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Actinobacterial Diversity in the Sediments of Five Cold Springs on the Qinghai-Tibet Plateau.

Yang J, Li X, Huang L, Jiang H - Front Microbiol (2015)

Bottom Line: The actinobacterial composition varied among the investigated cold springs and were significantly correlated (r = 0.748, P = 0.021) to environmental variables.The actinobacterial communities in the cold springs were more diverse than other cold habitats on the Tibetan Plateau, and their compositions showed unique geographical distribution characteristics.Statistical analyses showed that biogeographical isolation and unique environmental conditions might be major factors influencing actinobacterial distribution among the investigated cold springs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China.

ABSTRACT
The actinobacterial diversity was investigated in the sediments of five cold springs in Wuli region on the Qinghai-Tibet Plateau using 16S rRNA gene phylogenetic analysis. The actinobacterial communities of the studied cold springs were diverse and the obtained actinobacterial operational taxonomic units were classified into 12 actinobacterial orders (e.g., Acidimicrobiales, Corynebacteriales, Gaiellales, Geodermatophilales, Jiangellales, Kineosporiales, Micromonosporales, Micrococcales, Nakamurellales, Propionibacteriales, Pseudonocardiales, Streptomycetales) and unclassified Actinobacteria. The actinobacterial composition varied among the investigated cold springs and were significantly correlated (r = 0.748, P = 0.021) to environmental variables. The actinobacterial communities in the cold springs were more diverse than other cold habitats on the Tibetan Plateau, and their compositions showed unique geographical distribution characteristics. Statistical analyses showed that biogeographical isolation and unique environmental conditions might be major factors influencing actinobacterial distribution among the investigated cold springs.

No MeSH data available.


Related in: MedlinePlus

A geographic map showing the locations of sampling sites in Wuli County, Qinghai-Tibet Plateau, China.
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Figure 1: A geographic map showing the locations of sampling sites in Wuli County, Qinghai-Tibet Plateau, China.

Mentions: In July 2010, five cold springs were sampled in Wuli Area (Figure 1), Qinghai Province, China, that is adjacent to the Daha coal mine (Zhou, 2004) and located in the Fenghuo Mountain-Wuli gas hydrate zone (Zhu et al., 2011). The Wuli area is located at the elevation of ∼4600 m. Water pH and temperature were measured in the field using a digital soil pH meter (Ferrymorse-Seed Company) and a mercury thermometer, respectively. During sample collection (around noon), the ambient temperature was 15–17°C, whereas the water temperature of the sampled cold springs was around 1–3°C. Sediments from five cold springs (named as QCS1, QCS3, QCS4, QCS5, and QCS6, respectively) were collected into 50 mL sterile Falcon tubes using a sterile spatula. The collected samples were stored at -20°C in the field as well as during transportation and subsequently at -80°C in the laboratory until further analyses.


Actinobacterial Diversity in the Sediments of Five Cold Springs on the Qinghai-Tibet Plateau.

Yang J, Li X, Huang L, Jiang H - Front Microbiol (2015)

A geographic map showing the locations of sampling sites in Wuli County, Qinghai-Tibet Plateau, China.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4663260&req=5

Figure 1: A geographic map showing the locations of sampling sites in Wuli County, Qinghai-Tibet Plateau, China.
Mentions: In July 2010, five cold springs were sampled in Wuli Area (Figure 1), Qinghai Province, China, that is adjacent to the Daha coal mine (Zhou, 2004) and located in the Fenghuo Mountain-Wuli gas hydrate zone (Zhu et al., 2011). The Wuli area is located at the elevation of ∼4600 m. Water pH and temperature were measured in the field using a digital soil pH meter (Ferrymorse-Seed Company) and a mercury thermometer, respectively. During sample collection (around noon), the ambient temperature was 15–17°C, whereas the water temperature of the sampled cold springs was around 1–3°C. Sediments from five cold springs (named as QCS1, QCS3, QCS4, QCS5, and QCS6, respectively) were collected into 50 mL sterile Falcon tubes using a sterile spatula. The collected samples were stored at -20°C in the field as well as during transportation and subsequently at -80°C in the laboratory until further analyses.

Bottom Line: The actinobacterial composition varied among the investigated cold springs and were significantly correlated (r = 0.748, P = 0.021) to environmental variables.The actinobacterial communities in the cold springs were more diverse than other cold habitats on the Tibetan Plateau, and their compositions showed unique geographical distribution characteristics.Statistical analyses showed that biogeographical isolation and unique environmental conditions might be major factors influencing actinobacterial distribution among the investigated cold springs.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan, China.

ABSTRACT
The actinobacterial diversity was investigated in the sediments of five cold springs in Wuli region on the Qinghai-Tibet Plateau using 16S rRNA gene phylogenetic analysis. The actinobacterial communities of the studied cold springs were diverse and the obtained actinobacterial operational taxonomic units were classified into 12 actinobacterial orders (e.g., Acidimicrobiales, Corynebacteriales, Gaiellales, Geodermatophilales, Jiangellales, Kineosporiales, Micromonosporales, Micrococcales, Nakamurellales, Propionibacteriales, Pseudonocardiales, Streptomycetales) and unclassified Actinobacteria. The actinobacterial composition varied among the investigated cold springs and were significantly correlated (r = 0.748, P = 0.021) to environmental variables. The actinobacterial communities in the cold springs were more diverse than other cold habitats on the Tibetan Plateau, and their compositions showed unique geographical distribution characteristics. Statistical analyses showed that biogeographical isolation and unique environmental conditions might be major factors influencing actinobacterial distribution among the investigated cold springs.

No MeSH data available.


Related in: MedlinePlus