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Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain.

Horii-Hayashi N, Sasagawa T, Matsunaga W, Nishi M - Neural Plast. (2015)

Bottom Line: In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala.Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms.The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.

ABSTRACT
Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In adult mammals, CSPGs form the specialized ECM structure perineuronal nets (PNNs) that surround somata and dendrites of certain types of neurons. PNNs restrict synaptic plasticity and regulate the closure of critical periods. Although previous studies have examined the starting period of PNN formation, focusing on primary sensory cortices, there are no systematic studies at the whole brain level. Here, we examined the starting period of PNN formation in male mice ranging in age from postnatal day 3 to week 11, mainly focusing on several cortical areas, limbic structures, hypothalamus, and brain stem, using lectin histochemistry with Wisteria floribunda agglutinin (WFA). Results showed that early PNN formation was observed in several reticular formations of the brain stem related to the cranial nerves and primary somatosensory cortices. In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala. Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms. The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

No MeSH data available.


WFA-stained ECM in the developing hippocampus. (a–e) WFA-labeled images at P3 (a), P7 (B), P14 (c), P21 (d), and 5 w (e). PNN-like WFA reactivity was observed from P14 onward. (f–m) Higher magnification images of the CA1 (f–i) and CA2 (j–m) at P14 (f, j), P21 (g, k), 5 w (h, l), and 11 w (i, m). Diffuse PNN-like staining was observed in the CA1 at P14, which gradually surrounded cell bodies and dendrites from P21 onward. WFA reactivity in the CA2 from P21 onward was strong and showed a complex staining manner. Cx: cerebral cortex. Scale bars = 500 (a–e) and 50 (f–m) μm.
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fig2: WFA-stained ECM in the developing hippocampus. (a–e) WFA-labeled images at P3 (a), P7 (B), P14 (c), P21 (d), and 5 w (e). PNN-like WFA reactivity was observed from P14 onward. (f–m) Higher magnification images of the CA1 (f–i) and CA2 (j–m) at P14 (f, j), P21 (g, k), 5 w (h, l), and 11 w (i, m). Diffuse PNN-like staining was observed in the CA1 at P14, which gradually surrounded cell bodies and dendrites from P21 onward. WFA reactivity in the CA2 from P21 onward was strong and showed a complex staining manner. Cx: cerebral cortex. Scale bars = 500 (a–e) and 50 (f–m) μm.

Mentions: In the hippocampal CA1, PNN-like WFA labeling could not be observed at P3 (Figure 2(a)) and P7 (Figure 2(b)), while it could be detected at P14 (Figure 2(c)). Similarly, clear WFA labeling was first observed in the hippocampal CA2 at P14 (Figure 2(c)). These labels became stronger and clearer at P21 (Figure 2(d)) and 5 w (Figure 2(e)). Higher magnification views confirmed PNN-like labeling in the CA1 at P14 (Figure 2(f)). From P21 onward (Figures 2(g)–2(i)), WFA reactivity clearly surrounded both cell bodies and dendrites in the CA1. WFA reactivity in the CA2 at P14 showed PNN-like labeling (Figure 2(j)), while, from P21 onward, the border of individual PNN structures was difficult to identify (Figures 2(k)–2(m)). Such a distinctive staining pattern in the CA2 is consistent with what has been previously reported [10, 20].


Development and Structural Variety of the Chondroitin Sulfate Proteoglycans-Contained Extracellular Matrix in the Mouse Brain.

Horii-Hayashi N, Sasagawa T, Matsunaga W, Nishi M - Neural Plast. (2015)

WFA-stained ECM in the developing hippocampus. (a–e) WFA-labeled images at P3 (a), P7 (B), P14 (c), P21 (d), and 5 w (e). PNN-like WFA reactivity was observed from P14 onward. (f–m) Higher magnification images of the CA1 (f–i) and CA2 (j–m) at P14 (f, j), P21 (g, k), 5 w (h, l), and 11 w (i, m). Diffuse PNN-like staining was observed in the CA1 at P14, which gradually surrounded cell bodies and dendrites from P21 onward. WFA reactivity in the CA2 from P21 onward was strong and showed a complex staining manner. Cx: cerebral cortex. Scale bars = 500 (a–e) and 50 (f–m) μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig2: WFA-stained ECM in the developing hippocampus. (a–e) WFA-labeled images at P3 (a), P7 (B), P14 (c), P21 (d), and 5 w (e). PNN-like WFA reactivity was observed from P14 onward. (f–m) Higher magnification images of the CA1 (f–i) and CA2 (j–m) at P14 (f, j), P21 (g, k), 5 w (h, l), and 11 w (i, m). Diffuse PNN-like staining was observed in the CA1 at P14, which gradually surrounded cell bodies and dendrites from P21 onward. WFA reactivity in the CA2 from P21 onward was strong and showed a complex staining manner. Cx: cerebral cortex. Scale bars = 500 (a–e) and 50 (f–m) μm.
Mentions: In the hippocampal CA1, PNN-like WFA labeling could not be observed at P3 (Figure 2(a)) and P7 (Figure 2(b)), while it could be detected at P14 (Figure 2(c)). Similarly, clear WFA labeling was first observed in the hippocampal CA2 at P14 (Figure 2(c)). These labels became stronger and clearer at P21 (Figure 2(d)) and 5 w (Figure 2(e)). Higher magnification views confirmed PNN-like labeling in the CA1 at P14 (Figure 2(f)). From P21 onward (Figures 2(g)–2(i)), WFA reactivity clearly surrounded both cell bodies and dendrites in the CA1. WFA reactivity in the CA2 at P14 showed PNN-like labeling (Figure 2(j)), while, from P21 onward, the border of individual PNN structures was difficult to identify (Figures 2(k)–2(m)). Such a distinctive staining pattern in the CA2 is consistent with what has been previously reported [10, 20].

Bottom Line: In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala.Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms.The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.

ABSTRACT
Chondroitin sulfate proteoglycans (CSPGs) are major components of the extracellular matrix (ECM) in the brain. In adult mammals, CSPGs form the specialized ECM structure perineuronal nets (PNNs) that surround somata and dendrites of certain types of neurons. PNNs restrict synaptic plasticity and regulate the closure of critical periods. Although previous studies have examined the starting period of PNN formation, focusing on primary sensory cortices, there are no systematic studies at the whole brain level. Here, we examined the starting period of PNN formation in male mice ranging in age from postnatal day 3 to week 11, mainly focusing on several cortical areas, limbic structures, hypothalamus, and brain stem, using lectin histochemistry with Wisteria floribunda agglutinin (WFA). Results showed that early PNN formation was observed in several reticular formations of the brain stem related to the cranial nerves and primary somatosensory cortices. In the limbic system, PNN formation in the hippocampus started earlier than that of the amygdala. Furthermore, in the medial amygdaloid nucleus and some hypothalamic regions, WFA labeling did not show typical PNN-like forms. The present study suggests spatiotemporal differences at the beginning of PNN formation and a structural variety of CSPG-contained ECM in the brain.

No MeSH data available.