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Effects of a discoloration-resistant calcium aluminosilicate cement on the viability and proliferation of undifferentiated human dental pulp stem cells.

Niu LN, Watson D, Thames K, Primus CM, Bergeron BE, Jiao K, Bortoluzzi EA, Cutler CW, Chen JH, Pashley DH, Tay FR - Sci Rep (2015)

Bottom Line: Discoloration-resistant calcium aluminosilicate cement has been formulated to overcome the timely problem of tooth discoloration reported in the clinical application of bismuth oxide-containing hydraulic cements.The present study examined the effects of this experimental cement (Quick-Set2) on the viability and proliferation of human dental pulp stem cells (hDPSCs) by comparing the cellular responses with commercially available calcium silicate cement (white mineral trioxide aggregate; WMTA) after different aging periods.Based on these results, it is envisaged that any potential beneficial effect of the discoloration-resistant calcium aluminosilicate cement on osteogenesis by differentiated hDPSCs is more likely to be revealed after outward diffusion and removal of its cytotoxic components.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Military Stomatology, School of Stomatology, Department of Prosthodontics, Fourth Military Medical University, Xi'an, Shaanxi, China.

ABSTRACT
Discoloration-resistant calcium aluminosilicate cement has been formulated to overcome the timely problem of tooth discoloration reported in the clinical application of bismuth oxide-containing hydraulic cements. The present study examined the effects of this experimental cement (Quick-Set2) on the viability and proliferation of human dental pulp stem cells (hDPSCs) by comparing the cellular responses with commercially available calcium silicate cement (white mineral trioxide aggregate; WMTA) after different aging periods. Cell viability and proliferation were examined using assays that examined plasma membrane integrity, leakage of cytosolic enzyme, caspase-3 activity for early apoptosis, oxidative stress, mitochondrial metabolic activity and intracellular DNA content. Results of the six assays indicated that both Quick-Set2 and WMTA were initially cytotoxic to hDPSCs after setting for 24 h, with Quick-Set2 being comparatively less cytotoxic than WMTA at this stage. After two aging cycles, the cytotoxicity profiles of the two hydraulic cements were not significantly different and were much less cytotoxic than the positive control (zinc oxide-eugenol cement). Based on these results, it is envisaged that any potential beneficial effect of the discoloration-resistant calcium aluminosilicate cement on osteogenesis by differentiated hDPSCs is more likely to be revealed after outward diffusion and removal of its cytotoxic components.

No MeSH data available.


Related in: MedlinePlus

Resistance to discoloration.Color changes in white ProRoot MTA (WMTA) and Quick-Set2 after the set materials were incubated in deionized water (DI), 2% chlorhexidine (CHX), 8.25% sodium hypochlorite (NaOCl) and 10% formaldehyde (FADH) for 7 days.
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f1: Resistance to discoloration.Color changes in white ProRoot MTA (WMTA) and Quick-Set2 after the set materials were incubated in deionized water (DI), 2% chlorhexidine (CHX), 8.25% sodium hypochlorite (NaOCl) and 10% formaldehyde (FADH) for 7 days.

Mentions: Color changes after the set hydraulic cements were incubated in different solutions (Fig. 1) is an indirect evidence for the possibility of tooth discoloration caused by the material. The color of white ProRoot MTA (WMTA) remained stable after immersion in deionized water. Although no spectrophotometric methods were employed, color changes were intense enough to be visibly discerned after WMTA were incubated in the other solutions; specimens turned gray after immersion in 2% chlorhexidine, dark brown after immersion in 8.25% sodium hypochlorite and black after immersion in 10% formaldehyde. These color changes are similar to what had been reported in the literature for calcium silicate cements that utilized bismuth oxide as radiopacifier252629. Sodium hypochlorite and chlorhexidine are commonly used irrigants in root canal treatment. Although there is no indication for formaldehyde in clinical practice, WMTA root end fillings performed in animal studies turned black after fixation with formaldehyde during preparation of histological sections (Primus, unpublished results). In comparison, Quick-Set2 was resistant to discoloration after the specimens were incubated in the different solutions. The results suggest that tantalum oxide may be a better alternative than bismuth oxide as a color-stable radiopacifier for hydraulic cements that are designed for clinical procedures involving the coronal aspect of a tooth.


Effects of a discoloration-resistant calcium aluminosilicate cement on the viability and proliferation of undifferentiated human dental pulp stem cells.

Niu LN, Watson D, Thames K, Primus CM, Bergeron BE, Jiao K, Bortoluzzi EA, Cutler CW, Chen JH, Pashley DH, Tay FR - Sci Rep (2015)

Resistance to discoloration.Color changes in white ProRoot MTA (WMTA) and Quick-Set2 after the set materials were incubated in deionized water (DI), 2% chlorhexidine (CHX), 8.25% sodium hypochlorite (NaOCl) and 10% formaldehyde (FADH) for 7 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663481&req=5

f1: Resistance to discoloration.Color changes in white ProRoot MTA (WMTA) and Quick-Set2 after the set materials were incubated in deionized water (DI), 2% chlorhexidine (CHX), 8.25% sodium hypochlorite (NaOCl) and 10% formaldehyde (FADH) for 7 days.
Mentions: Color changes after the set hydraulic cements were incubated in different solutions (Fig. 1) is an indirect evidence for the possibility of tooth discoloration caused by the material. The color of white ProRoot MTA (WMTA) remained stable after immersion in deionized water. Although no spectrophotometric methods were employed, color changes were intense enough to be visibly discerned after WMTA were incubated in the other solutions; specimens turned gray after immersion in 2% chlorhexidine, dark brown after immersion in 8.25% sodium hypochlorite and black after immersion in 10% formaldehyde. These color changes are similar to what had been reported in the literature for calcium silicate cements that utilized bismuth oxide as radiopacifier252629. Sodium hypochlorite and chlorhexidine are commonly used irrigants in root canal treatment. Although there is no indication for formaldehyde in clinical practice, WMTA root end fillings performed in animal studies turned black after fixation with formaldehyde during preparation of histological sections (Primus, unpublished results). In comparison, Quick-Set2 was resistant to discoloration after the specimens were incubated in the different solutions. The results suggest that tantalum oxide may be a better alternative than bismuth oxide as a color-stable radiopacifier for hydraulic cements that are designed for clinical procedures involving the coronal aspect of a tooth.

Bottom Line: Discoloration-resistant calcium aluminosilicate cement has been formulated to overcome the timely problem of tooth discoloration reported in the clinical application of bismuth oxide-containing hydraulic cements.The present study examined the effects of this experimental cement (Quick-Set2) on the viability and proliferation of human dental pulp stem cells (hDPSCs) by comparing the cellular responses with commercially available calcium silicate cement (white mineral trioxide aggregate; WMTA) after different aging periods.Based on these results, it is envisaged that any potential beneficial effect of the discoloration-resistant calcium aluminosilicate cement on osteogenesis by differentiated hDPSCs is more likely to be revealed after outward diffusion and removal of its cytotoxic components.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Military Stomatology, School of Stomatology, Department of Prosthodontics, Fourth Military Medical University, Xi'an, Shaanxi, China.

ABSTRACT
Discoloration-resistant calcium aluminosilicate cement has been formulated to overcome the timely problem of tooth discoloration reported in the clinical application of bismuth oxide-containing hydraulic cements. The present study examined the effects of this experimental cement (Quick-Set2) on the viability and proliferation of human dental pulp stem cells (hDPSCs) by comparing the cellular responses with commercially available calcium silicate cement (white mineral trioxide aggregate; WMTA) after different aging periods. Cell viability and proliferation were examined using assays that examined plasma membrane integrity, leakage of cytosolic enzyme, caspase-3 activity for early apoptosis, oxidative stress, mitochondrial metabolic activity and intracellular DNA content. Results of the six assays indicated that both Quick-Set2 and WMTA were initially cytotoxic to hDPSCs after setting for 24 h, with Quick-Set2 being comparatively less cytotoxic than WMTA at this stage. After two aging cycles, the cytotoxicity profiles of the two hydraulic cements were not significantly different and were much less cytotoxic than the positive control (zinc oxide-eugenol cement). Based on these results, it is envisaged that any potential beneficial effect of the discoloration-resistant calcium aluminosilicate cement on osteogenesis by differentiated hDPSCs is more likely to be revealed after outward diffusion and removal of its cytotoxic components.

No MeSH data available.


Related in: MedlinePlus