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Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of the main components in LTC.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig2: Chemical structures of the main components in LTC.

Mentions: LTC was provided by Jiangsu Kanion Pharmaceutical Co. Ltd. (Jiangsu, China) [19–21], and the content of total phenols accounted for 70.07% as determined by colorimetry method [21]. The chemical profile of LTC is shown in Figure 1. HPLC analysis was performed according to the previous method with slight modification [20, 21]. Briefly, the sample was run on an Agilent XDB-C18 column (250 × 4.6 mm i.d., 5 μm, Agilent Technologies, Palo Alto, CA, USA) and the column temperature was set at 30°C. The mobile phase was composed of acetonitrile (A) and 0.1% aqueous formic acid (B) and delivered at a total flow rate of 1.0 mL/min following a gradient program: 0–35 min, 20%–31% A; 35–45 min, 31%–31% A; and 45–80 min, 31%–55% A; UV absorption over 190–400 nm was recorded by Diode Array Detector (DAD), and fixed wavelength of 280 nm was used for the detection of phenolic constituents. The injection volume was set at 10 μL. Ten phenolic compounds in LTC were identified as 7,4′-dihydroxyflavone (1, tR 16.91 min), loureirin D (2, tR 22.56 min), 7,4′-dihydroxyhomoisoflavanone (3, tR 28.39 min), loureirin C (4, tR 32.93 min), 3,4′-dihydroxy-5-methoxystilbene (5, tR 43.59 min), 5,7-dihydroxy-4′-methoxy-8-methylflavan (6, tR 56.61 min), 4-hydroxy-2,4′-dimethoxydihydrochalcone (7, tR 62.78 min), loureirin A (8, tR 64.70 min), loureirin B (9, tR 65.98 min), and pterostilbene (10, tR 72.48 min) by comparison of their retention times and UV spectra with those of authentic compounds [22, 23]. The structures of the identified compounds are shown in Figure 2. Furthermore, the content of two main phenolic compounds, 7,4′-dihydroxyflavone (0.69%) and loureirin B (0.83%), in the total phenol extract of LTC was determined by using our patented analytical method [21].


Longxuetongluo Capsule Improves Erythrocyte Function against Lipid Peroxidation and Abnormal Hemorheological Parameters in High Fat Diet-Induced ApoE-/- Mice.

Zheng J, Liu B, Lun Q, Yao W, Zhao Y, Xiao W, Huang W, Wang Y, Li J, Tu P - Oxid Med Cell Longev (2015)

Chemical structures of the main components in LTC.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663336&req=5

fig2: Chemical structures of the main components in LTC.
Mentions: LTC was provided by Jiangsu Kanion Pharmaceutical Co. Ltd. (Jiangsu, China) [19–21], and the content of total phenols accounted for 70.07% as determined by colorimetry method [21]. The chemical profile of LTC is shown in Figure 1. HPLC analysis was performed according to the previous method with slight modification [20, 21]. Briefly, the sample was run on an Agilent XDB-C18 column (250 × 4.6 mm i.d., 5 μm, Agilent Technologies, Palo Alto, CA, USA) and the column temperature was set at 30°C. The mobile phase was composed of acetonitrile (A) and 0.1% aqueous formic acid (B) and delivered at a total flow rate of 1.0 mL/min following a gradient program: 0–35 min, 20%–31% A; 35–45 min, 31%–31% A; and 45–80 min, 31%–55% A; UV absorption over 190–400 nm was recorded by Diode Array Detector (DAD), and fixed wavelength of 280 nm was used for the detection of phenolic constituents. The injection volume was set at 10 μL. Ten phenolic compounds in LTC were identified as 7,4′-dihydroxyflavone (1, tR 16.91 min), loureirin D (2, tR 22.56 min), 7,4′-dihydroxyhomoisoflavanone (3, tR 28.39 min), loureirin C (4, tR 32.93 min), 3,4′-dihydroxy-5-methoxystilbene (5, tR 43.59 min), 5,7-dihydroxy-4′-methoxy-8-methylflavan (6, tR 56.61 min), 4-hydroxy-2,4′-dimethoxydihydrochalcone (7, tR 62.78 min), loureirin A (8, tR 64.70 min), loureirin B (9, tR 65.98 min), and pterostilbene (10, tR 72.48 min) by comparison of their retention times and UV spectra with those of authentic compounds [22, 23]. The structures of the identified compounds are shown in Figure 2. Furthermore, the content of two main phenolic compounds, 7,4′-dihydroxyflavone (0.69%) and loureirin B (0.83%), in the total phenol extract of LTC was determined by using our patented analytical method [21].

Bottom Line: Phenolic compounds are considered to be responsible for its main biological activities.In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice.In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

View Article: PubMed Central - PubMed

Affiliation: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Chinese dragon's blood, the red resin of Dracaena cochinchinensis, one of the renowned traditional medicines, has been used to facilitate blood circulation and disperse blood stasis for thousands of years. Phenolic compounds are considered to be responsible for its main biological activities. In this study, total phenolic compounds of Chinese dragon's blood were made into capsule (Longxuetongluo Capsule, LTC) and their effects on the abnormal hemorheological properties were examined by high fat diet (HFD) induced ApoE-/- mice. Compared to the model group, LTC recovered the abnormal hemorheological parameters in HFD-induced ApoE-/- mice by reducing whole blood viscosity (WBV) at high rate and improving erythrocyte function. In conclusion, LTC could ameliorate erythrocyte deformability and osmotic fragility through the reduction of lipid peroxidation on plasma and erythrocyte membranes in HFD-induced ApoE-/- mice, which supported the traditional uses of Chinese dragon's blood as an effective agent for improving blood microcirculation in hypercholesterolemia.

No MeSH data available.


Related in: MedlinePlus