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Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus

The BBB scores of the hind-limb locomotor functions in each group on days 1, 3, 7, 14, 21, 28, and 35 after cell transplantation ((a), (b)). The data, which are presented as the means ± SD (n = 12), were analyzed using repeated measures ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
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fig4: The BBB scores of the hind-limb locomotor functions in each group on days 1, 3, 7, 14, 21, 28, and 35 after cell transplantation ((a), (b)). The data, which are presented as the means ± SD (n = 12), were analyzed using repeated measures ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.

Mentions: The hind-limb locomotor functions in each group were assessed using the BBB scores on days 1, 3, 7, 14, 21, 28, and 35 after cell transplantation (Figure 4). On days 1 and 3 after cell transplantation, the BBB scores of the M-NT3 group did not improve significantly compared with those of the other two groups. However, the BBB scores of the M-NT3 group were the highest among the three groups on days 7, 14, 21, 28, and 35 after cell transplantation, which indicated that hind-limb locomotor functional recovery was enhanced and accelerated in the M-NT3 group compared with the other two groups. The BBB scores of the NT3 group were also significantly higher than those of the BMSC group on days 7, 14, 21, 28, and 35 after cell transplantation. Moreover, significant differences (p < 0.05) were observed among the three groups at these time points.


Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

The BBB scores of the hind-limb locomotor functions in each group on days 1, 3, 7, 14, 21, 28, and 35 after cell transplantation ((a), (b)). The data, which are presented as the means ± SD (n = 12), were analyzed using repeated measures ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663356&req=5

fig4: The BBB scores of the hind-limb locomotor functions in each group on days 1, 3, 7, 14, 21, 28, and 35 after cell transplantation ((a), (b)). The data, which are presented as the means ± SD (n = 12), were analyzed using repeated measures ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
Mentions: The hind-limb locomotor functions in each group were assessed using the BBB scores on days 1, 3, 7, 14, 21, 28, and 35 after cell transplantation (Figure 4). On days 1 and 3 after cell transplantation, the BBB scores of the M-NT3 group did not improve significantly compared with those of the other two groups. However, the BBB scores of the M-NT3 group were the highest among the three groups on days 7, 14, 21, 28, and 35 after cell transplantation, which indicated that hind-limb locomotor functional recovery was enhanced and accelerated in the M-NT3 group compared with the other two groups. The BBB scores of the NT3 group were also significantly higher than those of the BMSC group on days 7, 14, 21, 28, and 35 after cell transplantation. Moreover, significant differences (p < 0.05) were observed among the three groups at these time points.

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus