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Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence labeling of NF200 ((a), (b), and (c)) and GFAP ((e), (f), and (g)) in sagittal sections of the injured spinal cords from each group on day 35 after cell transplantation. Double labeling for NF200/GFAP (green) and BMSCs (red). Magnification, ×400 ((a)–(c), (e)–(g)). Scale bar, 50 μm ((a)–(c), (e)–(g)). Integrated optical density (IOD) bar graphs showing NF200 (g) and GFAP (h) expression in each group. All microscopic images were captured under identical conditions. The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
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fig8: Immunofluorescence labeling of NF200 ((a), (b), and (c)) and GFAP ((e), (f), and (g)) in sagittal sections of the injured spinal cords from each group on day 35 after cell transplantation. Double labeling for NF200/GFAP (green) and BMSCs (red). Magnification, ×400 ((a)–(c), (e)–(g)). Scale bar, 50 μm ((a)–(c), (e)–(g)). Integrated optical density (IOD) bar graphs showing NF200 (g) and GFAP (h) expression in each group. All microscopic images were captured under identical conditions. The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.

Mentions: NF200 is a NF protein that is found in axons under normal conditions [16]. Increased expression of NF200 indicates axon regeneration in the injured spinal cord. In contrast, GFAP is expressed primarily in astrocytes, and a reduction of its expression indicates glial scar inhibition in the injured spinal cord. As shown in Figure 8, the level of NF200 expression in the M-NT group was significantly increased compared with that in the other two groups, whereas the GFAP expression level in the M-NT group was significantly decreased compared with that in the other two groups. Moreover, the NF200 expression level in the NT3 group was higher than in the BMSC group, whereas the GFAP expression level in the NT3 group was lower than in the BMSC group.


Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Immunofluorescence labeling of NF200 ((a), (b), and (c)) and GFAP ((e), (f), and (g)) in sagittal sections of the injured spinal cords from each group on day 35 after cell transplantation. Double labeling for NF200/GFAP (green) and BMSCs (red). Magnification, ×400 ((a)–(c), (e)–(g)). Scale bar, 50 μm ((a)–(c), (e)–(g)). Integrated optical density (IOD) bar graphs showing NF200 (g) and GFAP (h) expression in each group. All microscopic images were captured under identical conditions. The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig8: Immunofluorescence labeling of NF200 ((a), (b), and (c)) and GFAP ((e), (f), and (g)) in sagittal sections of the injured spinal cords from each group on day 35 after cell transplantation. Double labeling for NF200/GFAP (green) and BMSCs (red). Magnification, ×400 ((a)–(c), (e)–(g)). Scale bar, 50 μm ((a)–(c), (e)–(g)). Integrated optical density (IOD) bar graphs showing NF200 (g) and GFAP (h) expression in each group. All microscopic images were captured under identical conditions. The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
Mentions: NF200 is a NF protein that is found in axons under normal conditions [16]. Increased expression of NF200 indicates axon regeneration in the injured spinal cord. In contrast, GFAP is expressed primarily in astrocytes, and a reduction of its expression indicates glial scar inhibition in the injured spinal cord. As shown in Figure 8, the level of NF200 expression in the M-NT group was significantly increased compared with that in the other two groups, whereas the GFAP expression level in the M-NT group was significantly decreased compared with that in the other two groups. Moreover, the NF200 expression level in the NT3 group was higher than in the BMSC group, whereas the GFAP expression level in the NT3 group was lower than in the BMSC group.

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus