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Seasonal Changes in Mycosporine-Like Amino Acid Production Rate with Respect to Natural Phytoplankton Species Composition.

Ha SY, Lee Y, Kim MS, Kumar KS, Shin KH - Mar Drugs (2015)

Bottom Line: The dominance of diatoms indicated that they had a long-term response to UVR.Our results demonstrate that both the net production rate as well as the concentration of MAAs related to photoinduction depended on the phytoplankton community structure.In addition, seasonal changes in UVR also influenced the quantity and production of MAAs in phytoplanktons (especially Cyanophyceae).

View Article: PubMed Central - PubMed

Affiliation: Division of Polar Ocean Environment, Korea Polar Research Institute (KOPRI), 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Korea. syha@kopri.re.kr.

ABSTRACT
After in situ incubation at the site for a year, phytoplanktons in surface water were exposed to natural light in temperate lakes (every month); thereafter, the net production rate of photoprotective compounds (mycosporine-like amino acids, MAAs) was calculated using (13)C labeled tracer. This is the first report describing seasonal variation in the net production rate of individual MAAs in temperate lakes using a compound-specific stable isotope method. In the mid-latitude region of the Korean Peninsula, UV radiation (UVR) usually peaks from July to August. In Lake Paldang and Lake Cheongpyeong, diatoms dominated among the phytoplankton throughout the year. The relative abundance of Cyanophyceae (Anabaena spiroides) reached over 80% during July in Lake Cheongpyeong. Changes in phytoplankton abundance indicate that the phytoplankton community structure is influenced by seasonal changes in the net production rate and concentration of MAAs. Notably, particulate organic matter (POM) showed a remarkable change based on the UV intensity occurring during that period; this was because of the fact that cyanobacteria that are highly sensitive to UV irradiance dominated the community. POM cultured in Lake Paldang had the greatest shinorine (SH) production rate during October, i.e., 83.83 ± 10.47 fgC·L(-1)·h(-1). The dominance of diatoms indicated that they had a long-term response to UVR. Evaluation of POM cultured in Lake Cheongpyeong revealed that there was an increase in the net MAA production in July (when UVR reached the maximum); a substantial amount of SH, i.e., 17.62 ± 18.34 fgC·L(-1)·h(-1), was recorded during this period. Our results demonstrate that both the net production rate as well as the concentration of MAAs related to photoinduction depended on the phytoplankton community structure. In addition, seasonal changes in UVR also influenced the quantity and production of MAAs in phytoplanktons (especially Cyanophyceae).

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Seasonal change in natural concentration of individual MAAs in Lake Paldang (a) and Cheongpyeong (b). SH: Shinorine (black); PA: Palythine (gray); MG: Mycosporine-glycine (dark gray).
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marinedrugs-13-06740-f001: Seasonal change in natural concentration of individual MAAs in Lake Paldang (a) and Cheongpyeong (b). SH: Shinorine (black); PA: Palythine (gray); MG: Mycosporine-glycine (dark gray).

Mentions: In Lake Paldang, relatively high MAA concentrations, i.e., 8.56 ± 0.09 ng/μg chl a, were observed in October. In September and November, MAA concentrations of 3.35 ± 0.21 ng/μg chl a and 3.38 ± 0.12 ng/μg chl a, were respectively observed. On the other hand, the MAA concentration was 0.02 ± 0.002 ng/μg chl a to 0.61 ± 0.17 ng/μg chl a from March to August. During May, no MAA concentration was detected on some occasions. Amongst the MAA, the MG and PA were high every month, while the SH were detected at relatively low concentrations (Figure 1).


Seasonal Changes in Mycosporine-Like Amino Acid Production Rate with Respect to Natural Phytoplankton Species Composition.

Ha SY, Lee Y, Kim MS, Kumar KS, Shin KH - Mar Drugs (2015)

Seasonal change in natural concentration of individual MAAs in Lake Paldang (a) and Cheongpyeong (b). SH: Shinorine (black); PA: Palythine (gray); MG: Mycosporine-glycine (dark gray).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663551&req=5

marinedrugs-13-06740-f001: Seasonal change in natural concentration of individual MAAs in Lake Paldang (a) and Cheongpyeong (b). SH: Shinorine (black); PA: Palythine (gray); MG: Mycosporine-glycine (dark gray).
Mentions: In Lake Paldang, relatively high MAA concentrations, i.e., 8.56 ± 0.09 ng/μg chl a, were observed in October. In September and November, MAA concentrations of 3.35 ± 0.21 ng/μg chl a and 3.38 ± 0.12 ng/μg chl a, were respectively observed. On the other hand, the MAA concentration was 0.02 ± 0.002 ng/μg chl a to 0.61 ± 0.17 ng/μg chl a from March to August. During May, no MAA concentration was detected on some occasions. Amongst the MAA, the MG and PA were high every month, while the SH were detected at relatively low concentrations (Figure 1).

Bottom Line: The dominance of diatoms indicated that they had a long-term response to UVR.Our results demonstrate that both the net production rate as well as the concentration of MAAs related to photoinduction depended on the phytoplankton community structure.In addition, seasonal changes in UVR also influenced the quantity and production of MAAs in phytoplanktons (especially Cyanophyceae).

View Article: PubMed Central - PubMed

Affiliation: Division of Polar Ocean Environment, Korea Polar Research Institute (KOPRI), 26 Songdomirae-ro, Yeonsu-gu, Incheon 406-840, Korea. syha@kopri.re.kr.

ABSTRACT
After in situ incubation at the site for a year, phytoplanktons in surface water were exposed to natural light in temperate lakes (every month); thereafter, the net production rate of photoprotective compounds (mycosporine-like amino acids, MAAs) was calculated using (13)C labeled tracer. This is the first report describing seasonal variation in the net production rate of individual MAAs in temperate lakes using a compound-specific stable isotope method. In the mid-latitude region of the Korean Peninsula, UV radiation (UVR) usually peaks from July to August. In Lake Paldang and Lake Cheongpyeong, diatoms dominated among the phytoplankton throughout the year. The relative abundance of Cyanophyceae (Anabaena spiroides) reached over 80% during July in Lake Cheongpyeong. Changes in phytoplankton abundance indicate that the phytoplankton community structure is influenced by seasonal changes in the net production rate and concentration of MAAs. Notably, particulate organic matter (POM) showed a remarkable change based on the UV intensity occurring during that period; this was because of the fact that cyanobacteria that are highly sensitive to UV irradiance dominated the community. POM cultured in Lake Paldang had the greatest shinorine (SH) production rate during October, i.e., 83.83 ± 10.47 fgC·L(-1)·h(-1). The dominance of diatoms indicated that they had a long-term response to UVR. Evaluation of POM cultured in Lake Cheongpyeong revealed that there was an increase in the net MAA production in July (when UVR reached the maximum); a substantial amount of SH, i.e., 17.62 ± 18.34 fgC·L(-1)·h(-1), was recorded during this period. Our results demonstrate that both the net production rate as well as the concentration of MAAs related to photoinduction depended on the phytoplankton community structure. In addition, seasonal changes in UVR also influenced the quantity and production of MAAs in phytoplanktons (especially Cyanophyceae).

Show MeSH