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Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain.

Horii-Hayashi N, Sasagawa T, Matsunaga W, Nishi M - Neural Plast. (2015)

Bottom Line: In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala.Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms.The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.

ABSTRACT
Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In adult mammals, CSPGs form the specialized ECM structure perineuronal nets (PNNs) that surround somata and dendrites of certain types of neurons. PNNs restrict synaptic plasticity and regulate the closure of critical periods. Although previous studies have examined the starting period of PNN formation, focusing on primary sensory cortices, there are no systematic studies at the whole brain level. Here, we examined the starting period of PNN formation in male mice ranging in age from postnatal day 3 to week 11, mainly focusing on several cortical areas, limbic structures, hypothalamus, and brain stem, using lectin histochemistry with Wisteria floribunda agglutinin (WFA). Results showed that early PNN formation was observed in several reticular formations of the brain stem related to the cranial nerves and primary somatosensory cortices. In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala. Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms. The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

No MeSH data available.


Related in: MedlinePlus

Structural variety of CSPG-contained ECM structures. (a–l) Fluorescent triple labeling of WFA (green), Nissl (red, neuron marker), and DAPI (blue, nucleus marker) at 11 w. Arrows indicate WFA signals around dendrites. The sign of plus (+) or minus (−) below images indicates the presence or absence of WFA reactivity around somata and dendrites. WFA signals in the Cx (a), hippocampal CA1 (b), mRt (c), RN (d), Ve (e), Gi (f), Sp5 (g), and LH (h) were observed around both somata and dendrites, while those of the Rt (i), MA (j), VMH (k), and Arc (l) did not show clear reactivity surrounding dendrites. Arc: arcuate nucleus; Gi: gigantocellular nucleus; LH: lateral hypothalamus; MA: medial amygdaloid nucleus; mRt: mesencephalic reticular formation; RN: red nucleus; Rt: reticular thalamic nucleus; S1BF: barrel field of the primary somatosensory cortex; Sp5: spinal trigeminal nucleus; Ve: vestibular nucleus; VMH: ventromedial hypothalamic nucleus. Scale bar = 100 μm (a–l).
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fig6: Structural variety of CSPG-contained ECM structures. (a–l) Fluorescent triple labeling of WFA (green), Nissl (red, neuron marker), and DAPI (blue, nucleus marker) at 11 w. Arrows indicate WFA signals around dendrites. The sign of plus (+) or minus (−) below images indicates the presence or absence of WFA reactivity around somata and dendrites. WFA signals in the Cx (a), hippocampal CA1 (b), mRt (c), RN (d), Ve (e), Gi (f), Sp5 (g), and LH (h) were observed around both somata and dendrites, while those of the Rt (i), MA (j), VMH (k), and Arc (l) did not show clear reactivity surrounding dendrites. Arc: arcuate nucleus; Gi: gigantocellular nucleus; LH: lateral hypothalamus; MA: medial amygdaloid nucleus; mRt: mesencephalic reticular formation; RN: red nucleus; Rt: reticular thalamic nucleus; S1BF: barrel field of the primary somatosensory cortex; Sp5: spinal trigeminal nucleus; Ve: vestibular nucleus; VMH: ventromedial hypothalamic nucleus. Scale bar = 100 μm (a–l).

Mentions: WFA-labeled ECM structures at 11 w were examined by fluorescent labeling with WFA, Nissl (neuron marker), and DAPI (nuclear marker). PNN-like WFA reactivity that was condensed around both cell bodies and dendrites was observed in many cortical, limbic, and brain stem areas, including the S1BF (Figure 6(a)), hippocampal CA1 (Figure 6(b)) and CA3 (data not shown), BLA (data not shown), lateral septum (data not shown), mesencephalic reticular formation (mRt, Figure 6(c)), red nucleus (RN, Figure 6(d)), vestibular nucleus (Ve, Figure 6(e)), Gi (Figure 6(f)), and spinal trigeminal nucleus (Sp5, Figure 6(g)). In the hypothalamus, the typical PNN structure was observed in the LH (Figure 6(h)) and LPO (data not shown). On the other hand, WFA reactivity in the reticular thalamic nucleus (Rt, Figure 6(i)), MA (Figure 6(j)), VMH (Figure 6(k)), and Arc (Figure 6(l)) was observed to surround cell bodies but not dendrites. WFA reactivity in the MA (Figure 6(j)) and VMH (Figure 6(k)) was particularly more diffuse and showed a more loosely organized accumulation around cell bodies. These results suggested structural variety of CSPG-contained ECM in the adult brain.


Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain.

Horii-Hayashi N, Sasagawa T, Matsunaga W, Nishi M - Neural Plast. (2015)

Structural variety of CSPG-contained ECM structures. (a–l) Fluorescent triple labeling of WFA (green), Nissl (red, neuron marker), and DAPI (blue, nucleus marker) at 11 w. Arrows indicate WFA signals around dendrites. The sign of plus (+) or minus (−) below images indicates the presence or absence of WFA reactivity around somata and dendrites. WFA signals in the Cx (a), hippocampal CA1 (b), mRt (c), RN (d), Ve (e), Gi (f), Sp5 (g), and LH (h) were observed around both somata and dendrites, while those of the Rt (i), MA (j), VMH (k), and Arc (l) did not show clear reactivity surrounding dendrites. Arc: arcuate nucleus; Gi: gigantocellular nucleus; LH: lateral hypothalamus; MA: medial amygdaloid nucleus; mRt: mesencephalic reticular formation; RN: red nucleus; Rt: reticular thalamic nucleus; S1BF: barrel field of the primary somatosensory cortex; Sp5: spinal trigeminal nucleus; Ve: vestibular nucleus; VMH: ventromedial hypothalamic nucleus. Scale bar = 100 μm (a–l).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig6: Structural variety of CSPG-contained ECM structures. (a–l) Fluorescent triple labeling of WFA (green), Nissl (red, neuron marker), and DAPI (blue, nucleus marker) at 11 w. Arrows indicate WFA signals around dendrites. The sign of plus (+) or minus (−) below images indicates the presence or absence of WFA reactivity around somata and dendrites. WFA signals in the Cx (a), hippocampal CA1 (b), mRt (c), RN (d), Ve (e), Gi (f), Sp5 (g), and LH (h) were observed around both somata and dendrites, while those of the Rt (i), MA (j), VMH (k), and Arc (l) did not show clear reactivity surrounding dendrites. Arc: arcuate nucleus; Gi: gigantocellular nucleus; LH: lateral hypothalamus; MA: medial amygdaloid nucleus; mRt: mesencephalic reticular formation; RN: red nucleus; Rt: reticular thalamic nucleus; S1BF: barrel field of the primary somatosensory cortex; Sp5: spinal trigeminal nucleus; Ve: vestibular nucleus; VMH: ventromedial hypothalamic nucleus. Scale bar = 100 μm (a–l).
Mentions: WFA-labeled ECM structures at 11 w were examined by fluorescent labeling with WFA, Nissl (neuron marker), and DAPI (nuclear marker). PNN-like WFA reactivity that was condensed around both cell bodies and dendrites was observed in many cortical, limbic, and brain stem areas, including the S1BF (Figure 6(a)), hippocampal CA1 (Figure 6(b)) and CA3 (data not shown), BLA (data not shown), lateral septum (data not shown), mesencephalic reticular formation (mRt, Figure 6(c)), red nucleus (RN, Figure 6(d)), vestibular nucleus (Ve, Figure 6(e)), Gi (Figure 6(f)), and spinal trigeminal nucleus (Sp5, Figure 6(g)). In the hypothalamus, the typical PNN structure was observed in the LH (Figure 6(h)) and LPO (data not shown). On the other hand, WFA reactivity in the reticular thalamic nucleus (Rt, Figure 6(i)), MA (Figure 6(j)), VMH (Figure 6(k)), and Arc (Figure 6(l)) was observed to surround cell bodies but not dendrites. WFA reactivity in the MA (Figure 6(j)) and VMH (Figure 6(k)) was particularly more diffuse and showed a more loosely organized accumulation around cell bodies. These results suggested structural variety of CSPG-contained ECM in the adult brain.

Bottom Line: In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala.Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms.The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.

ABSTRACT
Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In adult mammals, CSPGs form the specialized ECM structure perineuronal nets (PNNs) that surround somata and dendrites of certain types of neurons. PNNs restrict synaptic plasticity and regulate the closure of critical periods. Although previous studies have examined the starting period of PNN formation, focusing on primary sensory cortices, there are no systematic studies at the whole brain level. Here, we examined the starting period of PNN formation in male mice ranging in age from postnatal day 3 to week 11, mainly focusing on several cortical areas, limbic structures, hypothalamus, and brain stem, using lectin histochemistry with Wisteria floribunda agglutinin (WFA). Results showed that early PNN formation was observed in several reticular formations of the brain stem related to the cranial nerves and primary somatosensory cortices. In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala. Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms. The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

No MeSH data available.


Related in: MedlinePlus