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Effects of Cylindrospermopsin Producing Cyanobacterium and Its Crude Extracts on a Benthic Green Alga-Competition or Allelopathy?

B-Béres V, Vasas G, Dobronoki D, Gonda S, Nagy SA, Bácsi I - Mar Drugs (2015)

Bottom Line: The growth inhibition of Chlorococcum sp. increased with the increasing ratio of the cyanobacterium in mixed cultures (inhibition ranged from 26% to 87% compared to control).Interestingly, inhibition of the cyanobacterium growth also occurred in mixed cultures, and it was more pronounced than it was expected.The inhibitory effects of cyanobacterial crude extracts on Chlorococcum cultures were concentration-dependent.

View Article: PubMed Central - PubMed

Affiliation: Environmental Laboratory, Department of Environment and Conservation, Hajdú-Bihar County Government Office, 16 Hatvan street, Debrecen H-4025, Hungary. beres.viktoria@gmail.com.

ABSTRACT
Cylindrospermopsin (CYN) is a toxic secondary metabolite produced by filamentous cyanobacteria which could work as an allelopathic substance, although its ecological role in cyanobacterial-algal assemblages is mostly unclear. The competition between the CYN-producing cyanobacterium Chrysosporum (Aphanizomenon) ovalisporum, and the benthic green alga Chlorococcum sp. was investigated in mixed cultures, and the effects of CYN-containing cyanobacterial crude extract on Chlorococcum sp. were tested by treatments with crude extracts containing total cell debris, and with cell debris free crude extracts, modelling the collapse of a cyanobacterial water bloom. The growth inhibition of Chlorococcum sp. increased with the increasing ratio of the cyanobacterium in mixed cultures (inhibition ranged from 26% to 87% compared to control). Interestingly, inhibition of the cyanobacterium growth also occurred in mixed cultures, and it was more pronounced than it was expected. The inhibitory effects of cyanobacterial crude extracts on Chlorococcum cultures were concentration-dependent. The presence of C. ovalisporum in mixed cultures did not cause significant differences in nutrient content compared to Chlorococcum control culture, so the growth inhibition of the green alga could be linked to the presence of CYN and/or other bioactive compounds.

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Changes of cell number in C. ovalisporum crude extract treated Chlorococcum cultures. (a) Cultures treated with CDC crude extract; and (b) cultures treated with CDF crude extract. Significant differences are indicated with different lowercase letters.
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marinedrugs-13-06703-f005: Changes of cell number in C. ovalisporum crude extract treated Chlorococcum cultures. (a) Cultures treated with CDC crude extract; and (b) cultures treated with CDF crude extract. Significant differences are indicated with different lowercase letters.

Mentions: The increase of cell number was significantly lower in the CDC crude extract treated cultures only in the case of 1:4 ratio (p < 0.05; Figure 5a). Changes of cell number of the green algal cultures treated with different amount of CDC crude extract differed from each other significantly, except of 1:1 and 1:2 cultures (p < 0.05; Figure 5a). The inhibition of growth was clear in culture treated with CDC crude extract in 1:2 ratio from the seventh day, cell number of this CDC crude extract treated culture was 10.5% lower than control culture on the last day of the experiment (Figure 5a). The most conspicuous inhibition was detected in culture treated with CDC crude extract in 1:4 ratio, cell number of this treated culture was 27% lower than in control culture on the 14th day (Figure 5a).


Effects of Cylindrospermopsin Producing Cyanobacterium and Its Crude Extracts on a Benthic Green Alga-Competition or Allelopathy?

B-Béres V, Vasas G, Dobronoki D, Gonda S, Nagy SA, Bácsi I - Mar Drugs (2015)

Changes of cell number in C. ovalisporum crude extract treated Chlorococcum cultures. (a) Cultures treated with CDC crude extract; and (b) cultures treated with CDF crude extract. Significant differences are indicated with different lowercase letters.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663549&req=5

marinedrugs-13-06703-f005: Changes of cell number in C. ovalisporum crude extract treated Chlorococcum cultures. (a) Cultures treated with CDC crude extract; and (b) cultures treated with CDF crude extract. Significant differences are indicated with different lowercase letters.
Mentions: The increase of cell number was significantly lower in the CDC crude extract treated cultures only in the case of 1:4 ratio (p < 0.05; Figure 5a). Changes of cell number of the green algal cultures treated with different amount of CDC crude extract differed from each other significantly, except of 1:1 and 1:2 cultures (p < 0.05; Figure 5a). The inhibition of growth was clear in culture treated with CDC crude extract in 1:2 ratio from the seventh day, cell number of this CDC crude extract treated culture was 10.5% lower than control culture on the last day of the experiment (Figure 5a). The most conspicuous inhibition was detected in culture treated with CDC crude extract in 1:4 ratio, cell number of this treated culture was 27% lower than in control culture on the 14th day (Figure 5a).

Bottom Line: The growth inhibition of Chlorococcum sp. increased with the increasing ratio of the cyanobacterium in mixed cultures (inhibition ranged from 26% to 87% compared to control).Interestingly, inhibition of the cyanobacterium growth also occurred in mixed cultures, and it was more pronounced than it was expected.The inhibitory effects of cyanobacterial crude extracts on Chlorococcum cultures were concentration-dependent.

View Article: PubMed Central - PubMed

Affiliation: Environmental Laboratory, Department of Environment and Conservation, Hajdú-Bihar County Government Office, 16 Hatvan street, Debrecen H-4025, Hungary. beres.viktoria@gmail.com.

ABSTRACT
Cylindrospermopsin (CYN) is a toxic secondary metabolite produced by filamentous cyanobacteria which could work as an allelopathic substance, although its ecological role in cyanobacterial-algal assemblages is mostly unclear. The competition between the CYN-producing cyanobacterium Chrysosporum (Aphanizomenon) ovalisporum, and the benthic green alga Chlorococcum sp. was investigated in mixed cultures, and the effects of CYN-containing cyanobacterial crude extract on Chlorococcum sp. were tested by treatments with crude extracts containing total cell debris, and with cell debris free crude extracts, modelling the collapse of a cyanobacterial water bloom. The growth inhibition of Chlorococcum sp. increased with the increasing ratio of the cyanobacterium in mixed cultures (inhibition ranged from 26% to 87% compared to control). Interestingly, inhibition of the cyanobacterium growth also occurred in mixed cultures, and it was more pronounced than it was expected. The inhibitory effects of cyanobacterial crude extracts on Chlorococcum cultures were concentration-dependent. The presence of C. ovalisporum in mixed cultures did not cause significant differences in nutrient content compared to Chlorococcum control culture, so the growth inhibition of the green alga could be linked to the presence of CYN and/or other bioactive compounds.

Show MeSH
Related in: MedlinePlus