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Adipose-Derived Stem Cells Alleviate Radiation-Induced Muscular Fibrosis by Suppressing the Expression of TGF-β1.

Sun W, Ni X, Sun S, Cai L, Yu J, Wang J, Nie B, Sun Z, Ni X, Cao X - Stem Cells Int (2015)

Bottom Line: Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle.Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis.ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy, The Affiliated Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou 21300, China.

ABSTRACT
We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal muscle served as normal control. Skeletal muscle tissues were collected from eight rabbits in each group at 1 w, 4 w, 8 w, and 26 w after irradiation. Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle. The proportion of the area of collagen fibers to the total area in sections of ASCs group was lower than those of PBS groups at 4 w, 8 w, and 26 w after irradiation. Significant decrease was noted in the integrated optimal density of the transforming growth factor β1 (TGF-β1) in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation. Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis. ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

No MeSH data available.


Related in: MedlinePlus

(a) Morphology of ASCs (P2) under an inverted phase contrast microscope; (b) CM-Dil labeled ASCs (P2) under a fluorescence microscope. The images were observed under a magnification of 100x.
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fig1: (a) Morphology of ASCs (P2) under an inverted phase contrast microscope; (b) CM-Dil labeled ASCs (P2) under a fluorescence microscope. The images were observed under a magnification of 100x.

Mentions: The microscopy revealed that fibroblast-like ASCs exhibited long spindle-like shape with clear cellular boundaries. For each cell, the nucleus was well stacked in the center (Figure 1(a)). Cells labeled with CM-Dil showed red membrane and cytoplasm. Morphological evaluation of the cells showed no statistical difference after CM-Dil labeling (Figure 1(b)).


Adipose-Derived Stem Cells Alleviate Radiation-Induced Muscular Fibrosis by Suppressing the Expression of TGF-β1.

Sun W, Ni X, Sun S, Cai L, Yu J, Wang J, Nie B, Sun Z, Ni X, Cao X - Stem Cells Int (2015)

(a) Morphology of ASCs (P2) under an inverted phase contrast microscope; (b) CM-Dil labeled ASCs (P2) under a fluorescence microscope. The images were observed under a magnification of 100x.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663335&req=5

fig1: (a) Morphology of ASCs (P2) under an inverted phase contrast microscope; (b) CM-Dil labeled ASCs (P2) under a fluorescence microscope. The images were observed under a magnification of 100x.
Mentions: The microscopy revealed that fibroblast-like ASCs exhibited long spindle-like shape with clear cellular boundaries. For each cell, the nucleus was well stacked in the center (Figure 1(a)). Cells labeled with CM-Dil showed red membrane and cytoplasm. Morphological evaluation of the cells showed no statistical difference after CM-Dil labeling (Figure 1(b)).

Bottom Line: Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle.Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis.ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy, The Affiliated Changzhou No. 2 People's Hospital, Nanjing Medical University, Changzhou 21300, China.

ABSTRACT
We aim to investigate the effects of adipose-derived stem cells (ASCs) transplantation on irradiation-induced skeletal muscle fibrosis. Sixty-four rabbits were randomly divided into ASCs group and PBS group followed by irradiation at unilateral hip with a single dose of 80 Gy. Nonirradiated side with normal skeletal muscle served as normal control. Skeletal muscle tissues were collected from eight rabbits in each group at 1 w, 4 w, 8 w, and 26 w after irradiation. Migration of ASCs was observed in the peripheral tissues along the needle passage in the injured muscle. The proportion of the area of collagen fibers to the total area in sections of ASCs group was lower than those of PBS groups at 4 w, 8 w, and 26 w after irradiation. Significant decrease was noted in the integrated optimal density of the transforming growth factor β1 (TGF-β1) in the ASCs group compared with those of PBS group at 4 w, 8 w, and 26 w after irradiation. Moreover, the expression of TGF-β1 was lower in the ASCs group compared to those of the PBS group at each time point determined by Western blot analysis. ASCs transplantation could alleviate irradiation fibrosis by suppressing the level of TGF-β1 in the irradiated skeletal muscle.

No MeSH data available.


Related in: MedlinePlus