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Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats.

Zhao X, Qiu X, Zhang Y, Zhang S, Gu X, Guo H - Stem Cells Int (2015)

Bottom Line: Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs.In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer.Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China ; Institute of Urology, Nanjing University, Nanjing 210093, China.

ABSTRACT
It has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance the therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D) aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer. Furthermore, AdMSCs in 3D aggregates secreted more proangiogenic factors than those cultured in 2D monolayer. 2D AdMSCs or 3D AdMSCs aggregates were injected into renal cortex immediately after induction of renal IR injury. In vivo data revealed that 3D aggregates enhanced the effects of AdMSCs in recovering function and structure after renal IR injury. Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer. Our results demonstrated that 3D AdMSCs aggregated produced by microwell enhanced the retention and therapeutic effects of AdMSCs for renal IR injury.

No MeSH data available.


Related in: MedlinePlus

2D AdMSCs or 3D aggregate reduces tubular apoptosis after IR. (a) Representative images of TUNEL staining of kidney sections in each experimental group. White arrow indicated apoptotic cells in tubules. (b) Results of the number of apoptotic cells in kidney sections in each group. ∗P < 0.05.
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fig5: 2D AdMSCs or 3D aggregate reduces tubular apoptosis after IR. (a) Representative images of TUNEL staining of kidney sections in each experimental group. White arrow indicated apoptotic cells in tubules. (b) Results of the number of apoptotic cells in kidney sections in each group. ∗P < 0.05.

Mentions: To investigate IR associated apoptotic cells, we measured TUNEL positive cells in kidney tissues. As shown in Figure 5, 24 hours after IR, no apoptotic cells were observed in the kidney from sham group. The number of apoptotic cells increased significantly in IR group compared with sham group. In contrast, tissues from AdMSCs and aggregates groups contain a significantly smaller number of TUNEL positive apoptotic cells. Furthermore, the number of apoptotic cells in 3D aggregates group reduced significantly in 3D aggregates group compared with that in 2D AdMSCs.


Three-Dimensional Aggregates Enhance the Therapeutic Effects of Adipose Mesenchymal Stem Cells for Ischemia-Reperfusion Induced Kidney Injury in Rats.

Zhao X, Qiu X, Zhang Y, Zhang S, Gu X, Guo H - Stem Cells Int (2015)

2D AdMSCs or 3D aggregate reduces tubular apoptosis after IR. (a) Representative images of TUNEL staining of kidney sections in each experimental group. White arrow indicated apoptotic cells in tubules. (b) Results of the number of apoptotic cells in kidney sections in each group. ∗P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4663369&req=5

fig5: 2D AdMSCs or 3D aggregate reduces tubular apoptosis after IR. (a) Representative images of TUNEL staining of kidney sections in each experimental group. White arrow indicated apoptotic cells in tubules. (b) Results of the number of apoptotic cells in kidney sections in each group. ∗P < 0.05.
Mentions: To investigate IR associated apoptotic cells, we measured TUNEL positive cells in kidney tissues. As shown in Figure 5, 24 hours after IR, no apoptotic cells were observed in the kidney from sham group. The number of apoptotic cells increased significantly in IR group compared with sham group. In contrast, tissues from AdMSCs and aggregates groups contain a significantly smaller number of TUNEL positive apoptotic cells. Furthermore, the number of apoptotic cells in 3D aggregates group reduced significantly in 3D aggregates group compared with that in 2D AdMSCs.

Bottom Line: Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs.In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer.Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China ; Institute of Urology, Nanjing University, Nanjing 210093, China.

ABSTRACT
It has been shown that administration of adipose derived mesenchymal stem cells (AdMSCs) enhanced structural and functional recovery of renal ischemia-reperfusion (IR) injury. Low engraftment of stem cells, however, limits the therapeutic effects of AdMSCs. The present study was designed to enhance the therapeutic effects of AdMSCs by delivering AdMSCs in a three-dimensional (3D) aggregates form. Microwell was used to produce 3D AdMSCs aggregates. In vitro data indicated that AdMSCs in 3D aggregates were less susceptible to oxidative and hypoxia stress induced by 200 μM peroxide and hypoxia/reoxygenation, respectively, compared with those cultured in two-dimensional (2D) monolayer. Furthermore, AdMSCs in 3D aggregates secreted more proangiogenic factors than those cultured in 2D monolayer. 2D AdMSCs or 3D AdMSCs aggregates were injected into renal cortex immediately after induction of renal IR injury. In vivo data revealed that 3D aggregates enhanced the effects of AdMSCs in recovering function and structure after renal IR injury. Improved grafted AdMSCs were observed in kidney injected with 3D aggregates compared with AdMSCs cultured in 2D monolayer. Our results demonstrated that 3D AdMSCs aggregated produced by microwell enhanced the retention and therapeutic effects of AdMSCs for renal IR injury.

No MeSH data available.


Related in: MedlinePlus