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Metabolic and hematological consequences of dietary deoxynivalenol interacting with systemic Escherichia coli lipopolysaccharide.

Bannert E, Tesch T, Kluess J, Frahm J, Kersten S, Kahlert S, Renner L, Rothkötter HJ, Dänicke S - Toxins (Basel) (2015)

Bottom Line: DON-feeding solely decreased portal glucose uptake (p < 0.05).LPS-decreased partial oxygen pressure (pO₂) overall (p < 0.05), but reduced pCO₂ only in arterial blood, and DON had no effect on either.Irrespective of catheter localization, LPS decreased pH and base-excess (p < 0.01), but increased lactate and anion-gap (p < 0.01), indicating an emerging lactic acidosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, Braunschweig 38116, Germany. Erik.Bannert@fli.bund.de.

ABSTRACT
Previous studies have shown that chronic oral deoxynivalenol (DON) exposure modulated Escherichia coli lipopolysaccharide (LPS)-induced systemic inflammation, whereby the liver was suspected to play an important role. Thus, a total of 41 barrows was fed one of two maize-based diets, either a DON-diet (4.59 mg DON/kg feed, n = 19) or a control diet (CON, n = 22). Pigs were equipped with indwelling catheters for pre- or post-hepatic (portal vs. jugular catheter) infusion of either control (0.9% NaCl) or LPS (7.5 µg/kg BW) for 1h and frequent blood sampling. This design yielded six groups: CON_CONjugular‑CONportal, CON_CONjugular‑LPSportal, CON_LPSjugular‑CONportal, DON_CONjugular‑CONportal, DON_CONjugular‑LPSportal and DON_LPSjugular‑CONportal. Blood samples were analyzed for blood gases, electrolytes, glucose, pH, lactate and red hemogram. The red hemogram and electrolytes were not affected by DON and LPS. DON-feeding solely decreased portal glucose uptake (p < 0.05). LPS-decreased partial oxygen pressure (pO₂) overall (p < 0.05), but reduced pCO₂ only in arterial blood, and DON had no effect on either. Irrespective of catheter localization, LPS decreased pH and base-excess (p < 0.01), but increased lactate and anion-gap (p < 0.01), indicating an emerging lactic acidosis. Lactic acidosis was more pronounced in the group DON_LPSjugular-CONportal than in CON-fed counterparts (p < 0.05). DON-feeding aggravated the porcine acid-base balance in response to a subsequent immunostimulus dependent on its exposure site (pre- or post-hepatic).

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Effect of chronic enteral Fusarium toxin deoxynivalenol (DON) exposure and pre- or post-hepatic E. coli lipopolysaccharide (LPS) infusion on arterial, jugular or portal blood glucose in pigs. Reference value: 70–115 mg/dL in venous blood [26]. Barrows were either fed a DON contaminated ration (4.59 mg/kg feed) or control feed during 29 days. Infusion groups were divided as follows: pre-hepatic LPS infusion (CON_CONjugular-LPSportal, n = 7 and DON_CONjugular-LPSportal, n = 6), post-hepatic LPS infusion (CON_LPSjugular-CONportal, n = 8 and DON_LPSjugular-CONportal, n = 6), and control infusion (CON_CONjugular-CONportal, n = 7 and DON_CONjugular-CONportal, n = 7). Infusion from time 0 until 60 min with 7.5 µg LPS/kg BW in 0.9% saline. Feed was offered during 15 min prior to infusion start. LSMeans. PSEM = 1.75. Significance: Group (G): p = 0.28; Catheter (C): p ≤ 0.001; Time (T): p ≤ 0.001; G × C × T: p ≤ 0.001. Table illustrates differences between DON- and CON-fed control-infused groups at different times. T = trend (p ≤ 0.10).
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toxins-07-04773-f003: Effect of chronic enteral Fusarium toxin deoxynivalenol (DON) exposure and pre- or post-hepatic E. coli lipopolysaccharide (LPS) infusion on arterial, jugular or portal blood glucose in pigs. Reference value: 70–115 mg/dL in venous blood [26]. Barrows were either fed a DON contaminated ration (4.59 mg/kg feed) or control feed during 29 days. Infusion groups were divided as follows: pre-hepatic LPS infusion (CON_CONjugular-LPSportal, n = 7 and DON_CONjugular-LPSportal, n = 6), post-hepatic LPS infusion (CON_LPSjugular-CONportal, n = 8 and DON_LPSjugular-CONportal, n = 6), and control infusion (CON_CONjugular-CONportal, n = 7 and DON_CONjugular-CONportal, n = 7). Infusion from time 0 until 60 min with 7.5 µg LPS/kg BW in 0.9% saline. Feed was offered during 15 min prior to infusion start. LSMeans. PSEM = 1.75. Significance: Group (G): p = 0.28; Catheter (C): p ≤ 0.001; Time (T): p ≤ 0.001; G × C × T: p ≤ 0.001. Table illustrates differences between DON- and CON-fed control-infused groups at different times. T = trend (p ≤ 0.10).

Mentions: A post-prandial increase in glucose until 15–30 min and a subsequent decrease until time 120 min to the base level was observed in all groups (Figure 3), most pronounced at the portal sampling site. The control (CON)-fed animals generally exhibited higher glucose levels at the portal sampling site at 30–45 min (depending on group) than DON-fed animals (Figure 3). The control group (CON_CONjugular-CONportal) maintained elevated post-prandial glucose levels for nearly the entire time course (significantly higher than other groups at 60 min, 90 min and 120 min; p < 0.05 at 120 min).


Metabolic and hematological consequences of dietary deoxynivalenol interacting with systemic Escherichia coli lipopolysaccharide.

Bannert E, Tesch T, Kluess J, Frahm J, Kersten S, Kahlert S, Renner L, Rothkötter HJ, Dänicke S - Toxins (Basel) (2015)

Effect of chronic enteral Fusarium toxin deoxynivalenol (DON) exposure and pre- or post-hepatic E. coli lipopolysaccharide (LPS) infusion on arterial, jugular or portal blood glucose in pigs. Reference value: 70–115 mg/dL in venous blood [26]. Barrows were either fed a DON contaminated ration (4.59 mg/kg feed) or control feed during 29 days. Infusion groups were divided as follows: pre-hepatic LPS infusion (CON_CONjugular-LPSportal, n = 7 and DON_CONjugular-LPSportal, n = 6), post-hepatic LPS infusion (CON_LPSjugular-CONportal, n = 8 and DON_LPSjugular-CONportal, n = 6), and control infusion (CON_CONjugular-CONportal, n = 7 and DON_CONjugular-CONportal, n = 7). Infusion from time 0 until 60 min with 7.5 µg LPS/kg BW in 0.9% saline. Feed was offered during 15 min prior to infusion start. LSMeans. PSEM = 1.75. Significance: Group (G): p = 0.28; Catheter (C): p ≤ 0.001; Time (T): p ≤ 0.001; G × C × T: p ≤ 0.001. Table illustrates differences between DON- and CON-fed control-infused groups at different times. T = trend (p ≤ 0.10).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4663533&req=5

toxins-07-04773-f003: Effect of chronic enteral Fusarium toxin deoxynivalenol (DON) exposure and pre- or post-hepatic E. coli lipopolysaccharide (LPS) infusion on arterial, jugular or portal blood glucose in pigs. Reference value: 70–115 mg/dL in venous blood [26]. Barrows were either fed a DON contaminated ration (4.59 mg/kg feed) or control feed during 29 days. Infusion groups were divided as follows: pre-hepatic LPS infusion (CON_CONjugular-LPSportal, n = 7 and DON_CONjugular-LPSportal, n = 6), post-hepatic LPS infusion (CON_LPSjugular-CONportal, n = 8 and DON_LPSjugular-CONportal, n = 6), and control infusion (CON_CONjugular-CONportal, n = 7 and DON_CONjugular-CONportal, n = 7). Infusion from time 0 until 60 min with 7.5 µg LPS/kg BW in 0.9% saline. Feed was offered during 15 min prior to infusion start. LSMeans. PSEM = 1.75. Significance: Group (G): p = 0.28; Catheter (C): p ≤ 0.001; Time (T): p ≤ 0.001; G × C × T: p ≤ 0.001. Table illustrates differences between DON- and CON-fed control-infused groups at different times. T = trend (p ≤ 0.10).
Mentions: A post-prandial increase in glucose until 15–30 min and a subsequent decrease until time 120 min to the base level was observed in all groups (Figure 3), most pronounced at the portal sampling site. The control (CON)-fed animals generally exhibited higher glucose levels at the portal sampling site at 30–45 min (depending on group) than DON-fed animals (Figure 3). The control group (CON_CONjugular-CONportal) maintained elevated post-prandial glucose levels for nearly the entire time course (significantly higher than other groups at 60 min, 90 min and 120 min; p < 0.05 at 120 min).

Bottom Line: DON-feeding solely decreased portal glucose uptake (p < 0.05).LPS-decreased partial oxygen pressure (pO₂) overall (p < 0.05), but reduced pCO₂ only in arterial blood, and DON had no effect on either.Irrespective of catheter localization, LPS decreased pH and base-excess (p < 0.01), but increased lactate and anion-gap (p < 0.01), indicating an emerging lactic acidosis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, Braunschweig 38116, Germany. Erik.Bannert@fli.bund.de.

ABSTRACT
Previous studies have shown that chronic oral deoxynivalenol (DON) exposure modulated Escherichia coli lipopolysaccharide (LPS)-induced systemic inflammation, whereby the liver was suspected to play an important role. Thus, a total of 41 barrows was fed one of two maize-based diets, either a DON-diet (4.59 mg DON/kg feed, n = 19) or a control diet (CON, n = 22). Pigs were equipped with indwelling catheters for pre- or post-hepatic (portal vs. jugular catheter) infusion of either control (0.9% NaCl) or LPS (7.5 µg/kg BW) for 1h and frequent blood sampling. This design yielded six groups: CON_CONjugular‑CONportal, CON_CONjugular‑LPSportal, CON_LPSjugular‑CONportal, DON_CONjugular‑CONportal, DON_CONjugular‑LPSportal and DON_LPSjugular‑CONportal. Blood samples were analyzed for blood gases, electrolytes, glucose, pH, lactate and red hemogram. The red hemogram and electrolytes were not affected by DON and LPS. DON-feeding solely decreased portal glucose uptake (p < 0.05). LPS-decreased partial oxygen pressure (pO₂) overall (p < 0.05), but reduced pCO₂ only in arterial blood, and DON had no effect on either. Irrespective of catheter localization, LPS decreased pH and base-excess (p < 0.01), but increased lactate and anion-gap (p < 0.01), indicating an emerging lactic acidosis. Lactic acidosis was more pronounced in the group DON_LPSjugular-CONportal than in CON-fed counterparts (p < 0.05). DON-feeding aggravated the porcine acid-base balance in response to a subsequent immunostimulus dependent on its exposure site (pre- or post-hepatic).

Show MeSH
Related in: MedlinePlus