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Studies on the bioavailability of deoxynivalenol (DON) and DON sulfonate (DONS) 1, 2, and 3 in pigs fed with sodium sulfite-treated DON-contaminated maize.

Paulick M, Winkler J, Kersten S, Schatzmayr D, Schwartz-Zimmermann HE, Dänicke S - Toxins (Basel) (2015)

Bottom Line: Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments.The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo.Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. marleen.paulick@fli.bund.de.

ABSTRACT
Deoxynivalenol (DON) exposure of pigs might cause serious problems when critical dietary toxin concentrations are exceeded. As DON contamination of agricultural crops cannot be completely prevented, detoxification measures are needed. Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments. The preserved material had a characteristic DON sulfonates (DONS) pattern. DONS is known to be less toxic than DON but its stability was shown to depend on pH, which gives rise to the question if a back-conversion to DON occurs in vivo. Therefore, the toxicokinetics and bioavailability of DON and DONS were studied in pigs. After the administration of a single oral or intravenous bolus of DON or DONS, serial blood samples were collected and subsequently analyzed. DONS was not detectable after oral administration of DONS mixtures. The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo. Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

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Plasma concentration-time curve after intravenous application of 50 µg DON/kg BW to pig IV5.
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toxins-07-04622-f001: Plasma concentration-time curve after intravenous application of 50 µg DON/kg BW to pig IV5.

Mentions: The plasma concentration data from five intravenously dosed pigs were fitted to the bi-exponential regression (Equation (2)) corresponding to a two-compartment model. In Figure 1, an exemplary fitted curve is shown together with the individually-analyzed plasma DON concentrations indicating the typical course after intravenous application. In Table 1 the estimated values, as well as derived toxicokinetic parameters were summarized. The mean half-life (t1/2α) for distribution amounted to 0.09 ± 0.05 h and demonstrated the immediate availability of DON in the systemic cycle. The slower elimination half-life (t1/2β) was, on average, 1.98 ± 0.09 h. The mean apparent volume of distribution (Vd(area)) was 0.61 ± 0.09 L/kg. The plasma clearance averaged 3.61 ± 0.38 mL/kg min.


Studies on the bioavailability of deoxynivalenol (DON) and DON sulfonate (DONS) 1, 2, and 3 in pigs fed with sodium sulfite-treated DON-contaminated maize.

Paulick M, Winkler J, Kersten S, Schatzmayr D, Schwartz-Zimmermann HE, Dänicke S - Toxins (Basel) (2015)

Plasma concentration-time curve after intravenous application of 50 µg DON/kg BW to pig IV5.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663524&req=5

toxins-07-04622-f001: Plasma concentration-time curve after intravenous application of 50 µg DON/kg BW to pig IV5.
Mentions: The plasma concentration data from five intravenously dosed pigs were fitted to the bi-exponential regression (Equation (2)) corresponding to a two-compartment model. In Figure 1, an exemplary fitted curve is shown together with the individually-analyzed plasma DON concentrations indicating the typical course after intravenous application. In Table 1 the estimated values, as well as derived toxicokinetic parameters were summarized. The mean half-life (t1/2α) for distribution amounted to 0.09 ± 0.05 h and demonstrated the immediate availability of DON in the systemic cycle. The slower elimination half-life (t1/2β) was, on average, 1.98 ± 0.09 h. The mean apparent volume of distribution (Vd(area)) was 0.61 ± 0.09 L/kg. The plasma clearance averaged 3.61 ± 0.38 mL/kg min.

Bottom Line: Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments.The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo.Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, 38116 Braunschweig, Germany. marleen.paulick@fli.bund.de.

ABSTRACT
Deoxynivalenol (DON) exposure of pigs might cause serious problems when critical dietary toxin concentrations are exceeded. As DON contamination of agricultural crops cannot be completely prevented, detoxification measures are needed. Wet preservation with sodium sulfite resulted in a significant DON reduction of naturally-contaminated maize in previous experiments. The preserved material had a characteristic DON sulfonates (DONS) pattern. DONS is known to be less toxic than DON but its stability was shown to depend on pH, which gives rise to the question if a back-conversion to DON occurs in vivo. Therefore, the toxicokinetics and bioavailability of DON and DONS were studied in pigs. After the administration of a single oral or intravenous bolus of DON or DONS, serial blood samples were collected and subsequently analyzed. DONS was not detectable after oral administration of DONS mixtures. The results showed further that the bioavailability of DONS as DON in pigs fed maize preserved wet with sodium sulfite was significantly decreased compared to untreated control maize (DON), indicating that DONS obviously did not convert back to DON to a large extent in vivo. Moreover, the fact that DONS was not detectable in systemic blood requires further investigations regarding their ingestive and/or metabolic fate.

Show MeSH
Related in: MedlinePlus