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Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus

Effects of LTC on the lipid content and lipid peroxidation of erythrocyte membrane. (a) FC of erythrocyte membranes among HG, LTC groups (100, 200, and 300 mg/kg), and EG. (b) Effects of LTC on lipid peroxidation in erythrocyte membranes. ∗P < 0.05 versus HG; ∗∗∗P < 0.001 versus HG. FC: free cholesterol, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, LTC300 group: high dose LTC-treated group, MDA: Malondialdehyde.
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fig8: Effects of LTC on the lipid content and lipid peroxidation of erythrocyte membrane. (a) FC of erythrocyte membranes among HG, LTC groups (100, 200, and 300 mg/kg), and EG. (b) Effects of LTC on lipid peroxidation in erythrocyte membranes. ∗P < 0.05 versus HG; ∗∗∗P < 0.001 versus HG. FC: free cholesterol, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, LTC300 group: high dose LTC-treated group, MDA: Malondialdehyde.

Mentions: Many factors are probably associated with the improved osmotic fragility and deformability in erythrocytes, such as the erythrocyte skeleton, membrane lipid composition, and lipid peroxidation. In this study, the erythrocyte skeleton was studied by SDS-PAGE of erythrocyte ghosts. No significant changes of the major proteins, such as α-spectrin (band 1), β-spectrin (band 2), band 3, and 4.1 in membrane protein fractions, were observed among LTC- and ezetimibe-treated and untreated mice (Figure 7). As for lipid composition of the erythrocyte membrane, only ezetimibe had significantly decreased the FC level of erythrocyte ghost (P < 0.01, Figure 8(a)).


Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Effects of LTC on the lipid content and lipid peroxidation of erythrocyte membrane. (a) FC of erythrocyte membranes among HG, LTC groups (100, 200, and 300 mg/kg), and EG. (b) Effects of LTC on lipid peroxidation in erythrocyte membranes. ∗P < 0.05 versus HG; ∗∗∗P < 0.001 versus HG. FC: free cholesterol, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, LTC300 group: high dose LTC-treated group, MDA: Malondialdehyde.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663336&req=5

fig8: Effects of LTC on the lipid content and lipid peroxidation of erythrocyte membrane. (a) FC of erythrocyte membranes among HG, LTC groups (100, 200, and 300 mg/kg), and EG. (b) Effects of LTC on lipid peroxidation in erythrocyte membranes. ∗P < 0.05 versus HG; ∗∗∗P < 0.001 versus HG. FC: free cholesterol, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, LTC300 group: high dose LTC-treated group, MDA: Malondialdehyde.
Mentions: Many factors are probably associated with the improved osmotic fragility and deformability in erythrocytes, such as the erythrocyte skeleton, membrane lipid composition, and lipid peroxidation. In this study, the erythrocyte skeleton was studied by SDS-PAGE of erythrocyte ghosts. No significant changes of the major proteins, such as α-spectrin (band 1), β-spectrin (band 2), band 3, and 4.1 in membrane protein fractions, were observed among LTC- and ezetimibe-treated and untreated mice (Figure 7). As for lipid composition of the erythrocyte membrane, only ezetimibe had significantly decreased the FC level of erythrocyte ghost (P < 0.01, Figure 8(a)).

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus