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Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain.

Horii-Hayashi N, Sasagawa T, Matsunaga W, Nishi M - Neural Plast. (2015)

Bottom Line: In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala.Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms.The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.

ABSTRACT
Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In adult mammals, CSPGs form the specialized ECM structure perineuronal nets (PNNs) that surround somata and dendrites of certain types of neurons. PNNs restrict synaptic plasticity and regulate the closure of critical periods. Although previous studies have examined the starting period of PNN formation, focusing on primary sensory cortices, there are no systematic studies at the whole brain level. Here, we examined the starting period of PNN formation in male mice ranging in age from postnatal day 3 to week 11, mainly focusing on several cortical areas, limbic structures, hypothalamus, and brain stem, using lectin histochemistry with Wisteria floribunda agglutinin (WFA). Results showed that early PNN formation was observed in several reticular formations of the brain stem related to the cranial nerves and primary somatosensory cortices. In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala. Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms. The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

No MeSH data available.


WFA-stained ECM in the developing amygdala. (a–h) WFA-labeled images in the BLA (a–d) and MA (e–h) at P14 (a, e), P21 (b, f), 5 w (c, g), and 11 w (d, h). In both regions, WFA reactivity was observed from P21 onward. (i–n) Higher magnification images of the BLA (i–k) and MA (l–n) at P21 (i, l), 5 w (j, m), and 11 w (k, n). Ambiguous PNN-like staining was observed in the BLA at P21 and 5 w, which surrounded both cell bodies and dendrites at 11 w. Diffuse neuropil-like staining was continuously observed in the MA at all stages shown and a loosely accumulated WFA reactivity around particular cell bodies was observed at 11 w. BLA: basolateral amygdaloid nucleus; MA: medial amygdaloid nucleus. Scale bars = 500 (a–h) and 50 (i–n) μm.
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fig3: WFA-stained ECM in the developing amygdala. (a–h) WFA-labeled images in the BLA (a–d) and MA (e–h) at P14 (a, e), P21 (b, f), 5 w (c, g), and 11 w (d, h). In both regions, WFA reactivity was observed from P21 onward. (i–n) Higher magnification images of the BLA (i–k) and MA (l–n) at P21 (i, l), 5 w (j, m), and 11 w (k, n). Ambiguous PNN-like staining was observed in the BLA at P21 and 5 w, which surrounded both cell bodies and dendrites at 11 w. Diffuse neuropil-like staining was continuously observed in the MA at all stages shown and a loosely accumulated WFA reactivity around particular cell bodies was observed at 11 w. BLA: basolateral amygdaloid nucleus; MA: medial amygdaloid nucleus. Scale bars = 500 (a–h) and 50 (i–n) μm.

Mentions: In the amygdala, WFA reactivity was hardly observed by P14 in both the basolateral amygdaloid nucleus (BLA) (Figure 3(a)) and medial amygdaloid nucleus (MA) (Figure 3(e)). At P21, WFA reactivity in both subdivisions became detectable (Figures 3(b) and 3(f)). From 5 w onward, WFA reactivity was clearly observed in the BLA (Figures 3(c) and 3(d)) and MA (Figures 3(g) and 3(h)). High-power images further showed that an immature PNN-like form was observed in the BLA at P21 (Figure 3(i)), which gradually and clearly surrounded both cell bodies and dendrites from 5 w (Figure 3(j)) to 11 w (Figure 3(k)). However, in the MA, no clear WFA reactivity was observed to surround cell bodies and dendrites at P21 (Figure 3(l)) and 5 w (Figure 3(m)). At 11 w, WFA reactivity loosely accumulated around particular cell bodies but not around dendrites (Figure 3(n)).


Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain.

Horii-Hayashi N, Sasagawa T, Matsunaga W, Nishi M - Neural Plast. (2015)

WFA-stained ECM in the developing amygdala. (a–h) WFA-labeled images in the BLA (a–d) and MA (e–h) at P14 (a, e), P21 (b, f), 5 w (c, g), and 11 w (d, h). In both regions, WFA reactivity was observed from P21 onward. (i–n) Higher magnification images of the BLA (i–k) and MA (l–n) at P21 (i, l), 5 w (j, m), and 11 w (k, n). Ambiguous PNN-like staining was observed in the BLA at P21 and 5 w, which surrounded both cell bodies and dendrites at 11 w. Diffuse neuropil-like staining was continuously observed in the MA at all stages shown and a loosely accumulated WFA reactivity around particular cell bodies was observed at 11 w. BLA: basolateral amygdaloid nucleus; MA: medial amygdaloid nucleus. Scale bars = 500 (a–h) and 50 (i–n) μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: WFA-stained ECM in the developing amygdala. (a–h) WFA-labeled images in the BLA (a–d) and MA (e–h) at P14 (a, e), P21 (b, f), 5 w (c, g), and 11 w (d, h). In both regions, WFA reactivity was observed from P21 onward. (i–n) Higher magnification images of the BLA (i–k) and MA (l–n) at P21 (i, l), 5 w (j, m), and 11 w (k, n). Ambiguous PNN-like staining was observed in the BLA at P21 and 5 w, which surrounded both cell bodies and dendrites at 11 w. Diffuse neuropil-like staining was continuously observed in the MA at all stages shown and a loosely accumulated WFA reactivity around particular cell bodies was observed at 11 w. BLA: basolateral amygdaloid nucleus; MA: medial amygdaloid nucleus. Scale bars = 500 (a–h) and 50 (i–n) μm.
Mentions: In the amygdala, WFA reactivity was hardly observed by P14 in both the basolateral amygdaloid nucleus (BLA) (Figure 3(a)) and medial amygdaloid nucleus (MA) (Figure 3(e)). At P21, WFA reactivity in both subdivisions became detectable (Figures 3(b) and 3(f)). From 5 w onward, WFA reactivity was clearly observed in the BLA (Figures 3(c) and 3(d)) and MA (Figures 3(g) and 3(h)). High-power images further showed that an immature PNN-like form was observed in the BLA at P21 (Figure 3(i)), which gradually and clearly surrounded both cell bodies and dendrites from 5 w (Figure 3(j)) to 11 w (Figure 3(k)). However, in the MA, no clear WFA reactivity was observed to surround cell bodies and dendrites at P21 (Figure 3(l)) and 5 w (Figure 3(m)). At 11 w, WFA reactivity loosely accumulated around particular cell bodies but not around dendrites (Figure 3(n)).

Bottom Line: In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala.Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms.The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.

ABSTRACT
Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In adult mammals, CSPGs form the specialized ECM structure perineuronal nets (PNNs) that surround somata and dendrites of certain types of neurons. PNNs restrict synaptic plasticity and regulate the closure of critical periods. Although previous studies have examined the starting period of PNN formation, focusing on primary sensory cortices, there are no systematic studies at the whole brain level. Here, we examined the starting period of PNN formation in male mice ranging in age from postnatal day 3 to week 11, mainly focusing on several cortical areas, limbic structures, hypothalamus, and brain stem, using lectin histochemistry with Wisteria floribunda agglutinin (WFA). Results showed that early PNN formation was observed in several reticular formations of the brain stem related to the cranial nerves and primary somatosensory cortices. In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala. Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms. The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

No MeSH data available.