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Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus

The cystic cavity area of the injured spinal cords on day 35 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). Magnification, ×40 ((a), (b), and (c)). Scale bar, 200 μm ((a), (b), and (c)). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
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fig6: The cystic cavity area of the injured spinal cords on day 35 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). Magnification, ×40 ((a), (b), and (c)). Scale bar, 200 μm ((a), (b), and (c)). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.

Mentions: At 7 weeks after the spinal cord was injured, the mean values of the cystic cavity area in the BMSC, NT3, and M-NT3 groups were 0.64 ± 0.14 mm2, 0.51 ± 0.11 mm2, and 0.39 ± 0.10 mm2, respectively. Statistically significant differences were observed among the three groups (p < 0.05, Figure 6), with the cystic cavity area in the M-NT3 group being the smallest.


Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

The cystic cavity area of the injured spinal cords on day 35 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). Magnification, ×40 ((a), (b), and (c)). Scale bar, 200 μm ((a), (b), and (c)). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig6: The cystic cavity area of the injured spinal cords on day 35 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). Magnification, ×40 ((a), (b), and (c)). Scale bar, 200 μm ((a), (b), and (c)). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
Mentions: At 7 weeks after the spinal cord was injured, the mean values of the cystic cavity area in the BMSC, NT3, and M-NT3 groups were 0.64 ± 0.14 mm2, 0.51 ± 0.11 mm2, and 0.39 ± 0.10 mm2, respectively. Statistically significant differences were observed among the three groups (p < 0.05, Figure 6), with the cystic cavity area in the M-NT3 group being the smallest.

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus