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Comparative Scanning Electron Microscopic Study of the Marginal Adaptation of Four Root-End Filling Materials in Presence and Absence of Blood.

Bolhari B, Ashofteh Yazdi K, Sharifi F, Pirmoazen S - J Dent (Tehran) (2015)

Bottom Line: The Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis of data with P<0.05 as the limit of significance.There were no significant differences in marginal adaptation of the 8 tested groups (P>0.05).Based on the results, blood contamination does not affect the marginal adaptation of MTA, CEM cement, Biodentine or BioAggregate.

View Article: PubMed Central - PubMed

Affiliation: Associate Professor, Dental Research Center, Dentistry Research Institute, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: The aim of this study was to evaluate marginal adaptation of mineral trioxide aggregate (MTA), calcium enriched mixture (CEM) cement, Biodentine and BioAggregate in presence of normal saline and human blood.

Materials and methods: In this in-vitro experimental study, 80 extracted single-rooted human teeth were instrumented and filled with gutta-percha. After resecting the root-end, apical cavity preparation was done and the teeth were randomly divided into 4 groups (N=20)(a total of 8 subgroups). Root-end filling materials were placed in 3mm root-end cavities prepared ultrasonically. Half the specimens in each group were exposed to normal saline and the other half to fresh whole human blood. After 4 days, epoxy resin replicas of the apical portion of samples were fabricated and scanning electron microscopy (SEM) analysis was performed to find gaps in the adaptation of the root-end filling materials at their interface with dentin. The Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis of data with P<0.05 as the limit of significance.

Results: There were no significant differences in marginal adaptation of the 8 tested groups (P>0.05).

Conclusion: Based on the results, blood contamination does not affect the marginal adaptation of MTA, CEM cement, Biodentine or BioAggregate.

No MeSH data available.


Related in: MedlinePlus

Micrograph of root dentin-BioAggregate interface exposed to normal saline (× 200)Micrograph of root dentin-BioAggregate interface exposed to human blood (×200)Micrograph of root dentin-Biodentine interface exposed to normal saline (×200)Micrograph of root dentin-Biodentine interface exposed to human blood (×200)Micrograph of root dentin-CEM interface exposed to normal saline (×200)Micrograph of root dentine-CEM interface exposed to human blood (×200)Micrograph of root dentine-MTA interface exposed to normal saline (×200)Micrograph of root dentine-MTA interface exposed to human blood (×200)
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Figure 1: Micrograph of root dentin-BioAggregate interface exposed to normal saline (× 200)Micrograph of root dentin-BioAggregate interface exposed to human blood (×200)Micrograph of root dentin-Biodentine interface exposed to normal saline (×200)Micrograph of root dentin-Biodentine interface exposed to human blood (×200)Micrograph of root dentin-CEM interface exposed to normal saline (×200)Micrograph of root dentine-CEM interface exposed to human blood (×200)Micrograph of root dentine-MTA interface exposed to normal saline (×200)Micrograph of root dentine-MTA interface exposed to human blood (×200)

Mentions: The mean value and standard deviation (SD) of the marginal gap in each group are summarized in Table 1. There was no significant difference in the measured marginal gap between the 4 filling materials (P>0.05). There was no significant difference between the effect of normal saline and blood on the marginal adaptation of the 4 different filling materials either (P>0.05) (Figure 1). In general, the difference between the measured marginal gap in all 8 groups was not significant (P>0.05).


Comparative Scanning Electron Microscopic Study of the Marginal Adaptation of Four Root-End Filling Materials in Presence and Absence of Blood.

Bolhari B, Ashofteh Yazdi K, Sharifi F, Pirmoazen S - J Dent (Tehran) (2015)

Micrograph of root dentin-BioAggregate interface exposed to normal saline (× 200)Micrograph of root dentin-BioAggregate interface exposed to human blood (×200)Micrograph of root dentin-Biodentine interface exposed to normal saline (×200)Micrograph of root dentin-Biodentine interface exposed to human blood (×200)Micrograph of root dentin-CEM interface exposed to normal saline (×200)Micrograph of root dentine-CEM interface exposed to human blood (×200)Micrograph of root dentine-MTA interface exposed to normal saline (×200)Micrograph of root dentine-MTA interface exposed to human blood (×200)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4663313&req=5

Figure 1: Micrograph of root dentin-BioAggregate interface exposed to normal saline (× 200)Micrograph of root dentin-BioAggregate interface exposed to human blood (×200)Micrograph of root dentin-Biodentine interface exposed to normal saline (×200)Micrograph of root dentin-Biodentine interface exposed to human blood (×200)Micrograph of root dentin-CEM interface exposed to normal saline (×200)Micrograph of root dentine-CEM interface exposed to human blood (×200)Micrograph of root dentine-MTA interface exposed to normal saline (×200)Micrograph of root dentine-MTA interface exposed to human blood (×200)
Mentions: The mean value and standard deviation (SD) of the marginal gap in each group are summarized in Table 1. There was no significant difference in the measured marginal gap between the 4 filling materials (P>0.05). There was no significant difference between the effect of normal saline and blood on the marginal adaptation of the 4 different filling materials either (P>0.05) (Figure 1). In general, the difference between the measured marginal gap in all 8 groups was not significant (P>0.05).

Bottom Line: The Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis of data with P<0.05 as the limit of significance.There were no significant differences in marginal adaptation of the 8 tested groups (P>0.05).Based on the results, blood contamination does not affect the marginal adaptation of MTA, CEM cement, Biodentine or BioAggregate.

View Article: PubMed Central - PubMed

Affiliation: Associate Professor, Dental Research Center, Dentistry Research Institute, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: The aim of this study was to evaluate marginal adaptation of mineral trioxide aggregate (MTA), calcium enriched mixture (CEM) cement, Biodentine and BioAggregate in presence of normal saline and human blood.

Materials and methods: In this in-vitro experimental study, 80 extracted single-rooted human teeth were instrumented and filled with gutta-percha. After resecting the root-end, apical cavity preparation was done and the teeth were randomly divided into 4 groups (N=20)(a total of 8 subgroups). Root-end filling materials were placed in 3mm root-end cavities prepared ultrasonically. Half the specimens in each group were exposed to normal saline and the other half to fresh whole human blood. After 4 days, epoxy resin replicas of the apical portion of samples were fabricated and scanning electron microscopy (SEM) analysis was performed to find gaps in the adaptation of the root-end filling materials at their interface with dentin. The Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis of data with P<0.05 as the limit of significance.

Results: There were no significant differences in marginal adaptation of the 8 tested groups (P>0.05).

Conclusion: Based on the results, blood contamination does not affect the marginal adaptation of MTA, CEM cement, Biodentine or BioAggregate.

No MeSH data available.


Related in: MedlinePlus