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Adipose-Derived Stem Cells Alleviate Radiation-Induced Muscular Fibrosis by Suppressing the Expression of TGF-β1.

Sun W, Ni X, Sun S, Cai L, Yu J, Wang J, Nie B, Sun Z, Ni X, Cao X - Stem Cells Int (2015)

Bottom Line: Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle.Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis.ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy, The Affiliated Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou 21300, China.

ABSTRACT
We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal muscle served as normal control. Skeletal muscle tissues were collected from eight rabbits in each group at 1 w, 4 w, 8 w, and 26 w after irradiation. Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle. The proportion of the area of collagen fibers to the total area in sections of ASCs group was lower than those of PBS groups at 4 w, 8 w, and 26 w after irradiation. Significant decrease was noted in the integrated optimal density of the transforming growth factor β1 (TGF-β1) in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation. Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis. ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

No MeSH data available.


Related in: MedlinePlus

IHC analysis of TGF-β1 in muscle tissue at different time points after irradiation (×200). No significant expression of TGF-β1 was noted in the PBS group (a) and ASCs group (b) at 1 w after irradiation. The expression of TGF-β1 increased gradually in both PBS group (c, e, and g) and ASCs group (d, f, and h) at 4 w, 8 w, and 26 w; however, the expression of TGF-β1 in ASCs group was lower than those of PBS group at the same time point after irradiation.
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fig6: IHC analysis of TGF-β1 in muscle tissue at different time points after irradiation (×200). No significant expression of TGF-β1 was noted in the PBS group (a) and ASCs group (b) at 1 w after irradiation. The expression of TGF-β1 increased gradually in both PBS group (c, e, and g) and ASCs group (d, f, and h) at 4 w, 8 w, and 26 w; however, the expression of TGF-β1 in ASCs group was lower than those of PBS group at the same time point after irradiation.

Mentions: TGF-β1 was mainly expressed in the cytoplasm of the muscle cells and was labeled in a brown color. In the normal group, the expression of TGF-β1 was low at all time points, and no significant difference was noted in the IOD at each time point. In addition, compared with the normal control group, remarkable increases were revealed in the IOD of TGF-β1 in ASCs group and PBS group at 1 w, 4 w, 8 w, and 26 w, respectively (P < 0.05). However, significant decrease was noted in the IOD of TGF-β1 in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation (t = 3.787, 16.454, and 15.171, P < 0.05, Figures 6 and 7). Moreover, Western blot analysis revealed that the expression of TGF-β1 was lower in the ASCs group in comparison with those of the PBS group at each time point (t = 5.939, 10.249, 5.986, and 7.986, P < 0.05, Figure 8).


Adipose-Derived Stem Cells Alleviate Radiation-Induced Muscular Fibrosis by Suppressing the Expression of TGF-β1.

Sun W, Ni X, Sun S, Cai L, Yu J, Wang J, Nie B, Sun Z, Ni X, Cao X - Stem Cells Int (2015)

IHC analysis of TGF-β1 in muscle tissue at different time points after irradiation (×200). No significant expression of TGF-β1 was noted in the PBS group (a) and ASCs group (b) at 1 w after irradiation. The expression of TGF-β1 increased gradually in both PBS group (c, e, and g) and ASCs group (d, f, and h) at 4 w, 8 w, and 26 w; however, the expression of TGF-β1 in ASCs group was lower than those of PBS group at the same time point after irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4663335&req=5

fig6: IHC analysis of TGF-β1 in muscle tissue at different time points after irradiation (×200). No significant expression of TGF-β1 was noted in the PBS group (a) and ASCs group (b) at 1 w after irradiation. The expression of TGF-β1 increased gradually in both PBS group (c, e, and g) and ASCs group (d, f, and h) at 4 w, 8 w, and 26 w; however, the expression of TGF-β1 in ASCs group was lower than those of PBS group at the same time point after irradiation.
Mentions: TGF-β1 was mainly expressed in the cytoplasm of the muscle cells and was labeled in a brown color. In the normal group, the expression of TGF-β1 was low at all time points, and no significant difference was noted in the IOD at each time point. In addition, compared with the normal control group, remarkable increases were revealed in the IOD of TGF-β1 in ASCs group and PBS group at 1 w, 4 w, 8 w, and 26 w, respectively (P < 0.05). However, significant decrease was noted in the IOD of TGF-β1 in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation (t = 3.787, 16.454, and 15.171, P < 0.05, Figures 6 and 7). Moreover, Western blot analysis revealed that the expression of TGF-β1 was lower in the ASCs group in comparison with those of the PBS group at each time point (t = 5.939, 10.249, 5.986, and 7.986, P < 0.05, Figure 8).

Bottom Line: Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle.Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis.ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy, The Affiliated Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou 21300, China.

ABSTRACT
We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal muscle served as normal control. Skeletal muscle tissues were collected from eight rabbits in each group at 1 w, 4 w, 8 w, and 26 w after irradiation. Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle. The proportion of the area of collagen fibers to the total area in sections of ASCs group was lower than those of PBS groups at 4 w, 8 w, and 26 w after irradiation. Significant decrease was noted in the integrated optimal density of the transforming growth factor β1 (TGF-β1) in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation. Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis. ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

No MeSH data available.


Related in: MedlinePlus