Limits...
The expression and activity of thioredoxin reductase 1 splice variants v1 and v2 regulate the expression of genes associated with differentiation and adhesion.

Nalvarte I, Damdimopoulos AE, Rüegg J, Spyrou G - Biosci. Rep. (2015)

Bottom Line: The mammalian redox-active selenoprotein thioredoxin reductase (TrxR1) is a main player in redox homoeostasis.Overexpression of these two splice forms resulted in distinctive effects on various aspects of cellular functions including gene regulation patterns, alteration of growth rate, migration and morphology and susceptibility to selenium-induced toxicity.These data suggest that both TXNRD1_v1 and TXNRD1_v2 have distinct roles in differentiation, possibly by altering the expression of the genes associated with differentiation, and further emphasize the importance in distinguishing each unique action of different TrxR1 splice forms, especially when studying the gene silencing or knockout of TrxR1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Nutrition, Karolinska Institute, SE-141 83 Huddinge, Sweden ivan.nalvarte@ki.se.

No MeSH data available.


Related in: MedlinePlus

Growth characteristics of HEK-293 cells overexpressing TXNRD1v_1 and TXNRD1_v2The cells were grown in plain medium, 0.2, 1, 5 or 10 μM selenium-supplemented media for the indicated days. Cells were counted using Trypan Blue exclusion. Error bars correspond to S.E.M. of three biological replicates counted in triplicates, *P<0.05, **P<0.01, ***P<0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4660583&req=5

Figure 2: Growth characteristics of HEK-293 cells overexpressing TXNRD1v_1 and TXNRD1_v2The cells were grown in plain medium, 0.2, 1, 5 or 10 μM selenium-supplemented media for the indicated days. Cells were counted using Trypan Blue exclusion. Error bars correspond to S.E.M. of three biological replicates counted in triplicates, *P<0.05, **P<0.01, ***P<0.001.

Mentions: As reported earlier, we found that TXNRD1_v1 overexpression caused HEK-293 cells to grow slower and to have a higher resistance towards selenium-induced toxicity [17,26]. However, although TXNRD1_v2 overexpression also resulted in slower growth, it was not as protective against selenium toxicity as TXNRD1_v1 at 10 μM Se (Figure 2). This could, as above, possibly be explained by the generally lower expression of TXNRD1_v2 and/or poorer incorporation of selenium into the protein.


The expression and activity of thioredoxin reductase 1 splice variants v1 and v2 regulate the expression of genes associated with differentiation and adhesion.

Nalvarte I, Damdimopoulos AE, Rüegg J, Spyrou G - Biosci. Rep. (2015)

Growth characteristics of HEK-293 cells overexpressing TXNRD1v_1 and TXNRD1_v2The cells were grown in plain medium, 0.2, 1, 5 or 10 μM selenium-supplemented media for the indicated days. Cells were counted using Trypan Blue exclusion. Error bars correspond to S.E.M. of three biological replicates counted in triplicates, *P<0.05, **P<0.01, ***P<0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660583&req=5

Figure 2: Growth characteristics of HEK-293 cells overexpressing TXNRD1v_1 and TXNRD1_v2The cells were grown in plain medium, 0.2, 1, 5 or 10 μM selenium-supplemented media for the indicated days. Cells were counted using Trypan Blue exclusion. Error bars correspond to S.E.M. of three biological replicates counted in triplicates, *P<0.05, **P<0.01, ***P<0.001.
Mentions: As reported earlier, we found that TXNRD1_v1 overexpression caused HEK-293 cells to grow slower and to have a higher resistance towards selenium-induced toxicity [17,26]. However, although TXNRD1_v2 overexpression also resulted in slower growth, it was not as protective against selenium toxicity as TXNRD1_v1 at 10 μM Se (Figure 2). This could, as above, possibly be explained by the generally lower expression of TXNRD1_v2 and/or poorer incorporation of selenium into the protein.

Bottom Line: The mammalian redox-active selenoprotein thioredoxin reductase (TrxR1) is a main player in redox homoeostasis.Overexpression of these two splice forms resulted in distinctive effects on various aspects of cellular functions including gene regulation patterns, alteration of growth rate, migration and morphology and susceptibility to selenium-induced toxicity.These data suggest that both TXNRD1_v1 and TXNRD1_v2 have distinct roles in differentiation, possibly by altering the expression of the genes associated with differentiation, and further emphasize the importance in distinguishing each unique action of different TrxR1 splice forms, especially when studying the gene silencing or knockout of TrxR1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosciences and Nutrition, Karolinska Institute, SE-141 83 Huddinge, Sweden ivan.nalvarte@ki.se.

No MeSH data available.


Related in: MedlinePlus