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Encapsulated Whole Bone Marrow Cells Improve Survival in Wistar Rats after 90% Partial Hepatectomy.

Uribe-Cruz C, Kieling CO, López ML, Osvaldt A, Ochs de Muñoz G, da Silveira TR, Giugliani R, Matte U - Stem Cells Int (2015)

Bottom Line: Results.Conclusions.In addition, these cells favor apoptotic cell death and decrease necrosis, thus increasing survival.

View Article: PubMed Central - PubMed

Affiliation: Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, 90035-903 Porto Alegre, RS, Brazil ; Post-Graduation Program on Genetics and Molecular Biology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.

ABSTRACT
Background and Aims. The use of bone marrow cells has been suggested as an alternative treatment for acute liver failure. In this study, we investigate the effect of encapsulated whole bone marrow cells in a liver failure model. Methods. Encapsulated cells or empty capsules were implanted in rats submitted to 90% partial hepatectomy. The survival rate was assessed. Another group was euthanized at 6, 12, 24, 48, and 72 hours after hepatectomy to study expression of cytokines and growth factors. Results. Whole bone marrow group showed a higher than 10 days survival rate compared to empty capsules group. Gene expression related to early phase of liver regeneration at 6 hours after hepatectomy was decreased in encapsulated cells group, whereas genes related to regeneration were increased at 12, 24, and 48 hours. Whole bone marrow group showed lower regeneration rate at 72 hours and higher expression and activity of caspase 3. In contrast, lysosomal-β-glucuronidase activity was elevated in empty capsules group. Conclusions. The results show that encapsulated whole bone marrow cells reduce the expression of genes involved in liver regeneration and increase those responsible for ending hepatocyte division. In addition, these cells favor apoptotic cell death and decrease necrosis, thus increasing survival.

No MeSH data available.


Related in: MedlinePlus

Spontaneous survival according to therapeutic regimen in rats after 90% partial hepatectomy (PH). EC: empty capsules, WBM: whole bone marrow, and Cum: cumulative. Log rank = .002.
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fig1: Spontaneous survival according to therapeutic regimen in rats after 90% partial hepatectomy (PH). EC: empty capsules, WBM: whole bone marrow, and Cum: cumulative. Log rank = .002.

Mentions: Overall survival rate was observed during 10 days after hepatectomy. The survival rate was higher for the WBM group (63.6%) than for EC group (6.7%) (P = .002). Animals in WBM group died predominantly during the first three days, whereas in the other group deaths occurred over time after surgery (Figure 1). Therefore, to evaluate the effect of encapsulated WBM on the regeneration pathway the remaining analyses were performed in the first 72 hours after 90% PH.


Encapsulated Whole Bone Marrow Cells Improve Survival in Wistar Rats after 90% Partial Hepatectomy.

Uribe-Cruz C, Kieling CO, López ML, Osvaldt A, Ochs de Muñoz G, da Silveira TR, Giugliani R, Matte U - Stem Cells Int (2015)

Spontaneous survival according to therapeutic regimen in rats after 90% partial hepatectomy (PH). EC: empty capsules, WBM: whole bone marrow, and Cum: cumulative. Log rank = .002.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663362&req=5

fig1: Spontaneous survival according to therapeutic regimen in rats after 90% partial hepatectomy (PH). EC: empty capsules, WBM: whole bone marrow, and Cum: cumulative. Log rank = .002.
Mentions: Overall survival rate was observed during 10 days after hepatectomy. The survival rate was higher for the WBM group (63.6%) than for EC group (6.7%) (P = .002). Animals in WBM group died predominantly during the first three days, whereas in the other group deaths occurred over time after surgery (Figure 1). Therefore, to evaluate the effect of encapsulated WBM on the regeneration pathway the remaining analyses were performed in the first 72 hours after 90% PH.

Bottom Line: Results.Conclusions.In addition, these cells favor apoptotic cell death and decrease necrosis, thus increasing survival.

View Article: PubMed Central - PubMed

Affiliation: Gene Therapy Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, 90035-903 Porto Alegre, RS, Brazil ; Post-Graduation Program on Genetics and Molecular Biology, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.

ABSTRACT
Background and Aims. The use of bone marrow cells has been suggested as an alternative treatment for acute liver failure. In this study, we investigate the effect of encapsulated whole bone marrow cells in a liver failure model. Methods. Encapsulated cells or empty capsules were implanted in rats submitted to 90% partial hepatectomy. The survival rate was assessed. Another group was euthanized at 6, 12, 24, 48, and 72 hours after hepatectomy to study expression of cytokines and growth factors. Results. Whole bone marrow group showed a higher than 10 days survival rate compared to empty capsules group. Gene expression related to early phase of liver regeneration at 6 hours after hepatectomy was decreased in encapsulated cells group, whereas genes related to regeneration were increased at 12, 24, and 48 hours. Whole bone marrow group showed lower regeneration rate at 72 hours and higher expression and activity of caspase 3. In contrast, lysosomal-β-glucuronidase activity was elevated in empty capsules group. Conclusions. The results show that encapsulated whole bone marrow cells reduce the expression of genes involved in liver regeneration and increase those responsible for ending hepatocyte division. In addition, these cells favor apoptotic cell death and decrease necrosis, thus increasing survival.

No MeSH data available.


Related in: MedlinePlus