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Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population.

Kabzinski J, Mucha B, Cuchra M, Markiewicz L, Przybylowska K, Dziki A, Dziki L, Majsterek I - Oxid Med Cell Longev (2015)

Bottom Line: SNPs were identified within genes responsible for steps following glycosylase action in BER, and patients and healthy subjects were genotyped.Decreased BER activity was observed in lymphocyte extract from CRC patients and in cancer tissue extract, compared to healthy subjects.In addition, polymorphisms of EXO1, LIG3, and PolB may modulate the risk of colorectal cancer by decreasing (PolB) or increasing (LIG3 and EXO1) the chance of malignant transformation.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.

ABSTRACT
DNA oxidative lesions are widely considered as a potential risk factor for colorectal cancer development. The aim of this work was to determine the role of the efficiency of base excision repair, both in lymphocytes and in epithelial tissue, in patients with CRC and healthy subjects. SNPs were identified within genes responsible for steps following glycosylase action in BER, and patients and healthy subjects were genotyped. A radioisotopic BER assay was used for assessing repair efficiency and TaqMan for genotyping. Decreased BER activity was observed in lymphocyte extract from CRC patients and in cancer tissue extract, compared to healthy subjects. In addition, polymorphisms of EXO1, LIG3, and PolB may modulate the risk of colorectal cancer by decreasing (PolB) or increasing (LIG3 and EXO1) the chance of malignant transformation.

No MeSH data available.


Related in: MedlinePlus

A comparison of BER activity in the lymphocytes and tissue of CRC patients and healthy controls. Each electropherogram shows two fractions of DNA: 450 pb repaired and 185 pb unrepaired. Lanes 1-2 indicate lymphocyte BER efficiency while lanes 3-4 refer to BER in tissue. Samples are presented in the following order: K: positive control; DNA substrate did not contain uracil and so reflects 100% of repair; 1: healthy control; 2: colorectal cancer; 3: unchanged colon tissue; 4: colorectal cancer.
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fig2: A comparison of BER activity in the lymphocytes and tissue of CRC patients and healthy controls. Each electropherogram shows two fractions of DNA: 450 pb repaired and 185 pb unrepaired. Lanes 1-2 indicate lymphocyte BER efficiency while lanes 3-4 refer to BER in tissue. Samples are presented in the following order: K: positive control; DNA substrate did not contain uracil and so reflects 100% of repair; 1: healthy control; 2: colorectal cancer; 3: unchanged colon tissue; 4: colorectal cancer.

Mentions: In general, optical density detection of particular DNA bands revealed higher BER repair efficiency among cancer-free individuals than CRC patients in both lymphocytes and colon tissue samples. The percentage ratio of repaired to damaged fractions was found to be 89.67%/10.32% in lymphocytes taken from healthy subjects and 70.5%/29.5% in those of CRC patients. Examination of the ability of tissue protein extract to perform BER indicated a significantly greater repair level in normal tissue (68.11%/31.89%) than CRC tissue (58.36%/41.64%). The results of the BER assay analysis are presented in Figure 2.


Efficiency of Base Excision Repair of Oxidative DNA Damage and Its Impact on the Risk of Colorectal Cancer in the Polish Population.

Kabzinski J, Mucha B, Cuchra M, Markiewicz L, Przybylowska K, Dziki A, Dziki L, Majsterek I - Oxid Med Cell Longev (2015)

A comparison of BER activity in the lymphocytes and tissue of CRC patients and healthy controls. Each electropherogram shows two fractions of DNA: 450 pb repaired and 185 pb unrepaired. Lanes 1-2 indicate lymphocyte BER efficiency while lanes 3-4 refer to BER in tissue. Samples are presented in the following order: K: positive control; DNA substrate did not contain uracil and so reflects 100% of repair; 1: healthy control; 2: colorectal cancer; 3: unchanged colon tissue; 4: colorectal cancer.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663340&req=5

fig2: A comparison of BER activity in the lymphocytes and tissue of CRC patients and healthy controls. Each electropherogram shows two fractions of DNA: 450 pb repaired and 185 pb unrepaired. Lanes 1-2 indicate lymphocyte BER efficiency while lanes 3-4 refer to BER in tissue. Samples are presented in the following order: K: positive control; DNA substrate did not contain uracil and so reflects 100% of repair; 1: healthy control; 2: colorectal cancer; 3: unchanged colon tissue; 4: colorectal cancer.
Mentions: In general, optical density detection of particular DNA bands revealed higher BER repair efficiency among cancer-free individuals than CRC patients in both lymphocytes and colon tissue samples. The percentage ratio of repaired to damaged fractions was found to be 89.67%/10.32% in lymphocytes taken from healthy subjects and 70.5%/29.5% in those of CRC patients. Examination of the ability of tissue protein extract to perform BER indicated a significantly greater repair level in normal tissue (68.11%/31.89%) than CRC tissue (58.36%/41.64%). The results of the BER assay analysis are presented in Figure 2.

Bottom Line: SNPs were identified within genes responsible for steps following glycosylase action in BER, and patients and healthy subjects were genotyped.Decreased BER activity was observed in lymphocyte extract from CRC patients and in cancer tissue extract, compared to healthy subjects.In addition, polymorphisms of EXO1, LIG3, and PolB may modulate the risk of colorectal cancer by decreasing (PolB) or increasing (LIG3 and EXO1) the chance of malignant transformation.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Plac Hallera 1, 90-647 Lodz, Poland.

ABSTRACT
DNA oxidative lesions are widely considered as a potential risk factor for colorectal cancer development. The aim of this work was to determine the role of the efficiency of base excision repair, both in lymphocytes and in epithelial tissue, in patients with CRC and healthy subjects. SNPs were identified within genes responsible for steps following glycosylase action in BER, and patients and healthy subjects were genotyped. A radioisotopic BER assay was used for assessing repair efficiency and TaqMan for genotyping. Decreased BER activity was observed in lymphocyte extract from CRC patients and in cancer tissue extract, compared to healthy subjects. In addition, polymorphisms of EXO1, LIG3, and PolB may modulate the risk of colorectal cancer by decreasing (PolB) or increasing (LIG3 and EXO1) the chance of malignant transformation.

No MeSH data available.


Related in: MedlinePlus