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Substrate Type and Free Ammonia Determine Bacterial Community Structure in Full-Scale Mesophilic Anaerobic Digesters Treating Cattle or Swine Manure.

Li J, Rui J, Yao M, Zhang S, Yan X, Wang Y, Yan Z, Li X - Front Microbiol (2015)

Bottom Line: Syntrophic metabolism by Proteobacteria, Chloroflexi, Synergistetes and Planctomycetes are likely inhibited when FA content is high.The core communities were best characterized by phylum Firmicutes, wherein Clostridium predominated overwhelmingly.These findings extend our current understanding of the bacterial assembly in full-scale manure anaerobic digesters.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences Sichuan, China ; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences Sichuan, China.

ABSTRACT
The microbial-mediated anaerobic digestion (AD) process represents an efficient biological process for the treatment of organic waste along with biogas harvest. Currently, the key factors structuring bacterial communities and the potential core and unique bacterial populations in manure anaerobic digesters are not completely elucidated yet. In this study, we collected sludge samples from 20 full-scale anaerobic digesters treating cattle or swine manure, and investigated the variations of bacterial community compositions using high-throughput 16S rRNA amplicon sequencing. Clustering and correlation analysis suggested that substrate type and free ammonia (FA) play key roles in determining the bacterial community structure. The COD: [Formula: see text] (C:N) ratio of substrate and FA were the most important available operational parameters correlating to the bacterial communities in cattle and swine manure digesters, respectively. The bacterial populations in all of the digesters were dominated by phylum Firmicutes, followed by Bacteroidetes, Proteobacteria and Chloroflexi. Increased FA content selected Firmicutes, suggesting that they probably play more important roles under high FA content. Syntrophic metabolism by Proteobacteria, Chloroflexi, Synergistetes and Planctomycetes are likely inhibited when FA content is high. Despite the different manure substrates, operational conditions and geographical locations of digesters, core bacterial communities were identified. The core communities were best characterized by phylum Firmicutes, wherein Clostridium predominated overwhelmingly. Substrate-unique and abundant communities may reflect the properties of manure substrate and operational conditions. These findings extend our current understanding of the bacterial assembly in full-scale manure anaerobic digesters.

No MeSH data available.


Related in: MedlinePlus

The heatmap showing the relative abundances of abundant genera (>0.3%) across all the digesters. c, cattle manure digesters; s, swine manure digesters.
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Figure 3: The heatmap showing the relative abundances of abundant genera (>0.3%) across all the digesters. c, cattle manure digesters; s, swine manure digesters.

Mentions: At the genus level, most abundant genera (relative abundance >0.30%) were affiliated to phylum Firmicutes, e.g., Clostridium sensu stricto, Clostridium XI, Syntrophomonas, Clostridium_III, and Pelotomaculum (Figure 3 and Table S3). Other abundant genera were also included, such as Smithella, Syntrophorhabdus in phylum Proteobacteria, and Corynebacterium in phylum Actinobacteria. The 19 genera accounted for 40.4% of total genera reads. Despite the dominance of these genera, high variations in the relative abundance were observed in different samples, e.g., Clostridium sensu stricto varied between 0.14 and 56.75%, and Clostridium XI between 0.89 and 52.15% (Table S3).


Substrate Type and Free Ammonia Determine Bacterial Community Structure in Full-Scale Mesophilic Anaerobic Digesters Treating Cattle or Swine Manure.

Li J, Rui J, Yao M, Zhang S, Yan X, Wang Y, Yan Z, Li X - Front Microbiol (2015)

The heatmap showing the relative abundances of abundant genera (>0.3%) across all the digesters. c, cattle manure digesters; s, swine manure digesters.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663275&req=5

Figure 3: The heatmap showing the relative abundances of abundant genera (>0.3%) across all the digesters. c, cattle manure digesters; s, swine manure digesters.
Mentions: At the genus level, most abundant genera (relative abundance >0.30%) were affiliated to phylum Firmicutes, e.g., Clostridium sensu stricto, Clostridium XI, Syntrophomonas, Clostridium_III, and Pelotomaculum (Figure 3 and Table S3). Other abundant genera were also included, such as Smithella, Syntrophorhabdus in phylum Proteobacteria, and Corynebacterium in phylum Actinobacteria. The 19 genera accounted for 40.4% of total genera reads. Despite the dominance of these genera, high variations in the relative abundance were observed in different samples, e.g., Clostridium sensu stricto varied between 0.14 and 56.75%, and Clostridium XI between 0.89 and 52.15% (Table S3).

Bottom Line: Syntrophic metabolism by Proteobacteria, Chloroflexi, Synergistetes and Planctomycetes are likely inhibited when FA content is high.The core communities were best characterized by phylum Firmicutes, wherein Clostridium predominated overwhelmingly.These findings extend our current understanding of the bacterial assembly in full-scale manure anaerobic digesters.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences Sichuan, China ; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences Sichuan, China.

ABSTRACT
The microbial-mediated anaerobic digestion (AD) process represents an efficient biological process for the treatment of organic waste along with biogas harvest. Currently, the key factors structuring bacterial communities and the potential core and unique bacterial populations in manure anaerobic digesters are not completely elucidated yet. In this study, we collected sludge samples from 20 full-scale anaerobic digesters treating cattle or swine manure, and investigated the variations of bacterial community compositions using high-throughput 16S rRNA amplicon sequencing. Clustering and correlation analysis suggested that substrate type and free ammonia (FA) play key roles in determining the bacterial community structure. The COD: [Formula: see text] (C:N) ratio of substrate and FA were the most important available operational parameters correlating to the bacterial communities in cattle and swine manure digesters, respectively. The bacterial populations in all of the digesters were dominated by phylum Firmicutes, followed by Bacteroidetes, Proteobacteria and Chloroflexi. Increased FA content selected Firmicutes, suggesting that they probably play more important roles under high FA content. Syntrophic metabolism by Proteobacteria, Chloroflexi, Synergistetes and Planctomycetes are likely inhibited when FA content is high. Despite the different manure substrates, operational conditions and geographical locations of digesters, core bacterial communities were identified. The core communities were best characterized by phylum Firmicutes, wherein Clostridium predominated overwhelmingly. Substrate-unique and abundant communities may reflect the properties of manure substrate and operational conditions. These findings extend our current understanding of the bacterial assembly in full-scale manure anaerobic digesters.

No MeSH data available.


Related in: MedlinePlus