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Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats.

Chou WC, Kao MC, Yue CT, Tsai PS, Huang CJ - Mediators Inflamm. (2015)

Bottom Line: Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group.Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.).Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 289 Jianguo Road, Xindian District, New Taipei City 23142, Taiwan ; School of Medicine, Tzu Chi University, No. 701, Sec. 3, Zhongyang Road, Hualien 97004, Taiwan.

ABSTRACT
Reperfusion of ischemic limbs can induce inflammation and subsequently cause acute lung injury. Caffeine, a widely used psychostimulant, possesses potent anti-inflammatory capacity. We elucidated whether caffeine can mitigate lung inflammation caused by ischemia-reperfusion (IR) of the lower limbs. Adult male Sprague-Dawley rats were randomly allocated to receive IR, IR plus caffeine (IR + Caf group), sham-operation (Sham), or sham plus caffeine (n = 12 in each group). To induce IR, lower limbs were bilaterally tied by rubber bands high around each thigh for 3 hours followed by reperfusion for 3 hours. Caffeine (50 mg/kg, intraperitoneal injection) was administered immediately after reperfusion. Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group. Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.). Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05). These data clearly demonstrate that caffeine could mitigate lung inflammation induced by ischemia-reperfusion of the lower limbs.

No MeSH data available.


Related in: MedlinePlus

Total cells number (a) and protein concentration (b) in bronchoalveolar lavage fluid (BALF). Sham: the sham-operation group. Sham + Caf: the sham plus caffeine group. IR: the lower limb ischemia-reperfusion group. IR + Caf: the IR plus caffeine group. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control for the effects of the treatment vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at the comparable time point. One-way analysis of variance with the Bonferroni-Dunn test was used for multiple comparisons. The significance level was set at 0.05. Data were derived from 6 rats from each group and presented as mean ± standard deviation. ∗P < 0.05: the IR group versus the Sham group. #P < 0.05: the IR + Caf group versus the IR group.
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fig2: Total cells number (a) and protein concentration (b) in bronchoalveolar lavage fluid (BALF). Sham: the sham-operation group. Sham + Caf: the sham plus caffeine group. IR: the lower limb ischemia-reperfusion group. IR + Caf: the IR plus caffeine group. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control for the effects of the treatment vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at the comparable time point. One-way analysis of variance with the Bonferroni-Dunn test was used for multiple comparisons. The significance level was set at 0.05. Data were derived from 6 rats from each group and presented as mean ± standard deviation. ∗P < 0.05: the IR group versus the Sham group. #P < 0.05: the IR + Caf group versus the IR group.

Mentions: In BALF from the Sham group, the total cells number was 0.28 ± 0.13 × 106 cells/mL and the protein concentration was 0.03 ± 0.03 μg/mL (Figures 2(a) and 2(b)). The total cells number (0.39 ± 0.09 × 106 cells/mL) and protein concentration (0.14 ± 0.05 μg/mL) in BALF of the Sham + Caf groups were similar to those of the Sham group (Figures 2(a) and 2(b)). The total cells number (1.31 ± 0.26 × 106 cells/mL) and protein concentration (0.63 ± 0.22 μg/mL) of the IR group were significantly higher than those of the Sham group (P < 0.001 and =0.002, resp.; Figures 2(a) and 2(b)). Moreover, the total cells number (0.55 ± 0.14 × 106 cells/mL) and protein concentration (0.17 ± 0.03 μg/mL) of the IR + Caf group were significantly lower than those of the IR group (P < 0.001 and P = 0.008, resp.; Figures 2(a) and 2(b)).


Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats.

Chou WC, Kao MC, Yue CT, Tsai PS, Huang CJ - Mediators Inflamm. (2015)

Total cells number (a) and protein concentration (b) in bronchoalveolar lavage fluid (BALF). Sham: the sham-operation group. Sham + Caf: the sham plus caffeine group. IR: the lower limb ischemia-reperfusion group. IR + Caf: the IR plus caffeine group. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control for the effects of the treatment vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at the comparable time point. One-way analysis of variance with the Bonferroni-Dunn test was used for multiple comparisons. The significance level was set at 0.05. Data were derived from 6 rats from each group and presented as mean ± standard deviation. ∗P < 0.05: the IR group versus the Sham group. #P < 0.05: the IR + Caf group versus the IR group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig2: Total cells number (a) and protein concentration (b) in bronchoalveolar lavage fluid (BALF). Sham: the sham-operation group. Sham + Caf: the sham plus caffeine group. IR: the lower limb ischemia-reperfusion group. IR + Caf: the IR plus caffeine group. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control for the effects of the treatment vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at the comparable time point. One-way analysis of variance with the Bonferroni-Dunn test was used for multiple comparisons. The significance level was set at 0.05. Data were derived from 6 rats from each group and presented as mean ± standard deviation. ∗P < 0.05: the IR group versus the Sham group. #P < 0.05: the IR + Caf group versus the IR group.
Mentions: In BALF from the Sham group, the total cells number was 0.28 ± 0.13 × 106 cells/mL and the protein concentration was 0.03 ± 0.03 μg/mL (Figures 2(a) and 2(b)). The total cells number (0.39 ± 0.09 × 106 cells/mL) and protein concentration (0.14 ± 0.05 μg/mL) in BALF of the Sham + Caf groups were similar to those of the Sham group (Figures 2(a) and 2(b)). The total cells number (1.31 ± 0.26 × 106 cells/mL) and protein concentration (0.63 ± 0.22 μg/mL) of the IR group were significantly higher than those of the Sham group (P < 0.001 and =0.002, resp.; Figures 2(a) and 2(b)). Moreover, the total cells number (0.55 ± 0.14 × 106 cells/mL) and protein concentration (0.17 ± 0.03 μg/mL) of the IR + Caf group were significantly lower than those of the IR group (P < 0.001 and P = 0.008, resp.; Figures 2(a) and 2(b)).

Bottom Line: Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group.Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.).Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 289 Jianguo Road, Xindian District, New Taipei City 23142, Taiwan ; School of Medicine, Tzu Chi University, No. 701, Sec. 3, Zhongyang Road, Hualien 97004, Taiwan.

ABSTRACT
Reperfusion of ischemic limbs can induce inflammation and subsequently cause acute lung injury. Caffeine, a widely used psychostimulant, possesses potent anti-inflammatory capacity. We elucidated whether caffeine can mitigate lung inflammation caused by ischemia-reperfusion (IR) of the lower limbs. Adult male Sprague-Dawley rats were randomly allocated to receive IR, IR plus caffeine (IR + Caf group), sham-operation (Sham), or sham plus caffeine (n = 12 in each group). To induce IR, lower limbs were bilaterally tied by rubber bands high around each thigh for 3 hours followed by reperfusion for 3 hours. Caffeine (50 mg/kg, intraperitoneal injection) was administered immediately after reperfusion. Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group. Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.). Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05). These data clearly demonstrate that caffeine could mitigate lung inflammation induced by ischemia-reperfusion of the lower limbs.

No MeSH data available.


Related in: MedlinePlus