Limits...
Botulinum toxin type A induces changes in the chemical coding of substance P-immunoreactive dorsal root ganglia sensory neurons supplying the porcine urinary bladder.

Bossowska A, Lepiarczyk E, Mazur U, Janikiewicz P, Markiewicz W - Toxins (Basel) (2015)

Bottom Line: Nearly half of these neurons co-expressed PACAP or CGRP (45% and 35%, respectively), while co-localization of SP with GAL, nNOS, SOM or CB was found less frequently (3.7%, 1.8%, 1.2%, and 0.7%, respectively).In BTX-treated pigs (n = 6), toxin-injections caused a decrease in the number of SP-IR cells containing CGRP, SOM or CB (16.2%, 0.5%, and 0%, respectively) and a distinct increase in these nerve cells immunopositive to GAL (27.2%).The present study demonstrates that BTX significantly modifies the chemical phenotypes of SP-IR BPSN.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Warszawska 30, Olsztyn 10-082, Poland. agnieszka.bossowska@uwm.edu.pl.

ABSTRACT
Botulinum toxin (BTX) is a potent neurotoxin which blocks acetylcholine release from nerve terminals, and therefore leads to cessation of somatic motor and/or parasympathetic transmission. Recently it has been found that BTX also interferes with sensory transmission, thus, the present study was aimed at investigating the neurochemical characterization of substance P-immunoreactive (SP-IR) bladder-projecting sensory neurons (BPSN) after the toxin treatment. Investigated neurons were visualized with retrograde tracing method and their chemical profile was disclosed with double-labelling immunohistochemistry using antibodies against SP, calcitonin gene-related peptide (CGRP), pituitary adenylate cyclase activating polypeptide (PACAP), neuronal nitric oxide synthase (nNOS), galanin (GAL), calbindin (CB), and somatostatin (SOM). In the control group (n = 6), 45% of the total population of BPSN were SP-IR. Nearly half of these neurons co-expressed PACAP or CGRP (45% and 35%, respectively), while co-localization of SP with GAL, nNOS, SOM or CB was found less frequently (3.7%, 1.8%, 1.2%, and 0.7%, respectively). In BTX-treated pigs (n = 6), toxin-injections caused a decrease in the number of SP-IR cells containing CGRP, SOM or CB (16.2%, 0.5%, and 0%, respectively) and a distinct increase in these nerve cells immunopositive to GAL (27.2%). The present study demonstrates that BTX significantly modifies the chemical phenotypes of SP-IR BPSN.

Show MeSH

Related in: MedlinePlus

A schematic diagram of a spinal ganglion section showing its arbitrary division into topographical domains, in which the occurrence and relative frequency of substance P (SP) containing bladder projecting sensory neurons (BPSN) were studied. Presented data were pooled from all dorsal root ganglia (DRG) sections studied, both the ipsi- and contralateral ganglia. C—central, P—peripheral, Cr—cranial and Cd—caudal domains of the DRG, M—middle region of the ganglion.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4663534&req=5

toxins-07-04797-f003: A schematic diagram of a spinal ganglion section showing its arbitrary division into topographical domains, in which the occurrence and relative frequency of substance P (SP) containing bladder projecting sensory neurons (BPSN) were studied. Presented data were pooled from all dorsal root ganglia (DRG) sections studied, both the ipsi- and contralateral ganglia. C—central, P—peripheral, Cr—cranial and Cd—caudal domains of the DRG, M—middle region of the ganglion.


Botulinum toxin type A induces changes in the chemical coding of substance P-immunoreactive dorsal root ganglia sensory neurons supplying the porcine urinary bladder.

Bossowska A, Lepiarczyk E, Mazur U, Janikiewicz P, Markiewicz W - Toxins (Basel) (2015)

A schematic diagram of a spinal ganglion section showing its arbitrary division into topographical domains, in which the occurrence and relative frequency of substance P (SP) containing bladder projecting sensory neurons (BPSN) were studied. Presented data were pooled from all dorsal root ganglia (DRG) sections studied, both the ipsi- and contralateral ganglia. C—central, P—peripheral, Cr—cranial and Cd—caudal domains of the DRG, M—middle region of the ganglion.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663534&req=5

toxins-07-04797-f003: A schematic diagram of a spinal ganglion section showing its arbitrary division into topographical domains, in which the occurrence and relative frequency of substance P (SP) containing bladder projecting sensory neurons (BPSN) were studied. Presented data were pooled from all dorsal root ganglia (DRG) sections studied, both the ipsi- and contralateral ganglia. C—central, P—peripheral, Cr—cranial and Cd—caudal domains of the DRG, M—middle region of the ganglion.
Bottom Line: Nearly half of these neurons co-expressed PACAP or CGRP (45% and 35%, respectively), while co-localization of SP with GAL, nNOS, SOM or CB was found less frequently (3.7%, 1.8%, 1.2%, and 0.7%, respectively).In BTX-treated pigs (n = 6), toxin-injections caused a decrease in the number of SP-IR cells containing CGRP, SOM or CB (16.2%, 0.5%, and 0%, respectively) and a distinct increase in these nerve cells immunopositive to GAL (27.2%).The present study demonstrates that BTX significantly modifies the chemical phenotypes of SP-IR BPSN.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Warszawska 30, Olsztyn 10-082, Poland. agnieszka.bossowska@uwm.edu.pl.

ABSTRACT
Botulinum toxin (BTX) is a potent neurotoxin which blocks acetylcholine release from nerve terminals, and therefore leads to cessation of somatic motor and/or parasympathetic transmission. Recently it has been found that BTX also interferes with sensory transmission, thus, the present study was aimed at investigating the neurochemical characterization of substance P-immunoreactive (SP-IR) bladder-projecting sensory neurons (BPSN) after the toxin treatment. Investigated neurons were visualized with retrograde tracing method and their chemical profile was disclosed with double-labelling immunohistochemistry using antibodies against SP, calcitonin gene-related peptide (CGRP), pituitary adenylate cyclase activating polypeptide (PACAP), neuronal nitric oxide synthase (nNOS), galanin (GAL), calbindin (CB), and somatostatin (SOM). In the control group (n = 6), 45% of the total population of BPSN were SP-IR. Nearly half of these neurons co-expressed PACAP or CGRP (45% and 35%, respectively), while co-localization of SP with GAL, nNOS, SOM or CB was found less frequently (3.7%, 1.8%, 1.2%, and 0.7%, respectively). In BTX-treated pigs (n = 6), toxin-injections caused a decrease in the number of SP-IR cells containing CGRP, SOM or CB (16.2%, 0.5%, and 0%, respectively) and a distinct increase in these nerve cells immunopositive to GAL (27.2%). The present study demonstrates that BTX significantly modifies the chemical phenotypes of SP-IR BPSN.

Show MeSH
Related in: MedlinePlus