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Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatogram of standard markers and 50% ethanolic extract of O. stamineus. Standard compounds: TMF (3′-hydroxy-5,6,7,4′-tetramethoxyflavone), SEN (sinensetin), and EUP (eupatorin) (red chromatogram). HPLC chromatogram of 50% ethanolic extract of O. stamineus (black chromatogram).
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fig1: HPLC chromatogram of standard markers and 50% ethanolic extract of O. stamineus. Standard compounds: TMF (3′-hydroxy-5,6,7,4′-tetramethoxyflavone), SEN (sinensetin), and EUP (eupatorin) (red chromatogram). HPLC chromatogram of 50% ethanolic extract of O. stamineus (black chromatogram).

Mentions: HPLC analysis showed the percentages of 3′hydroxy-5,6,7,4′-tetramethoxyflavone, sinensetin, and eupatorin in the 50% ethanol extract of O. stamineus to be 1.02 ± 0.0007, 3.76 ± 0.02, and 3.032 ± 0.03, respectively (Figure 1).


Evaluation of α-Glucosidase Inhibitory Effect of 50% Ethanolic Standardized Extract of Orthosiphon stamineus Benth in Normal and Streptozotocin-Induced Diabetic Rats.

Mohamed EA, Ahmad M, Ang LF, Asmawi MZ, Yam MF - Evid Based Complement Alternat Med (2015)

HPLC chromatogram of standard markers and 50% ethanolic extract of O. stamineus. Standard compounds: TMF (3′-hydroxy-5,6,7,4′-tetramethoxyflavone), SEN (sinensetin), and EUP (eupatorin) (red chromatogram). HPLC chromatogram of 50% ethanolic extract of O. stamineus (black chromatogram).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663316&req=5

fig1: HPLC chromatogram of standard markers and 50% ethanolic extract of O. stamineus. Standard compounds: TMF (3′-hydroxy-5,6,7,4′-tetramethoxyflavone), SEN (sinensetin), and EUP (eupatorin) (red chromatogram). HPLC chromatogram of 50% ethanolic extract of O. stamineus (black chromatogram).
Mentions: HPLC analysis showed the percentages of 3′hydroxy-5,6,7,4′-tetramethoxyflavone, sinensetin, and eupatorin in the 50% ethanol extract of O. stamineus to be 1.02 ± 0.0007, 3.76 ± 0.02, and 3.032 ± 0.03, respectively (Figure 1).

Bottom Line: The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose.Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect.Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

ABSTRACT
In the present study, a 50% ethanolic extract of Orthosiphon stamineus was tested for its α-glucosidase inhibitory activity. In vivo assays of the extract (containing 1.02%, 3.76%, and 3.03% of 3'hydroxy-5,6,7,4'-tetramethoxyflavone, sinensetin, and eupatorin, resp.) showed that it possessed an inhibitory activity against α-glucosidase in normal rats loaded with starch and sucrose. The results showed that 1000 mg/kg of the 50% ethanolic extract of O. stamineus significantly (P < 0.05) decreased the plasma glucose levels of the experimental animals in a manner resembling the effect of acarbose. In streptozotocin-induced diabetic rats, only the group treated with 1000 mg/kg of the extract showed significantly (P < 0.05) lower plasma glucose levels after starch loading. Hence, α-glucosidase inhibition might be one of the mechanisms by which O. stamineus extract exerts its antidiabetic effect. Furthermore, our findings indicated that the 50% ethanolic extract of O. stamineus can be considered as a potential agent for the management of diabetes mellitus.

No MeSH data available.


Related in: MedlinePlus