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Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells.

Zhang L, Dang RJ, Yang YM, Cui DC, Li P, Ni YL, Hao T, Wang C, Jiang XX, Fang NZ - Stem Cells Int (2015)

Bottom Line: With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production.The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4.DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, China ; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China ; Department of Biology and Chemical Engineering, Tongren University, Tongren, Guizhou 554300, China.

ABSTRACT
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

No MeSH data available.


Related in: MedlinePlus

Differentiation ability of OP9 or OP9DL1 to adipocytes and osteoblasts in vitro. (a) Differentiation into adipocytes was shown by Oil Red O staining at the indicated time points, respectively. (b) The osteogenesis of OP9 and OP9DL1 was assayed by ALP staining at different times, respectively.
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fig2: Differentiation ability of OP9 or OP9DL1 to adipocytes and osteoblasts in vitro. (a) Differentiation into adipocytes was shown by Oil Red O staining at the indicated time points, respectively. (b) The osteogenesis of OP9 and OP9DL1 was assayed by ALP staining at different times, respectively.

Mentions: Recently, there has been some debate on the effects of Notch receptor/ligand interaction on the differentiation of MSC into the osteocyte and adipocyte lineages [23, 24]. To investigate the effect of DL1 on OP9 differentiation, we examined the adipogenesis and osteogenesis of OP9 and OP9DL1 at different time intervals as indicated in Figures 2(a) and 2(b). Oil Red O staining revealed that the rate of adipocyte differentiation of OP9 cells was faster than that of OP9DL1 at each of the indicated time points (Figure 2(a)). Conversely, osteogenesis of OP9DL1 was more robust compared with that of OP9 cells, as determined by ALP staining (Figure 2(b)).


Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells.

Zhang L, Dang RJ, Yang YM, Cui DC, Li P, Ni YL, Hao T, Wang C, Jiang XX, Fang NZ - Stem Cells Int (2015)

Differentiation ability of OP9 or OP9DL1 to adipocytes and osteoblasts in vitro. (a) Differentiation into adipocytes was shown by Oil Red O staining at the indicated time points, respectively. (b) The osteogenesis of OP9 and OP9DL1 was assayed by ALP staining at different times, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663344&req=5

fig2: Differentiation ability of OP9 or OP9DL1 to adipocytes and osteoblasts in vitro. (a) Differentiation into adipocytes was shown by Oil Red O staining at the indicated time points, respectively. (b) The osteogenesis of OP9 and OP9DL1 was assayed by ALP staining at different times, respectively.
Mentions: Recently, there has been some debate on the effects of Notch receptor/ligand interaction on the differentiation of MSC into the osteocyte and adipocyte lineages [23, 24]. To investigate the effect of DL1 on OP9 differentiation, we examined the adipogenesis and osteogenesis of OP9 and OP9DL1 at different time intervals as indicated in Figures 2(a) and 2(b). Oil Red O staining revealed that the rate of adipocyte differentiation of OP9 cells was faster than that of OP9DL1 at each of the indicated time points (Figure 2(a)). Conversely, osteogenesis of OP9DL1 was more robust compared with that of OP9 cells, as determined by ALP staining (Figure 2(b)).

Bottom Line: With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production.The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4.DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, China ; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China ; Department of Biology and Chemical Engineering, Tongren University, Tongren, Guizhou 554300, China.

ABSTRACT
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

No MeSH data available.


Related in: MedlinePlus