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Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus

Deformability of erythrocytes in ApoE−/− mice. (a) The deformation curve integral area of erythrocytes. (b) The (DI)max of erythrocytes. The results reflect the outcome of experiments conducted on 9~10 mice for each measurement. ∗P < 0.05 versus HG; ∗∗P < 0.01 versus HG. DI: erythrocyte deformation index, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.
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fig4: Deformability of erythrocytes in ApoE−/− mice. (a) The deformation curve integral area of erythrocytes. (b) The (DI)max of erythrocytes. The results reflect the outcome of experiments conducted on 9~10 mice for each measurement. ∗P < 0.05 versus HG; ∗∗P < 0.01 versus HG. DI: erythrocyte deformation index, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.

Mentions: Because erythrocyte deformability is a major factor of WBV at higher shear rates [34], LTC could improve the erythrocyte deformability in mice suffering from hypercholesterolemia. The changes of erythrocyte deformability and osmotic fragility had been investigated in the following studies. The erythrocyte deformability and osmotic fragility reflect the combination of membrane flexibility [35]. Previous studies on human subjects suggested that the erythrocyte deformability index (DI)max significantly decreased in hypercholesterolemia [36]. Our study found that the deformation curve integral area of erythrocyte increased after treatment of ezetimibe and LTC (P < 0.05), and the significant difference occurred at doses of 200 and 300 mg/kg of LTC-treated groups (P < 0.05, Figure 4(a)). Meanwhile, the (DI)max of erythrocyte significantly raised after 300 mg/kg of LTC or ezetimibe treatment (P < 0.05, Figure 4(b)).


Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Deformability of erythrocytes in ApoE−/− mice. (a) The deformation curve integral area of erythrocytes. (b) The (DI)max of erythrocytes. The results reflect the outcome of experiments conducted on 9~10 mice for each measurement. ∗P < 0.05 versus HG; ∗∗P < 0.01 versus HG. DI: erythrocyte deformation index, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663336&req=5

fig4: Deformability of erythrocytes in ApoE−/− mice. (a) The deformation curve integral area of erythrocytes. (b) The (DI)max of erythrocytes. The results reflect the outcome of experiments conducted on 9~10 mice for each measurement. ∗P < 0.05 versus HG; ∗∗P < 0.01 versus HG. DI: erythrocyte deformation index, HFD: high fat diet, HG: HFD-induced group, LTC: Longxuetongluo Capsule, LTC100 group: low dose LTC-treated group, LTC200 group: middle dose LTC-treated group, and LTC300 group: high dose LTC-treated group.
Mentions: Because erythrocyte deformability is a major factor of WBV at higher shear rates [34], LTC could improve the erythrocyte deformability in mice suffering from hypercholesterolemia. The changes of erythrocyte deformability and osmotic fragility had been investigated in the following studies. The erythrocyte deformability and osmotic fragility reflect the combination of membrane flexibility [35]. Previous studies on human subjects suggested that the erythrocyte deformability index (DI)max significantly decreased in hypercholesterolemia [36]. Our study found that the deformation curve integral area of erythrocyte increased after treatment of ezetimibe and LTC (P < 0.05), and the significant difference occurred at doses of 200 and 300 mg/kg of LTC-treated groups (P < 0.05, Figure 4(a)). Meanwhile, the (DI)max of erythrocyte significantly raised after 300 mg/kg of LTC or ezetimibe treatment (P < 0.05, Figure 4(b)).

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus