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Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus

The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 3 g/kg of starch (a). Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.
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Related In: Results  -  Collection


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fig2: The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 3 g/kg of starch (a). Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.

Mentions: Using normal rats, extract-treated groups receiving 250 mg/kg and 500 mg/kg of the 50% ethanol extract of O. stamineus did not show any significant decrease in blood glucose levels (Figure 2(a)) compared to control rats. Only a dose of 1000 mg/kg of the extract reduced the blood glucose levels significantly (P < 0.05) after starch loading. Administered at a dose of 10 mg/kg, acarbose also significantly (P < 0.05) reduced the blood glucose levels of starch loaded normal rats. Likewise, using diabetic rats, only the group treated with 1000 mg/kg showed significant (P < 0.05) decrease in blood glucose levels following starch loading (Figure 2(b)). As in the normal rats, 10 mg/kg of acarbose also significantly (P < 0.05) decreased the blood glucose levels of the diabetic rats.


Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 3 g/kg of starch (a). Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663316&req=5

fig2: The effect of oral administration of 50% ethanol extract of O. stamineus at doses of 250, 500, and 1000 mg/kg on blood glucose levels of normal rats (a) and diabetic rats (b) loaded with 3 g/kg of starch (a). Values are expressed as mean ± SEM (n = 6); ∗P < 0.05 and ∗P < 0.01 compared with the control.
Mentions: Using normal rats, extract-treated groups receiving 250 mg/kg and 500 mg/kg of the 50% ethanol extract of O. stamineus did not show any significant decrease in blood glucose levels (Figure 2(a)) compared to control rats. Only a dose of 1000 mg/kg of the extract reduced the blood glucose levels significantly (P < 0.05) after starch loading. Administered at a dose of 10 mg/kg, acarbose also significantly (P < 0.05) reduced the blood glucose levels of starch loaded normal rats. Likewise, using diabetic rats, only the group treated with 1000 mg/kg showed significant (P < 0.05) decrease in blood glucose levels following starch loading (Figure 2(b)). As in the normal rats, 10 mg/kg of acarbose also significantly (P < 0.05) decreased the blood glucose levels of the diabetic rats.

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus