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Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus

The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 2 g/kg of glucose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 compared with the control.
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Related In: Results  -  Collection


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fig4: The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 2 g/kg of glucose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 compared with the control.

Mentions: In normal rats, none of the groups treated with the 50% ethanol extract of O. stamineus showed any significant decreases in the blood glucose levels. Only acarbose produced a significant (P < 0.05) blood glucose lowering effect after glucose loading (Figure 4(a)). Similarly, using diabetic rats, none of the administered doses of the extract caused significant reduction in the blood glucose levels of glucose-loaded STZ-induced diabetic rats (Figure 4(b)). The acarbose-treated diabetic rats also did not show any significant reduction in the blood glucose levels.


Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 2 g/kg of glucose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 compared with the control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663316&req=5

fig4: The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 2 g/kg of glucose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 compared with the control.
Mentions: In normal rats, none of the groups treated with the 50% ethanol extract of O. stamineus showed any significant decreases in the blood glucose levels. Only acarbose produced a significant (P < 0.05) blood glucose lowering effect after glucose loading (Figure 4(a)). Similarly, using diabetic rats, none of the administered doses of the extract caused significant reduction in the blood glucose levels of glucose-loaded STZ-induced diabetic rats (Figure 4(b)). The acarbose-treated diabetic rats also did not show any significant reduction in the blood glucose levels.

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus