Limits...
The fish diversity in the upper reaches of the Salween River, Nujiang River, revealed by DNA barcoding.

Chen W, Ma X, Shen Y, Mao Y, He S - Sci Rep (2015)

Bottom Line: At the other end of the spectrum, ten species (from three genera) that are characterized by an overlap between their intra- and interspecific genetic distances form a single genetic cluster and share haplotypes.The neighbor-joining phenogram, Barcode Index Numbers (BINs) and Automatic Barcode Gap Discovery (ABGD) identified 43 putative species, while the General Mixed Yule-coalescence (GMYC) identified five more OTUs.Thus, our study established a reliable DNA barcode reference library for the fish in the NR and sheds new light on the local fish diversity.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, China.

ABSTRACT
Nujiang River (NR), an essential component of the biodiversity hotspot of the Mountains of Southwest China, possesses a characteristic fish fauna and contains endemic species. Although previous studies on fish diversity in the NR have primarily consisted of listings of the fish species observed during field collections, in our study, we DNA-barcoded 1139 specimens belonging to 46 morphologically distinct fish species distributed throughout the NR basin by employing multiple analytical approaches. According to our analyses, DNA barcoding is an efficient method for the identification of fish by the presence of barcode gaps. However, three invasive species are characterized by deep conspecific divergences, generating multiple lineages and Operational Taxonomic Units (OTUs), implying the possibility of cryptic species. At the other end of the spectrum, ten species (from three genera) that are characterized by an overlap between their intra- and interspecific genetic distances form a single genetic cluster and share haplotypes. The neighbor-joining phenogram, Barcode Index Numbers (BINs) and Automatic Barcode Gap Discovery (ABGD) identified 43 putative species, while the General Mixed Yule-coalescence (GMYC) identified five more OTUs. Thus, our study established a reliable DNA barcode reference library for the fish in the NR and sheds new light on the local fish diversity.

No MeSH data available.


Related in: MedlinePlus

Haplotype networks of species with low interspecific divergences.(a) Genus Creteuchiloglanis; (b) genus Schistura; and(c) genus Schizothorax. The bold numbers on the internodesindicate mutation steps, and the other numbers are the frequencies of eachhaplotype. A red empty circle indicates missing intermediate steps betweenobserved haplotypes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4663501&req=5

f5: Haplotype networks of species with low interspecific divergences.(a) Genus Creteuchiloglanis; (b) genus Schistura; and(c) genus Schizothorax. The bold numbers on the internodesindicate mutation steps, and the other numbers are the frequencies of eachhaplotype. A red empty circle indicates missing intermediate steps betweenobserved haplotypes.

Mentions: The haplotype networks of the closely related species demonstrated that the sharingof COI haplotypes was common for three genera (Creteuchiloglanis,Schistura, and Schizothorax) (Fig. 5). Themajority of the specimens of the three species in the genus Schizothoraxshared a main haplotype. The genus Creteuchiloglanis generated two groupsseparated by 10 mutation steps; while one group contained specimens of C.macropterus from the tributary, the other included individuals of C.gongshanensis and C. macropterus from the main stem. Additionally,haplotype sharing was popular among C. gongshanensis and C.macropterus in the main stem. Five closely related species inSchistura formed two groups as well; one group only containedSchistura longa, and the other group consisted of five species(Schistura longa, S. poculi, S. prolixifasciata, S.sp., and S. vinciguerrae).


The fish diversity in the upper reaches of the Salween River, Nujiang River, revealed by DNA barcoding.

Chen W, Ma X, Shen Y, Mao Y, He S - Sci Rep (2015)

Haplotype networks of species with low interspecific divergences.(a) Genus Creteuchiloglanis; (b) genus Schistura; and(c) genus Schizothorax. The bold numbers on the internodesindicate mutation steps, and the other numbers are the frequencies of eachhaplotype. A red empty circle indicates missing intermediate steps betweenobserved haplotypes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663501&req=5

f5: Haplotype networks of species with low interspecific divergences.(a) Genus Creteuchiloglanis; (b) genus Schistura; and(c) genus Schizothorax. The bold numbers on the internodesindicate mutation steps, and the other numbers are the frequencies of eachhaplotype. A red empty circle indicates missing intermediate steps betweenobserved haplotypes.
Mentions: The haplotype networks of the closely related species demonstrated that the sharingof COI haplotypes was common for three genera (Creteuchiloglanis,Schistura, and Schizothorax) (Fig. 5). Themajority of the specimens of the three species in the genus Schizothoraxshared a main haplotype. The genus Creteuchiloglanis generated two groupsseparated by 10 mutation steps; while one group contained specimens of C.macropterus from the tributary, the other included individuals of C.gongshanensis and C. macropterus from the main stem. Additionally,haplotype sharing was popular among C. gongshanensis and C.macropterus in the main stem. Five closely related species inSchistura formed two groups as well; one group only containedSchistura longa, and the other group consisted of five species(Schistura longa, S. poculi, S. prolixifasciata, S.sp., and S. vinciguerrae).

Bottom Line: At the other end of the spectrum, ten species (from three genera) that are characterized by an overlap between their intra- and interspecific genetic distances form a single genetic cluster and share haplotypes.The neighbor-joining phenogram, Barcode Index Numbers (BINs) and Automatic Barcode Gap Discovery (ABGD) identified 43 putative species, while the General Mixed Yule-coalescence (GMYC) identified five more OTUs.Thus, our study established a reliable DNA barcode reference library for the fish in the NR and sheds new light on the local fish diversity.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, China.

ABSTRACT
Nujiang River (NR), an essential component of the biodiversity hotspot of the Mountains of Southwest China, possesses a characteristic fish fauna and contains endemic species. Although previous studies on fish diversity in the NR have primarily consisted of listings of the fish species observed during field collections, in our study, we DNA-barcoded 1139 specimens belonging to 46 morphologically distinct fish species distributed throughout the NR basin by employing multiple analytical approaches. According to our analyses, DNA barcoding is an efficient method for the identification of fish by the presence of barcode gaps. However, three invasive species are characterized by deep conspecific divergences, generating multiple lineages and Operational Taxonomic Units (OTUs), implying the possibility of cryptic species. At the other end of the spectrum, ten species (from three genera) that are characterized by an overlap between their intra- and interspecific genetic distances form a single genetic cluster and share haplotypes. The neighbor-joining phenogram, Barcode Index Numbers (BINs) and Automatic Barcode Gap Discovery (ABGD) identified 43 putative species, while the General Mixed Yule-coalescence (GMYC) identified five more OTUs. Thus, our study established a reliable DNA barcode reference library for the fish in the NR and sheds new light on the local fish diversity.

No MeSH data available.


Related in: MedlinePlus