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Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

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Phylogenetic tree of 10 strains of scab-causing and related Streptomyces sp. based on the 16S rDNA gene sequence. The numbers at the branching points are the percentages of occurrence in 1000 bootstrapped trees. The bar indicates a distance of 0.02 substitutions per site.
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marinedrugs-13-06818-f001: Phylogenetic tree of 10 strains of scab-causing and related Streptomyces sp. based on the 16S rDNA gene sequence. The numbers at the branching points are the percentages of occurrence in 1000 bootstrapped trees. The bar indicates a distance of 0.02 substitutions per site.

Mentions: The marine actinobacterium Streptomyces sp. was isolated from the marine sediment samples collected from the Busan coast, South Korea, and was used for the production of fucoidanase. On the basis of the growth rate and high fucoidanase activity, Streptomyces sp. was considered as an effective producer of fucoidanase. The active producer was identified using cultural, morphological, biochemical, physiological characteristics, and 16S rDNA sequence (Figure 1). The isolate was identified as Streptomyces sp., and the 16S rDNA sequence of actinobacterium Streptomyces was deposited in NCBI (Accession No. KC179795).


Production of a Novel Fucoidanase for the Green Synthesis of Gold Nanoparticles by Streptomyces sp. and Its Cytotoxic Effect on HeLa Cells.

Manivasagan P, Oh J - Mar Drugs (2015)

Phylogenetic tree of 10 strains of scab-causing and related Streptomyces sp. based on the 16S rDNA gene sequence. The numbers at the branching points are the percentages of occurrence in 1000 bootstrapped trees. The bar indicates a distance of 0.02 substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663555&req=5

marinedrugs-13-06818-f001: Phylogenetic tree of 10 strains of scab-causing and related Streptomyces sp. based on the 16S rDNA gene sequence. The numbers at the branching points are the percentages of occurrence in 1000 bootstrapped trees. The bar indicates a distance of 0.02 substitutions per site.
Mentions: The marine actinobacterium Streptomyces sp. was isolated from the marine sediment samples collected from the Busan coast, South Korea, and was used for the production of fucoidanase. On the basis of the growth rate and high fucoidanase activity, Streptomyces sp. was considered as an effective producer of fucoidanase. The active producer was identified using cultural, morphological, biochemical, physiological characteristics, and 16S rDNA sequence (Figure 1). The isolate was identified as Streptomyces sp., and the 16S rDNA sequence of actinobacterium Streptomyces was deposited in NCBI (Accession No. KC179795).

Bottom Line: The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively.Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h.Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

View Article: PubMed Central - PubMed

Affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Korea. manimaribtech@gmail.com.

ABSTRACT
Marine actinobacteria-produced fucoidanases have received considerable attention as one of the major research topics in recent years, particularly for the medical exploitation of fucoidans and their degradation products. The present study describes the optimization and production of a novel fucoidanase for the green synthesis of gold nanoparticles and its biological applications. The production of fucoidanase was optimized using Streptomyces sp. The medium components were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodology. The fucoidanase was statistically optimized with the most significant factors, namely wheat bran 3.3441 g/L, kelp powder 0.7041 g/L, and NaCl 0.8807 g/L, respectively. The biosynthesized gold nanoparticles were determined by UV-vis spectroscopy and were further characterized by X-ray diffraction analysis, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, energy dispersive X-ray analysis, and high-resolution transmission electron microscopy. Furthermore, the biosynthesized gold nanoparticles exhibited a dose-dependent cytotoxicity against HeLa cells and the inhibitory concentration (IC50) was found to be 350 µg/mL at 24 h and 250 µg/mL at 48 h. Therefore, the production of novel fucoidanase for the green synthesis of gold nanoparticles has comparatively rapid, less expensive and wide application to anticancer therapy in modern medicine.

Show MeSH