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Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus

Osmotic fragility of erythrocytes in ApoE−/− mice of HG, LTC groups (100, 200, and 300 mg/kg), and EG: the percentages of hemolysis (%) rate against osmotic pressures (mOsm/kg). The results reflect the outcome of experiments conducted on 9 ~ 10 mice for each measurement. ∗∗∗P < 0.001 versus HG; #P < 0.05 versus EG. HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.
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fig5: Osmotic fragility of erythrocytes in ApoE−/− mice of HG, LTC groups (100, 200, and 300 mg/kg), and EG: the percentages of hemolysis (%) rate against osmotic pressures (mOsm/kg). The results reflect the outcome of experiments conducted on 9 ~ 10 mice for each measurement. ∗∗∗P < 0.001 versus HG; #P < 0.05 versus EG. HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.

Mentions: The osmotic fragility of erythrocytes reflects their membrane ability to maintain the structural integrity. As shown in Figure 5(a), all the erythrocytes remained intact at 0.01 mol/L PBS solution (295 mOsm/kg) in each group. The percentage of fragmentation rose gradually with the decrease of PBS concentration. From the osmotic pressure 130 to 175 mOsm/kg, integrated erythrocytes appeared more frequently in all the doses of LTC- and ezetimibe-treated mice than in those of untreated ones, which caused the left-shift of osmotic fragility curve, indicating that the erythrocytes of drug-treated mice were healthier compared to those of the HFD-induced mice. Besides, at the osmotic pressure of 145 mOsm/kg, the antihemolysis effects of TC200 and LTC300 treatments were better than that of ezetimibe treatment (P < 0.05) (Figure 5(b)), indicating that LTC was more effective in the improvement of osmotic fragility than ezetimibe.


Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Osmotic fragility of erythrocytes in ApoE−/− mice of HG, LTC groups (100, 200, and 300 mg/kg), and EG: the percentages of hemolysis (%) rate against osmotic pressures (mOsm/kg). The results reflect the outcome of experiments conducted on 9 ~ 10 mice for each measurement. ∗∗∗P < 0.001 versus HG; #P < 0.05 versus EG. HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663336&req=5

fig5: Osmotic fragility of erythrocytes in ApoE−/− mice of HG, LTC groups (100, 200, and 300 mg/kg), and EG: the percentages of hemolysis (%) rate against osmotic pressures (mOsm/kg). The results reflect the outcome of experiments conducted on 9 ~ 10 mice for each measurement. ∗∗∗P < 0.001 versus HG; #P < 0.05 versus EG. HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.
Mentions: The osmotic fragility of erythrocytes reflects their membrane ability to maintain the structural integrity. As shown in Figure 5(a), all the erythrocytes remained intact at 0.01 mol/L PBS solution (295 mOsm/kg) in each group. The percentage of fragmentation rose gradually with the decrease of PBS concentration. From the osmotic pressure 130 to 175 mOsm/kg, integrated erythrocytes appeared more frequently in all the doses of LTC- and ezetimibe-treated mice than in those of untreated ones, which caused the left-shift of osmotic fragility curve, indicating that the erythrocytes of drug-treated mice were healthier compared to those of the HFD-induced mice. Besides, at the osmotic pressure of 145 mOsm/kg, the antihemolysis effects of TC200 and LTC300 treatments were better than that of ezetimibe treatment (P < 0.05) (Figure 5(b)), indicating that LTC was more effective in the improvement of osmotic fragility than ezetimibe.

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus