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Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells.

Zhang L, Dang RJ, Yang YM, Cui DC, Li P, Ni YL, Hao T, Wang C, Jiang XX, Fang NZ - Stem Cells Int (2015)

Bottom Line: With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production.The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4.DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, China ; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China ; Department of Biology and Chemical Engineering, Tongren University, Tongren, Guizhou 554300, China.

ABSTRACT
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

No MeSH data available.


Related in: MedlinePlus

NO production in OP9 and OP9DL1 cells in the presence of different stimulators. OP9 and OP9DL1 were stimulated with TNFα/IFNγ (each 2 ng/mL), LPS (10 μg/mL)/IL4 (25 ng/mL), or PMA (50 ng/mL)/ion (1 μg/mL) for 24 h, respectively. Supernatants were collected at 6, 12, and 24 h and used for NO assay, respectively.
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fig6: NO production in OP9 and OP9DL1 cells in the presence of different stimulators. OP9 and OP9DL1 were stimulated with TNFα/IFNγ (each 2 ng/mL), LPS (10 μg/mL)/IL4 (25 ng/mL), or PMA (50 ng/mL)/ion (1 μg/mL) for 24 h, respectively. Supernatants were collected at 6, 12, and 24 h and used for NO assay, respectively.

Mentions: NO has been shown to be involved in the immunomodulation of MSCs to multiple immune cells [11]. To examine whether NO is related to the effect of OP9 and OP9DL1 on mature T or B cell proliferation, we assayed the NO level in culture supernatants from OP9 and OP9DL1 stimulated with TNFα/IFNγ, LPS/IL4, or PMA/ion for 6, 12, and 24 h, respectively (Figure 6). Our data showed that NO production was indeed increased in the culture media from each group of stimulation factors, regardless of stimulation duration of OP9 or OP9DL1, as compared with that of the no stimulation group. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4 (Figure 6).


Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells.

Zhang L, Dang RJ, Yang YM, Cui DC, Li P, Ni YL, Hao T, Wang C, Jiang XX, Fang NZ - Stem Cells Int (2015)

NO production in OP9 and OP9DL1 cells in the presence of different stimulators. OP9 and OP9DL1 were stimulated with TNFα/IFNγ (each 2 ng/mL), LPS (10 μg/mL)/IL4 (25 ng/mL), or PMA (50 ng/mL)/ion (1 μg/mL) for 24 h, respectively. Supernatants were collected at 6, 12, and 24 h and used for NO assay, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4663344&req=5

fig6: NO production in OP9 and OP9DL1 cells in the presence of different stimulators. OP9 and OP9DL1 were stimulated with TNFα/IFNγ (each 2 ng/mL), LPS (10 μg/mL)/IL4 (25 ng/mL), or PMA (50 ng/mL)/ion (1 μg/mL) for 24 h, respectively. Supernatants were collected at 6, 12, and 24 h and used for NO assay, respectively.
Mentions: NO has been shown to be involved in the immunomodulation of MSCs to multiple immune cells [11]. To examine whether NO is related to the effect of OP9 and OP9DL1 on mature T or B cell proliferation, we assayed the NO level in culture supernatants from OP9 and OP9DL1 stimulated with TNFα/IFNγ, LPS/IL4, or PMA/ion for 6, 12, and 24 h, respectively (Figure 6). Our data showed that NO production was indeed increased in the culture media from each group of stimulation factors, regardless of stimulation duration of OP9 or OP9DL1, as compared with that of the no stimulation group. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4 (Figure 6).

Bottom Line: With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production.The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4.DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, China ; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China ; Department of Biology and Chemical Engineering, Tongren University, Tongren, Guizhou 554300, China.

ABSTRACT
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

No MeSH data available.


Related in: MedlinePlus