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Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells.

Zhang L, Dang RJ, Yang YM, Cui DC, Li P, Ni YL, Hao T, Wang C, Jiang XX, Fang NZ - Stem Cells Int (2015)

Bottom Line: With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production.The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4.DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, China ; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China ; Department of Biology and Chemical Engineering, Tongren University, Tongren, Guizhou 554300, China.

ABSTRACT
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

No MeSH data available.


Related in: MedlinePlus

Comparisons between OP9 and OP9DL1 cells with regard to their immunophenotype and proliferation. (a) The indicated surface markers of OP9 or OP9DL1 cells were shown by FACS analysis, respectively. ((b)-(c)) The growth rate of OP9 and OP9DL1 cells was assayed by Ki67 or BrdU incorporation assays, respectively.
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fig1: Comparisons between OP9 and OP9DL1 cells with regard to their immunophenotype and proliferation. (a) The indicated surface markers of OP9 or OP9DL1 cells were shown by FACS analysis, respectively. ((b)-(c)) The growth rate of OP9 and OP9DL1 cells was assayed by Ki67 or BrdU incorporation assays, respectively.

Mentions: It has been demonstrated that the OP9 cell line is genuine MSCs [10]. To examine whether OP9 cells overexpressing DL1 show a different immunophenotype than conventional OP9 cells, we analyzed surface markers indicated in Figure 1(a). The flow cytometric data showed that DL1 expression in OP9DL1 cells is significantly higher than that in OP9 cells. MSC surface molecules including CD29, CD44, and Sca-l were positive in both OP9 and OP9DL1 cells, while MSCs surface molecules CD105, hematopoietic lineage markers CD34 and CD45, and endothelial cell marker CD31 were almost absent in both OP9 and OP9DL1 cell lines. Next, we examined the proliferation ability of OP9 and OP9DL1 by Ki67 and BrdU labeling assays, respectively. The growth rate between OP9 and OP9DL1 did not differ significantly (Figures 1(b) and 1(c)).


Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells.

Zhang L, Dang RJ, Yang YM, Cui DC, Li P, Ni YL, Hao T, Wang C, Jiang XX, Fang NZ - Stem Cells Int (2015)

Comparisons between OP9 and OP9DL1 cells with regard to their immunophenotype and proliferation. (a) The indicated surface markers of OP9 or OP9DL1 cells were shown by FACS analysis, respectively. ((b)-(c)) The growth rate of OP9 and OP9DL1 cells was assayed by Ki67 or BrdU incorporation assays, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663344&req=5

fig1: Comparisons between OP9 and OP9DL1 cells with regard to their immunophenotype and proliferation. (a) The indicated surface markers of OP9 or OP9DL1 cells were shown by FACS analysis, respectively. ((b)-(c)) The growth rate of OP9 and OP9DL1 cells was assayed by Ki67 or BrdU incorporation assays, respectively.
Mentions: It has been demonstrated that the OP9 cell line is genuine MSCs [10]. To examine whether OP9 cells overexpressing DL1 show a different immunophenotype than conventional OP9 cells, we analyzed surface markers indicated in Figure 1(a). The flow cytometric data showed that DL1 expression in OP9DL1 cells is significantly higher than that in OP9 cells. MSC surface molecules including CD29, CD44, and Sca-l were positive in both OP9 and OP9DL1 cells, while MSCs surface molecules CD105, hematopoietic lineage markers CD34 and CD45, and endothelial cell marker CD31 were almost absent in both OP9 and OP9DL1 cell lines. Next, we examined the proliferation ability of OP9 and OP9DL1 by Ki67 and BrdU labeling assays, respectively. The growth rate between OP9 and OP9DL1 did not differ significantly (Figures 1(b) and 1(c)).

Bottom Line: With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production.The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4.DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, China ; Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China ; Department of Biology and Chemical Engineering, Tongren University, Tongren, Guizhou 554300, China.

ABSTRACT
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.

No MeSH data available.


Related in: MedlinePlus