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Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats.

Chou WC, Kao MC, Yue CT, Tsai PS, Huang CJ - Mediators Inflamm. (2015)

Bottom Line: Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group.Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.).Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 289 Jianguo Road, Xindian District, New Taipei City 23142, Taiwan ; School of Medicine, Tzu Chi University, No. 701, Sec. 3, Zhongyang Road, Hualien 97004, Taiwan.

ABSTRACT
Reperfusion of ischemic limbs can induce inflammation and subsequently cause acute lung injury. Caffeine, a widely used psychostimulant, possesses potent anti-inflammatory capacity. We elucidated whether caffeine can mitigate lung inflammation caused by ischemia-reperfusion (IR) of the lower limbs. Adult male Sprague-Dawley rats were randomly allocated to receive IR, IR plus caffeine (IR + Caf group), sham-operation (Sham), or sham plus caffeine (n = 12 in each group). To induce IR, lower limbs were bilaterally tied by rubber bands high around each thigh for 3 hours followed by reperfusion for 3 hours. Caffeine (50 mg/kg, intraperitoneal injection) was administered immediately after reperfusion. Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group. Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.). Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05). These data clearly demonstrate that caffeine could mitigate lung inflammation induced by ischemia-reperfusion of the lower limbs.

No MeSH data available.


Related in: MedlinePlus

Representative microscopic findings of the lung tissues stained with hemotoxylin & eosin (100x). (a) The sham-operation (Sham) group revealed normal to mild lung inflammation characteristics. (b) The sham plus caffeine (Sham + Caf) group revealed normal to mild lung inflammation characteristics. (c) The lower limb ischemia-reperfusion (IR) group revealed severe lung inflammation characteristics. (d) The IR plus caffeine (IR + Caf) group revealed mild to moderate lung inflammation characteristics. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control the effects of vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at comparable time point.
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fig1: Representative microscopic findings of the lung tissues stained with hemotoxylin & eosin (100x). (a) The sham-operation (Sham) group revealed normal to mild lung inflammation characteristics. (b) The sham plus caffeine (Sham + Caf) group revealed normal to mild lung inflammation characteristics. (c) The lower limb ischemia-reperfusion (IR) group revealed severe lung inflammation characteristics. (d) The IR plus caffeine (IR + Caf) group revealed mild to moderate lung inflammation characteristics. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control the effects of vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at comparable time point.

Mentions: Histological analysis revealed normal to mild lung inflammation characteristics in the Sham and the Sham + Caf groups (Figures 1(a) and 1(b)). The lung tissues of the IR group revealed severe inflammation characteristics (Figure 1(c)). Moreover, the lung tissues of the IR + Caf group revealed mild to moderate lung inflammation characteristics (Figure 1(d)).


Caffeine Mitigates Lung Inflammation Induced by Ischemia-Reperfusion of Lower Limbs in Rats.

Chou WC, Kao MC, Yue CT, Tsai PS, Huang CJ - Mediators Inflamm. (2015)

Representative microscopic findings of the lung tissues stained with hemotoxylin & eosin (100x). (a) The sham-operation (Sham) group revealed normal to mild lung inflammation characteristics. (b) The sham plus caffeine (Sham + Caf) group revealed normal to mild lung inflammation characteristics. (c) The lower limb ischemia-reperfusion (IR) group revealed severe lung inflammation characteristics. (d) The IR plus caffeine (IR + Caf) group revealed mild to moderate lung inflammation characteristics. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control the effects of vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at comparable time point.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663348&req=5

fig1: Representative microscopic findings of the lung tissues stained with hemotoxylin & eosin (100x). (a) The sham-operation (Sham) group revealed normal to mild lung inflammation characteristics. (b) The sham plus caffeine (Sham + Caf) group revealed normal to mild lung inflammation characteristics. (c) The lower limb ischemia-reperfusion (IR) group revealed severe lung inflammation characteristics. (d) The IR plus caffeine (IR + Caf) group revealed mild to moderate lung inflammation characteristics. Rats of the Sham + Caf and the IR + Caf groups received caffeine (50 mg/kg, intraperitoneal injection) immediately after reperfusion. To control the effects of vehicle, rats of the Sham and the IR group received normal saline (1.0 mL, intraperitoneal injection) at comparable time point.
Mentions: Histological analysis revealed normal to mild lung inflammation characteristics in the Sham and the Sham + Caf groups (Figures 1(a) and 1(b)). The lung tissues of the IR group revealed severe inflammation characteristics (Figure 1(c)). Moreover, the lung tissues of the IR + Caf group revealed mild to moderate lung inflammation characteristics (Figure 1(d)).

Bottom Line: Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group.Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.).Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 289 Jianguo Road, Xindian District, New Taipei City 23142, Taiwan ; School of Medicine, Tzu Chi University, No. 701, Sec. 3, Zhongyang Road, Hualien 97004, Taiwan.

ABSTRACT
Reperfusion of ischemic limbs can induce inflammation and subsequently cause acute lung injury. Caffeine, a widely used psychostimulant, possesses potent anti-inflammatory capacity. We elucidated whether caffeine can mitigate lung inflammation caused by ischemia-reperfusion (IR) of the lower limbs. Adult male Sprague-Dawley rats were randomly allocated to receive IR, IR plus caffeine (IR + Caf group), sham-operation (Sham), or sham plus caffeine (n = 12 in each group). To induce IR, lower limbs were bilaterally tied by rubber bands high around each thigh for 3 hours followed by reperfusion for 3 hours. Caffeine (50 mg/kg, intraperitoneal injection) was administered immediately after reperfusion. Our histological assay data revealed characteristics of severe lung inflammation in the IR group and mild to moderate characteristic of lung inflammation in the IR + Caf group. Total cells number and protein concentration in bronchoalveolar lavage fluid of the IR group were significantly higher than those of the IR + Caf group (P < 0.001 and P = 0.008, resp.). Similarly, pulmonary concentrations of inflammatory mediators (tumor necrosis factor-α, interleukin-1β, and macrophage inflammatory protein-2) and pulmonary myeloperoxidase activity of the IR group were significantly higher than those of the IR + Caf group (all P < 0.05). These data clearly demonstrate that caffeine could mitigate lung inflammation induced by ischemia-reperfusion of the lower limbs.

No MeSH data available.


Related in: MedlinePlus