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Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus

Western blot detection of NT3 protein expression in the injured spinal cords on day 35 after cell transplantation in each group (a). Ratio of the lane density of the NT3 protein to the lane density of the β-actin protein in each group (b). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
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fig7: Western blot detection of NT3 protein expression in the injured spinal cords on day 35 after cell transplantation in each group (a). Ratio of the lane density of the NT3 protein to the lane density of the β-actin protein in each group (b). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.

Mentions: On day 35 after cell transplantation, NT3 protein expression was investigated through western blot analysis (Figure 7). Although NT3 protein overexpression was observed in both the M-NT3 group and the NT3 group, the NT3 protein level in the M-NT3 group was significantly higher than in the NT3 group. In addition, the NT3 protein level in the BMSC group was significantly lower than in the NT3 group. The magnetic targeting system significantly enhanced NT3 protein expression in the injured spinal cord lesions.


Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Western blot detection of NT3 protein expression in the injured spinal cords on day 35 after cell transplantation in each group (a). Ratio of the lane density of the NT3 protein to the lane density of the β-actin protein in each group (b). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663356&req=5

fig7: Western blot detection of NT3 protein expression in the injured spinal cords on day 35 after cell transplantation in each group (a). Ratio of the lane density of the NT3 protein to the lane density of the β-actin protein in each group (b). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
Mentions: On day 35 after cell transplantation, NT3 protein expression was investigated through western blot analysis (Figure 7). Although NT3 protein overexpression was observed in both the M-NT3 group and the NT3 group, the NT3 protein level in the M-NT3 group was significantly higher than in the NT3 group. In addition, the NT3 protein level in the BMSC group was significantly lower than in the NT3 group. The magnetic targeting system significantly enhanced NT3 protein expression in the injured spinal cord lesions.

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus