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Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats.

Zhao X, Qiu X, Zhang Y, Zhang S, Gu X, Guo H - Stem Cells Int (2015)

Bottom Line: Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs.In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer.Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China ; Institute of Urology, Nanjing University, Nanjing 210093, China.

ABSTRACT
It has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance the therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D) aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer. Furthermore, AdMSCs in 3D aggregates secreted more proangiogenic factors than those cultured in 2D monolayer. 2D AdMSCs or 3D AdMSCs aggregates were injected into renal cortex immediately after induction of renal IR injury. In vivo data revealed that 3D aggregates enhanced the effects of AdMSCs in recovering function and structure after renal IR injury. Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer. Our results demonstrated that 3D AdMSCs aggregated produced by microwell enhanced the retention and therapeutic effects of AdMSCs for renal IR injury.

No MeSH data available.


Related in: MedlinePlus

Retention of AdMSCs in kidney. (a) Representative images of EdU-labeled AdMSCs (indicated by white arrow) identified in kidney of rats underwent injection of 2D AdMSCs or 3D aggregates. (b) Results of EdU positive AdMSCs quantification in each high power field (HPF). Data were normalized to IR + AdMSCs group. ∗P < 0.05 compared to IR + AdMSCs group.
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fig6: Retention of AdMSCs in kidney. (a) Representative images of EdU-labeled AdMSCs (indicated by white arrow) identified in kidney of rats underwent injection of 2D AdMSCs or 3D aggregates. (b) Results of EdU positive AdMSCs quantification in each high power field (HPF). Data were normalized to IR + AdMSCs group. ∗P < 0.05 compared to IR + AdMSCs group.

Mentions: The number of EdU positive AdMSCs in the renal section was counted to determine cell engraftment after injection. As shown in Figure 6, the number of EdU positive cells in the renal section from 3D aggregates group was significantly greater compared with that in 2D monolayer, suggesting that the form of 3D aggregates exhibit greater survival when implanted in vivo following renal IR injury.


Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats.

Zhao X, Qiu X, Zhang Y, Zhang S, Gu X, Guo H - Stem Cells Int (2015)

Retention of AdMSCs in kidney. (a) Representative images of EdU-labeled AdMSCs (indicated by white arrow) identified in kidney of rats underwent injection of 2D AdMSCs or 3D aggregates. (b) Results of EdU positive AdMSCs quantification in each high power field (HPF). Data were normalized to IR + AdMSCs group. ∗P < 0.05 compared to IR + AdMSCs group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663369&req=5

fig6: Retention of AdMSCs in kidney. (a) Representative images of EdU-labeled AdMSCs (indicated by white arrow) identified in kidney of rats underwent injection of 2D AdMSCs or 3D aggregates. (b) Results of EdU positive AdMSCs quantification in each high power field (HPF). Data were normalized to IR + AdMSCs group. ∗P < 0.05 compared to IR + AdMSCs group.
Mentions: The number of EdU positive AdMSCs in the renal section was counted to determine cell engraftment after injection. As shown in Figure 6, the number of EdU positive cells in the renal section from 3D aggregates group was significantly greater compared with that in 2D monolayer, suggesting that the form of 3D aggregates exhibit greater survival when implanted in vivo following renal IR injury.

Bottom Line: Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs.In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer.Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China ; Institute of Urology, Nanjing University, Nanjing 210093, China.

ABSTRACT
It has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance the therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D) aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer. Furthermore, AdMSCs in 3D aggregates secreted more proangiogenic factors than those cultured in 2D monolayer. 2D AdMSCs or 3D AdMSCs aggregates were injected into renal cortex immediately after induction of renal IR injury. In vivo data revealed that 3D aggregates enhanced the effects of AdMSCs in recovering function and structure after renal IR injury. Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer. Our results demonstrated that 3D AdMSCs aggregated produced by microwell enhanced the retention and therapeutic effects of AdMSCs for renal IR injury.

No MeSH data available.


Related in: MedlinePlus