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Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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Growth and fucoidanase activity of Streptomyces sp.
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marinedrugs-13-06818-f002: Growth and fucoidanase activity of Streptomyces sp.

Mentions: The correlation between fucoidanase production and culturing time may differ among different organisms [9]. It was observed that an extension in cultivation period resulted in an increase in cell growth with a concomitant increase in fucoidanase activity (Figure 2). The production of the fucoidanase was associated with cell growth, increased during the logarithmic growth, and reached its maximum fucoidanase activity in the stationary phase (96 h). Similar to our findings, fucoidanase production by both Dendryphiella arenaria TM94 [9] and Pseudoalteromonas citrea [11] was synchronous with cell growth and reached the maximum concentration in the stationary phase of the cell.


Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Growth and fucoidanase activity of Streptomyces sp.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f002: Growth and fucoidanase activity of Streptomyces sp.
Mentions: The correlation between fucoidanase production and culturing time may differ among different organisms [9]. It was observed that an extension in cultivation period resulted in an increase in cell growth with a concomitant increase in fucoidanase activity (Figure 2). The production of the fucoidanase was associated with cell growth, increased during the logarithmic growth, and reached its maximum fucoidanase activity in the stationary phase (96 h). Similar to our findings, fucoidanase production by both Dendryphiella arenaria TM94 [9] and Pseudoalteromonas citrea [11] was synchronous with cell growth and reached the maximum concentration in the stationary phase of the cell.

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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