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Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus

T2∗-weighted MR images of the injured spinal cord on day 1 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). A bar graph showing the SNRs in the injured spinal cord MR images from each group (d). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
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fig3: T2∗-weighted MR images of the injured spinal cord on day 1 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). A bar graph showing the SNRs in the injured spinal cord MR images from each group (d). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.

Mentions: The SI was obtained using T2∗-weighted gradient-echo sequences in the injured spinal cord because T2∗-weighted imaging is sensitive to SPIO (Figure 3). The injured spinal cord SIs of the M-NT3 and NT3 groups decreased after transplantation, whereas no apparent change in the SI was observed in the BMSC group. Analysis of the SNR further revealed that the injured spinal cord SNRs of the M-NT3 group were significantly decreased compared with those of the NT3 group, and the SNRs for the injured spinal cords of the NT3 group were significantly decreased compared with those of the BMSC group.


Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

T2∗-weighted MR images of the injured spinal cord on day 1 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). A bar graph showing the SNRs in the injured spinal cord MR images from each group (d). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4663356&req=5

fig3: T2∗-weighted MR images of the injured spinal cord on day 1 after cell transplantation in the BMSC group (a), NT3 group (b), and M-NT3 group (c). A bar graph showing the SNRs in the injured spinal cord MR images from each group (d). The data, which are presented as the means ± SD (n = 12), were analyzed using one-way ANOVA. ∗p < 0.05 versus the BMSC group, #p < 0.05 versus the NT3 group.
Mentions: The SI was obtained using T2∗-weighted gradient-echo sequences in the injured spinal cord because T2∗-weighted imaging is sensitive to SPIO (Figure 3). The injured spinal cord SIs of the M-NT3 and NT3 groups decreased after transplantation, whereas no apparent change in the SI was observed in the BMSC group. Analysis of the SNR further revealed that the injured spinal cord SNRs of the M-NT3 group were significantly decreased compared with those of the NT3 group, and the SNRs for the injured spinal cords of the NT3 group were significantly decreased compared with those of the BMSC group.

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus