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Zeolite Nanoparticles for Selective Sorption of Plasma Proteins.

Rahimi M, Ng EP, Bakhtiari K, Vinciguerra M, Ali Ahmad H, Awala H, Mintova S, Daghighi M, Bakhshandeh Rostami F, de Vries M, Motazacker MM, Peppelenbosch MP, Mahmoudi M, Rezaee F - Sci Rep (2015)

Bottom Line: While the zeolite nanoparticles exposed to low plasma concentration (10%) exhibited a high selective adsorption for immunoglobulin gamma (i.e. IGHG1, IGHG2 and IGHG4) proteins.The zeolite nanoparticles can potentially be used for selectively capture of APOC-III in order to reduce the activation of lipoprotein lipase inhibition during hypertriglyceridemia treatment.The zeolite nanoparticles can be adapted to hemophilic patients (hemophilia A (F-VIII deficient) and hemophilia B (F-IX deficient)) with a risk of bleeding, and thus might be potentially used in combination with the existing therapy.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.

ABSTRACT
The affinity of zeolite nanoparticles (diameter of 8-12 nm) possessing high surface area and high pore volume towards human plasma proteins has been investigated. The protein composition (corona) of zeolite nanoparticles has been shown to be more dependent on the plasma protein concentrations and the type of zeolites than zeolite nanoparticles concentration. The number of proteins present in the corona of zeolite nanoparticles at 100% plasma (in vivo state) is less than with 10% plasma exposure. This could be due to a competition between the proteins to occupy the corona of the zeolite nanoparticles. Moreover, a high selective adsorption for apolipoprotein C-III (APOC-III) and fibrinogen on the zeolite nanoparticles at high plasma concentration (100%) was observed. While the zeolite nanoparticles exposed to low plasma concentration (10%) exhibited a high selective adsorption for immunoglobulin gamma (i.e. IGHG1, IGHG2 and IGHG4) proteins. The zeolite nanoparticles can potentially be used for selectively capture of APOC-III in order to reduce the activation of lipoprotein lipase inhibition during hypertriglyceridemia treatment. The zeolite nanoparticles can be adapted to hemophilic patients (hemophilia A (F-VIII deficient) and hemophilia B (F-IX deficient)) with a risk of bleeding, and thus might be potentially used in combination with the existing therapy.

No MeSH data available.


Related in: MedlinePlus

nLC-MS/MS analysis of corona-associated proteins on FAU-zeolite nanoparticles.Apolipoprotein C-III (APOC-III), fibrinogen alpha chain (FIBA), fibrinogen beta chain (FIBB), fibrinogen gamma chain (FIBG), albumin (ALBU), IGHG1, IGHG2 and IGHG4.
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f4: nLC-MS/MS analysis of corona-associated proteins on FAU-zeolite nanoparticles.Apolipoprotein C-III (APOC-III), fibrinogen alpha chain (FIBA), fibrinogen beta chain (FIBB), fibrinogen gamma chain (FIBG), albumin (ALBU), IGHG1, IGHG2 and IGHG4.

Mentions: The affinity of the EMT- and FAU- zeolite nanoparticles towards human plasma proteins was investigated by incubating zeolite nanoparticles with plasma proteins using one of the following strategies: (1) the concentration of nanoparticles was kept constant but the plasma concentration was varied, and (2) the plasma concentration was kept constant but the concentration of zeolite nanoparticles was varied. The identification of proteins present on the surface of EMT- and FAU-zeolite nanoparticles (SI: Tables S1 and S2) was carried out using nLC-MS/MS (LTQ-ORBITRAP-XL; tandem mass spectrometry) combined with PEAKS DB (software) analysis. The nLC-MS/MS results show that with increasing the plasma concentrations from 10% to 100%, the APOC-III and the three chains of fibrinogen (FIBA, FIBB and FIBG) are bound on the surface of the zeolite nanoparticles. The amount of adsorption was less dependent on the concentration of zeolite nanoparticles than plasma concentrations used (Figures 3 and 4; and SI: Tables S1 and S2). After exposure of zeolite nanoparticles to very low plasma concentrations (10%), the zeolites exhibit a high affinity for immunoglobulin gamma (i.e. IGHG1, IGHG2 and IGHG4) as compared to the high plasma concentrations (Figures 3 and 4). Notably, upon using EMT-zeolite nanoparticles with 100% plasma, the APOC-III showed an adsorption of 30%. Furthermore, all three chains of fibrinogen (FIBA, FIBB, and FIBG) demonstrated a high affinity toward EMT-zeolite nanoparticles even at low concentrations (4–12%) incubated with 10% plasma. As a representative example, the spectra of Fibrinogen α-chain and APOC-III after injection of the extracted peptides from EMT and FAU zeolite nanoparticles are depicted in Figure 5 (a,b).


Zeolite Nanoparticles for Selective Sorption of Plasma Proteins.

Rahimi M, Ng EP, Bakhtiari K, Vinciguerra M, Ali Ahmad H, Awala H, Mintova S, Daghighi M, Bakhshandeh Rostami F, de Vries M, Motazacker MM, Peppelenbosch MP, Mahmoudi M, Rezaee F - Sci Rep (2015)

nLC-MS/MS analysis of corona-associated proteins on FAU-zeolite nanoparticles.Apolipoprotein C-III (APOC-III), fibrinogen alpha chain (FIBA), fibrinogen beta chain (FIBB), fibrinogen gamma chain (FIBG), albumin (ALBU), IGHG1, IGHG2 and IGHG4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663482&req=5

f4: nLC-MS/MS analysis of corona-associated proteins on FAU-zeolite nanoparticles.Apolipoprotein C-III (APOC-III), fibrinogen alpha chain (FIBA), fibrinogen beta chain (FIBB), fibrinogen gamma chain (FIBG), albumin (ALBU), IGHG1, IGHG2 and IGHG4.
Mentions: The affinity of the EMT- and FAU- zeolite nanoparticles towards human plasma proteins was investigated by incubating zeolite nanoparticles with plasma proteins using one of the following strategies: (1) the concentration of nanoparticles was kept constant but the plasma concentration was varied, and (2) the plasma concentration was kept constant but the concentration of zeolite nanoparticles was varied. The identification of proteins present on the surface of EMT- and FAU-zeolite nanoparticles (SI: Tables S1 and S2) was carried out using nLC-MS/MS (LTQ-ORBITRAP-XL; tandem mass spectrometry) combined with PEAKS DB (software) analysis. The nLC-MS/MS results show that with increasing the plasma concentrations from 10% to 100%, the APOC-III and the three chains of fibrinogen (FIBA, FIBB and FIBG) are bound on the surface of the zeolite nanoparticles. The amount of adsorption was less dependent on the concentration of zeolite nanoparticles than plasma concentrations used (Figures 3 and 4; and SI: Tables S1 and S2). After exposure of zeolite nanoparticles to very low plasma concentrations (10%), the zeolites exhibit a high affinity for immunoglobulin gamma (i.e. IGHG1, IGHG2 and IGHG4) as compared to the high plasma concentrations (Figures 3 and 4). Notably, upon using EMT-zeolite nanoparticles with 100% plasma, the APOC-III showed an adsorption of 30%. Furthermore, all three chains of fibrinogen (FIBA, FIBB, and FIBG) demonstrated a high affinity toward EMT-zeolite nanoparticles even at low concentrations (4–12%) incubated with 10% plasma. As a representative example, the spectra of Fibrinogen α-chain and APOC-III after injection of the extracted peptides from EMT and FAU zeolite nanoparticles are depicted in Figure 5 (a,b).

Bottom Line: While the zeolite nanoparticles exposed to low plasma concentration (10%) exhibited a high selective adsorption for immunoglobulin gamma (i.e. IGHG1, IGHG2 and IGHG4) proteins.The zeolite nanoparticles can potentially be used for selectively capture of APOC-III in order to reduce the activation of lipoprotein lipase inhibition during hypertriglyceridemia treatment.The zeolite nanoparticles can be adapted to hemophilic patients (hemophilia A (F-VIII deficient) and hemophilia B (F-IX deficient)) with a risk of bleeding, and thus might be potentially used in combination with the existing therapy.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.

ABSTRACT
The affinity of zeolite nanoparticles (diameter of 8-12 nm) possessing high surface area and high pore volume towards human plasma proteins has been investigated. The protein composition (corona) of zeolite nanoparticles has been shown to be more dependent on the plasma protein concentrations and the type of zeolites than zeolite nanoparticles concentration. The number of proteins present in the corona of zeolite nanoparticles at 100% plasma (in vivo state) is less than with 10% plasma exposure. This could be due to a competition between the proteins to occupy the corona of the zeolite nanoparticles. Moreover, a high selective adsorption for apolipoprotein C-III (APOC-III) and fibrinogen on the zeolite nanoparticles at high plasma concentration (100%) was observed. While the zeolite nanoparticles exposed to low plasma concentration (10%) exhibited a high selective adsorption for immunoglobulin gamma (i.e. IGHG1, IGHG2 and IGHG4) proteins. The zeolite nanoparticles can potentially be used for selectively capture of APOC-III in order to reduce the activation of lipoprotein lipase inhibition during hypertriglyceridemia treatment. The zeolite nanoparticles can be adapted to hemophilic patients (hemophilia A (F-VIII deficient) and hemophilia B (F-IX deficient)) with a risk of bleeding, and thus might be potentially used in combination with the existing therapy.

No MeSH data available.


Related in: MedlinePlus