Limits...
Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus

The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 4 g/kg of sucrose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4663316&req=5

fig3: The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 4 g/kg of sucrose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.

Mentions: In normal rats, 250 mg/kg and 500 mg/kg of the 50% ethanol extract of O. stamineus did not show any reduction in blood glucose levels after sucrose loading. However, 1000 mg/kg of the extract managed to lower the blood glucose levels significantly (P < 0.05) compared to the control group (Figure 3(a)). Acarbose given at a dose of 10 mg/kg also produced a significant (P < 0.05) blood glucose lowering response in the sucrose-loaded normal rats. In diabetic rats, none of the extract-treated groups (250 mg/kg, 500 mg/kg, and 1000 mg/kg) showed any significant decrease in the blood glucose levels as compared to the diabetic control group (Figure 3(b)), whereas acarbose produced a significant (P < 0.05) blood glucose lowering effect in the sucrose-loaded STZ-induced diabetic rats.


Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 4 g/kg of sucrose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663316&req=5

fig3: The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 4 g/kg of sucrose. Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.
Mentions: In normal rats, 250 mg/kg and 500 mg/kg of the 50% ethanol extract of O. stamineus did not show any reduction in blood glucose levels after sucrose loading. However, 1000 mg/kg of the extract managed to lower the blood glucose levels significantly (P < 0.05) compared to the control group (Figure 3(a)). Acarbose given at a dose of 10 mg/kg also produced a significant (P < 0.05) blood glucose lowering response in the sucrose-loaded normal rats. In diabetic rats, none of the extract-treated groups (250 mg/kg, 500 mg/kg, and 1000 mg/kg) showed any significant decrease in the blood glucose levels as compared to the diabetic control group (Figure 3(b)), whereas acarbose produced a significant (P < 0.05) blood glucose lowering effect in the sucrose-loaded STZ-induced diabetic rats.

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus