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Investigation of Fatty Acid Ketohydrazone Modified Liposome's Properties as a Drug Carrier.

Hayashi K, Kiriishi M, Suga K, Okamoto Y, Umakoshi H - J Drug Deliv (2015)

Bottom Line: The interface of the P-KH modified liposome at acidic pH was found to become more hydrophobic and less fluidic as compared with that at neutral pH; that is, P-KH modified liposome became more rigid structure.Therefore, it seems that the P-KH modified liposome could protect encapsulated drugs from the enzymes in the lysosome.This study shows the novel approach about design of pH-responsive liposomes based on the membrane properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Institute of Technology, Nara College, 22 Yata-cho, Yamatokoriyama, Nara 639-1080, Japan.

ABSTRACT
pH-responsive liposomes were prepared by modifying the liposome with acid-cleaving amphiphiles. Palmitic ketohydrazone (P-KH) or stearic ketohydrazone (S-KH), composed of hydrophilic sugar headgroup and hydrophobic acyl chain, was used as a modifier of the DMPC liposome. Because the ketohydrazone group of P-KH or S-KH was cleaved at low pH conditions (

No MeSH data available.


Related in: MedlinePlus

Drug delivery efficiency of P-KH liposome and S-KH liposome. (a) Quantitative evaluation of drug delivery efficiency by flow cytometer. (b) Images of confocal laser microscopy of colon 26 cells which were treated with fluorescence labeled P-KH liposome.
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fig3: Drug delivery efficiency of P-KH liposome and S-KH liposome. (a) Quantitative evaluation of drug delivery efficiency by flow cytometer. (b) Images of confocal laser microscopy of colon 26 cells which were treated with fluorescence labeled P-KH liposome.

Mentions: In order to evaluate the drug delivery efficiency, the uptake of three kinds of liposomes (such as DMPC liposome, P-KH liposome, and S-KH liposome) by colon 26 cells was evaluated by using flow cytometer (Figure 3(a)). This result shows that colon 26 cells took up similar amount of these liposomes. Therefore, both P-KH liposome and S-KH liposome can deliver the drug to the inside of cells and can also be applied as a drug carrier. Moreover, colon 26 cells were observed by confocal laser microscopy when colon 26 cells were treated with fluorescence labeled P-KH liposome (Figure 3(b)). Red fluorescence was observed at the inside of the cells. This result shows that colon 26 cells took up the P-KH liposome by endocytotic mechanism, judging from our previous report [20].


Investigation of Fatty Acid Ketohydrazone Modified Liposome's Properties as a Drug Carrier.

Hayashi K, Kiriishi M, Suga K, Okamoto Y, Umakoshi H - J Drug Deliv (2015)

Drug delivery efficiency of P-KH liposome and S-KH liposome. (a) Quantitative evaluation of drug delivery efficiency by flow cytometer. (b) Images of confocal laser microscopy of colon 26 cells which were treated with fluorescence labeled P-KH liposome.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663332&req=5

fig3: Drug delivery efficiency of P-KH liposome and S-KH liposome. (a) Quantitative evaluation of drug delivery efficiency by flow cytometer. (b) Images of confocal laser microscopy of colon 26 cells which were treated with fluorescence labeled P-KH liposome.
Mentions: In order to evaluate the drug delivery efficiency, the uptake of three kinds of liposomes (such as DMPC liposome, P-KH liposome, and S-KH liposome) by colon 26 cells was evaluated by using flow cytometer (Figure 3(a)). This result shows that colon 26 cells took up similar amount of these liposomes. Therefore, both P-KH liposome and S-KH liposome can deliver the drug to the inside of cells and can also be applied as a drug carrier. Moreover, colon 26 cells were observed by confocal laser microscopy when colon 26 cells were treated with fluorescence labeled P-KH liposome (Figure 3(b)). Red fluorescence was observed at the inside of the cells. This result shows that colon 26 cells took up the P-KH liposome by endocytotic mechanism, judging from our previous report [20].

Bottom Line: The interface of the P-KH modified liposome at acidic pH was found to become more hydrophobic and less fluidic as compared with that at neutral pH; that is, P-KH modified liposome became more rigid structure.Therefore, it seems that the P-KH modified liposome could protect encapsulated drugs from the enzymes in the lysosome.This study shows the novel approach about design of pH-responsive liposomes based on the membrane properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, National Institute of Technology, Nara College, 22 Yata-cho, Yamatokoriyama, Nara 639-1080, Japan.

ABSTRACT
pH-responsive liposomes were prepared by modifying the liposome with acid-cleaving amphiphiles. Palmitic ketohydrazone (P-KH) or stearic ketohydrazone (S-KH), composed of hydrophilic sugar headgroup and hydrophobic acyl chain, was used as a modifier of the DMPC liposome. Because the ketohydrazone group of P-KH or S-KH was cleaved at low pH conditions (

No MeSH data available.


Related in: MedlinePlus