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Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus

Stable transfection of the NT3-DsRed gene into BMSCs using a lentivirus ((a), (b)) and Q-PCT detection of NT3 mRNA ((c), (d)). (a) Before transfection. (b) After transfection. (c) NT3 mRNA Q-PCR melting curve. (d) The normalized fold change in NT3 mRNA expression (2−ΔΔCT). Magnification, ×100 ((a), (b)). Scale bar, 100 μm ((a), (b)).
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fig1: Stable transfection of the NT3-DsRed gene into BMSCs using a lentivirus ((a), (b)) and Q-PCT detection of NT3 mRNA ((c), (d)). (a) Before transfection. (b) After transfection. (c) NT3 mRNA Q-PCR melting curve. (d) The normalized fold change in NT3 mRNA expression (2−ΔΔCT). Magnification, ×100 ((a), (b)). Scale bar, 100 μm ((a), (b)).

Mentions: After puromycin screening, red fluorescence was identified in the cytoplasm of >95% of the NT3-DsRed BMSCs and >99% of the DsRed BMSCs (Figure 1), which indicated a high transfection efficiency for BMSCs in the present study.


Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury.

Zhang RP, Wang LJ, He S, Xie J, Li JD - Stem Cells Int (2015)

Stable transfection of the NT3-DsRed gene into BMSCs using a lentivirus ((a), (b)) and Q-PCT detection of NT3 mRNA ((c), (d)). (a) Before transfection. (b) After transfection. (c) NT3 mRNA Q-PCR melting curve. (d) The normalized fold change in NT3 mRNA expression (2−ΔΔCT). Magnification, ×100 ((a), (b)). Scale bar, 100 μm ((a), (b)).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663356&req=5

fig1: Stable transfection of the NT3-DsRed gene into BMSCs using a lentivirus ((a), (b)) and Q-PCT detection of NT3 mRNA ((c), (d)). (a) Before transfection. (b) After transfection. (c) NT3 mRNA Q-PCR melting curve. (d) The normalized fold change in NT3 mRNA expression (2−ΔΔCT). Magnification, ×100 ((a), (b)). Scale bar, 100 μm ((a), (b)).
Mentions: After puromycin screening, red fluorescence was identified in the cytoplasm of >95% of the NT3-DsRed BMSCs and >99% of the DsRed BMSCs (Figure 1), which indicated a high transfection efficiency for BMSCs in the present study.

Bottom Line: Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality.In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system.Our results suggest that this composite strategy could be promising for clinical applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.

ABSTRACT
Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplanting NT3 gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting of NT3 gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplanting NT3 gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

No MeSH data available.


Related in: MedlinePlus