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Studies on the bioavailability of deoxynivalenol (DON) and DON sulfonate (DONS) 1, 2, and 3 in pigs fed with sodium sulfite-treated DON-contaminated maize.

Paulick M, Winkler J, Kersten S, Schatzmayr D, Schwartz-Zimmermann HE, Dänicke S - Toxins (Basel) (2015)

Bottom Line: Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments.The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo.Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. marleen.paulick@fli.bund.de.

ABSTRACT
Deoxynivalenol (DON) exposure of pigs might cause serious problems when critical dietary toxin concentrations are exceeded. As DON contamination of agricultural crops cannot be completely prevented, detoxification measures are needed. Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments. The preserved material had a characteristic DON sulfonates (DONS) pattern. DONS is known to be less toxic than DON but its stability was shown to depend on pH, which gives rise to the question if a back-conversion to DON occurs in vivo. Therefore, the toxicokinetics and bioavailability of DON and DONS were studied in pigs. After the administration of a single oral or intravenous bolus of DON or DONS, serial blood samples were collected and subsequently analyzed. DONS was not detectable after oral administration of DONS mixtures. The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo. Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

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Comparison of plasma concentration-time curves after oral administration of DON containing feed with (SDON) or without (NDON) supplementation of sodium sulfite.
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toxins-07-04622-f003: Comparison of plasma concentration-time curves after oral administration of DON containing feed with (SDON) or without (NDON) supplementation of sodium sulfite.

Mentions: Six pigs fed with an oral single dose of DON contaminated feed (6 mg DON/kg feed) served as the negative DON control group (NDON). A further six pigs were fed with the negative control diet but additionally supplemented with (dry) sodium sulfite immediately before feed consumption (SDON). No signs of toxic effects were observed in either group. In Figure 3, DON plasma concentrations of NDON and SDON (means) were compared. The area under the curve (AUC) of SDON was about 13.9% lower than NDON. The mean half-lives for distribution (t1/2α) for NDON and SDON amounted 2.50 ± 3.27 h and 1.02 ± 0.72 h followed by slower elimination half-lives of 6.05 ± 1.92 h and 5.63 ± 0.94 h, respectively (Table 4). The apparent volume of distribution ranged between 1.13 and 2.66 L/kg for both. The clearance of plasma covered a range from 6.35 to 17.36 mL/kg·min for NDON and 12.62 to 17.53 mL/kg·min for SDON and, therewith, the clearance was nearly two to five times greater than in the DONiv group.


Studies on the bioavailability of deoxynivalenol (DON) and DON sulfonate (DONS) 1, 2, and 3 in pigs fed with sodium sulfite-treated DON-contaminated maize.

Paulick M, Winkler J, Kersten S, Schatzmayr D, Schwartz-Zimmermann HE, Dänicke S - Toxins (Basel) (2015)

Comparison of plasma concentration-time curves after oral administration of DON containing feed with (SDON) or without (NDON) supplementation of sodium sulfite.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663524&req=5

toxins-07-04622-f003: Comparison of plasma concentration-time curves after oral administration of DON containing feed with (SDON) or without (NDON) supplementation of sodium sulfite.
Mentions: Six pigs fed with an oral single dose of DON contaminated feed (6 mg DON/kg feed) served as the negative DON control group (NDON). A further six pigs were fed with the negative control diet but additionally supplemented with (dry) sodium sulfite immediately before feed consumption (SDON). No signs of toxic effects were observed in either group. In Figure 3, DON plasma concentrations of NDON and SDON (means) were compared. The area under the curve (AUC) of SDON was about 13.9% lower than NDON. The mean half-lives for distribution (t1/2α) for NDON and SDON amounted 2.50 ± 3.27 h and 1.02 ± 0.72 h followed by slower elimination half-lives of 6.05 ± 1.92 h and 5.63 ± 0.94 h, respectively (Table 4). The apparent volume of distribution ranged between 1.13 and 2.66 L/kg for both. The clearance of plasma covered a range from 6.35 to 17.36 mL/kg·min for NDON and 12.62 to 17.53 mL/kg·min for SDON and, therewith, the clearance was nearly two to five times greater than in the DONiv group.

Bottom Line: Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments.The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo.Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. marleen.paulick@fli.bund.de.

ABSTRACT
Deoxynivalenol (DON) exposure of pigs might cause serious problems when critical dietary toxin concentrations are exceeded. As DON contamination of agricultural crops cannot be completely prevented, detoxification measures are needed. Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments. The preserved material had a characteristic DON sulfonates (DONS) pattern. DONS is known to be less toxic than DON but its stability was shown to depend on pH, which gives rise to the question if a back-conversion to DON occurs in vivo. Therefore, the toxicokinetics and bioavailability of DON and DONS were studied in pigs. After the administration of a single oral or intravenous bolus of DON or DONS, serial blood samples were collected and subsequently analyzed. DONS was not detectable after oral administration of DONS mixtures. The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo. Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

Show MeSH
Related in: MedlinePlus