variable. Each figure presents the effect of two factors, whereas the other factor was held at zero level. As shown in the surface plots, there was an interaction between each pair of variables. All the interaction between the selected three variables was significant.

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

Show MeSH