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Antinociceptive and Anti-Inflammatory Activities of Teucrium persicum Boiss. Extract in Mice.

Miri A, Sharifi-Rad J, Tabrizian K, Nasiri AA - Scientifica (Cairo) (2015)

Bottom Line: The TPEs did not have any significant anti-inflammatory effects in cotton pellet-induced granuloma formation in mice.Conclusions.These results suggest that the aqueous extract from T. persicum Boiss. produced antinociceptive effects.

View Article: PubMed Central - PubMed

Affiliation: Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran ; Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.

ABSTRACT
Background. Therapeutic properties of Teucrium species as antioxidant, antibacterial, analgesic, anticancer, diuretic, and tonic compounds have been proved earlier. Materials and Methods. In this study, the antinociceptive and anti-inflammatory effects of the aqueous extract of Teucrium persicum on chronic pain, sciatic nerve ligation as a model of neuropathic pain, and inflammatory models were investigated by formalin, hot-plate, and cotton pellet-induced granuloma models in mice, respectively. T. persicum aqueous extracts (100, 200, and 400 mg/kg) were orally gavaged for one week. On 8th day, the time spent and the number of lickings were recorded in formalin test. Morphine and Diclofenac were used intraperitoneally as positive controls. In sciatic nerve ligated animals, as a model of neuropathic pain, doses (100, 200, and 400 mg/kg) of T. persicum extract (TPE) were orally gavaged for 14 consecutive days. The analgesic effect of this extract was examined 14 days after sciatic nerve ligation using the hot-plate test. Controls received saline and Imipramine (40 mg/kg, i.p.) was used a positive control for neuropathic pain model. Results. In the formalin test, a week oral gavage of all TPE doses (100, 200, and 400 mg/kg) caused a significant decrease on the licking response compared to the control negative animals. In the hot-plate test, doses of 200 and 400 mg/kg showed significant analgesic effects in sciatic nerve ligated animals. Oral gavaged of TPE revealed significant analgesic effect on chronic pain in both formalin test and sciatic nerve ligated animals. The TPEs did not have any significant anti-inflammatory effects in cotton pellet-induced granuloma formation in mice. Conclusions. These results suggest that the aqueous extract from T. persicum Boiss. produced antinociceptive effects. Its exact mechanism of action still remains indistinct.

No MeSH data available.


Related in: MedlinePlus

Effects of TPE, Morphine (9 mg/kg), and Diclofenac (10 mg/kg) in the chronic phase of the formalin test in mice by assessing the number of lickings (a) and the licking time (b). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 significantly different from the control animals. Each value represents the mean ± SEM (n = 8).
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fig2: Effects of TPE, Morphine (9 mg/kg), and Diclofenac (10 mg/kg) in the chronic phase of the formalin test in mice by assessing the number of lickings (a) and the licking time (b). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 significantly different from the control animals. Each value represents the mean ± SEM (n = 8).

Mentions: As shown in Figure 1, the evaluation of the licking response in the acute phase of the formalin test showed that gavage consecutively of TPE (100, 200, and 400 mg/kg) for 7 days caused a significant decrease on the licking response in comparison with the control groups. There was no significant difference between tested group and control except Morphine (∗P < 0.05). However in chronic phase, Morphine (∗P < 0.05) and Diclofenac (∗∗P < 0.01) exerted significant decrease on the licking response compared to control animals. Also both LT and LN showed a statistically significant difference in the groups receiving the plant extract (100, 200, and 400 mg/kg) compared with control group for LT and LN (∗∗P < 0.01 for 100 mg/kg for LT and LN; ∗∗P < 0.01 and ∗∗∗P < 0.001 for 200 and 400 mg/kg for LT and LN, resp.) (Figure 2).


Antinociceptive and Anti-Inflammatory Activities of Teucrium persicum Boiss. Extract in Mice.

Miri A, Sharifi-Rad J, Tabrizian K, Nasiri AA - Scientifica (Cairo) (2015)

Effects of TPE, Morphine (9 mg/kg), and Diclofenac (10 mg/kg) in the chronic phase of the formalin test in mice by assessing the number of lickings (a) and the licking time (b). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 significantly different from the control animals. Each value represents the mean ± SEM (n = 8).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663370&req=5

fig2: Effects of TPE, Morphine (9 mg/kg), and Diclofenac (10 mg/kg) in the chronic phase of the formalin test in mice by assessing the number of lickings (a) and the licking time (b). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 significantly different from the control animals. Each value represents the mean ± SEM (n = 8).
Mentions: As shown in Figure 1, the evaluation of the licking response in the acute phase of the formalin test showed that gavage consecutively of TPE (100, 200, and 400 mg/kg) for 7 days caused a significant decrease on the licking response in comparison with the control groups. There was no significant difference between tested group and control except Morphine (∗P < 0.05). However in chronic phase, Morphine (∗P < 0.05) and Diclofenac (∗∗P < 0.01) exerted significant decrease on the licking response compared to control animals. Also both LT and LN showed a statistically significant difference in the groups receiving the plant extract (100, 200, and 400 mg/kg) compared with control group for LT and LN (∗∗P < 0.01 for 100 mg/kg for LT and LN; ∗∗P < 0.01 and ∗∗∗P < 0.001 for 200 and 400 mg/kg for LT and LN, resp.) (Figure 2).

Bottom Line: The TPEs did not have any significant anti-inflammatory effects in cotton pellet-induced granuloma formation in mice.Conclusions.These results suggest that the aqueous extract from T. persicum Boiss. produced antinociceptive effects.

View Article: PubMed Central - PubMed

Affiliation: Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran ; Department of Pharmacognosy, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.

ABSTRACT
Background. Therapeutic properties of Teucrium species as antioxidant, antibacterial, analgesic, anticancer, diuretic, and tonic compounds have been proved earlier. Materials and Methods. In this study, the antinociceptive and anti-inflammatory effects of the aqueous extract of Teucrium persicum on chronic pain, sciatic nerve ligation as a model of neuropathic pain, and inflammatory models were investigated by formalin, hot-plate, and cotton pellet-induced granuloma models in mice, respectively. T. persicum aqueous extracts (100, 200, and 400 mg/kg) were orally gavaged for one week. On 8th day, the time spent and the number of lickings were recorded in formalin test. Morphine and Diclofenac were used intraperitoneally as positive controls. In sciatic nerve ligated animals, as a model of neuropathic pain, doses (100, 200, and 400 mg/kg) of T. persicum extract (TPE) were orally gavaged for 14 consecutive days. The analgesic effect of this extract was examined 14 days after sciatic nerve ligation using the hot-plate test. Controls received saline and Imipramine (40 mg/kg, i.p.) was used a positive control for neuropathic pain model. Results. In the formalin test, a week oral gavage of all TPE doses (100, 200, and 400 mg/kg) caused a significant decrease on the licking response compared to the control negative animals. In the hot-plate test, doses of 200 and 400 mg/kg showed significant analgesic effects in sciatic nerve ligated animals. Oral gavaged of TPE revealed significant analgesic effect on chronic pain in both formalin test and sciatic nerve ligated animals. The TPEs did not have any significant anti-inflammatory effects in cotton pellet-induced granuloma formation in mice. Conclusions. These results suggest that the aqueous extract from T. persicum Boiss. produced antinociceptive effects. Its exact mechanism of action still remains indistinct.

No MeSH data available.


Related in: MedlinePlus