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The mitotic regulator Hec1 is a critical modulator of prostate cancer through the long non-coding RNA BX647187 in vitro.

Wang H, Gao X, Lu X, Wang Y, Ma C, Shi Z, Zhu F, He B, Xu C, Sun Y - Biosci. Rep. (2015)

Bottom Line: Through bioinformatics analysis and knockdown Hec1 in PCa cells, we found LncRNA BX647187 was positively regulated by Hec1.We further demonstrated that suppression of BX647187 in PCa cells significantly reduced cell proliferation and promoted apoptosis.Thus, we conclude that Hec1 is consistently overexpressed in human PCa and Hec1 is closely linked with human PCa progression through the meditator LncRNA BX647187.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.

No MeSH data available.


Related in: MedlinePlus

LncRNA expression profiles between PCa tissue and adjacent non-tumour samples(A) Heat map analysis of the LncRNAs expression of groups was created using a method of hierarchical clustering by GeneSpring GX, version 7.3. Rows: samples; columns: LncRNAs; colour key indicates LncRNA expression value, red: highest, blue: lowest. Microarray data obtained from Gene Expression Omnibus (GEO), GSE number is GSE3325. (B) Six biggest up-regulated LncRNAs in PCa tissue were further validated for altered transcription level using qRT-PCR. PSA is used as a positive control for PCa tissue. The relative amount of each LncRNAs was normalized to GAPDH. Dates in histograms are means ± S.D., *P<0.05, **P<0.01compared with normal group (ttest).
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Figure 3: LncRNA expression profiles between PCa tissue and adjacent non-tumour samples(A) Heat map analysis of the LncRNAs expression of groups was created using a method of hierarchical clustering by GeneSpring GX, version 7.3. Rows: samples; columns: LncRNAs; colour key indicates LncRNA expression value, red: highest, blue: lowest. Microarray data obtained from Gene Expression Omnibus (GEO), GSE number is GSE3325. (B) Six biggest up-regulated LncRNAs in PCa tissue were further validated for altered transcription level using qRT-PCR. PSA is used as a positive control for PCa tissue. The relative amount of each LncRNAs was normalized to GAPDH. Dates in histograms are means ± S.D., *P<0.05, **P<0.01compared with normal group (ttest).

Mentions: We used the microarray data GSE3325, submitted to the Gene Expression Omnibus (GEO) by Varambally at September 17, 2005 and last update at November 26, 2014, to analyse differential LncRNAs expression between PCa tissue and adjacent non-tumour samples. Firstly, hierarchical clustering analysis was used to compare differential LncRNA expression of the top 50 LncRNAs. The clustered heat map of 50 LncRNAs for GSE3325 is shown in Figure 3(A). Then, we further confirmed the selected six up-regulation of LncRNAs in PCa tissues and PCa cell lines through qRT-PCR. As shown in Figure 3(B), comparing with 293T cells, the expression of LncRNA BX647187 was significantly increased ~10-fold in PC3 cells, 8-fold in LNCap cells and 6.5-fold in Du145 cells. And all other five LncRNAs have a certain degree of up-regulation in PCa cell lines. In cancerous tissues, BX647187 expression was also at a level significantly higher than the average level of normal specimens (Figure 3C).


The mitotic regulator Hec1 is a critical modulator of prostate cancer through the long non-coding RNA BX647187 in vitro.

Wang H, Gao X, Lu X, Wang Y, Ma C, Shi Z, Zhu F, He B, Xu C, Sun Y - Biosci. Rep. (2015)

LncRNA expression profiles between PCa tissue and adjacent non-tumour samples(A) Heat map analysis of the LncRNAs expression of groups was created using a method of hierarchical clustering by GeneSpring GX, version 7.3. Rows: samples; columns: LncRNAs; colour key indicates LncRNA expression value, red: highest, blue: lowest. Microarray data obtained from Gene Expression Omnibus (GEO), GSE number is GSE3325. (B) Six biggest up-regulated LncRNAs in PCa tissue were further validated for altered transcription level using qRT-PCR. PSA is used as a positive control for PCa tissue. The relative amount of each LncRNAs was normalized to GAPDH. Dates in histograms are means ± S.D., *P<0.05, **P<0.01compared with normal group (ttest).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 3: LncRNA expression profiles between PCa tissue and adjacent non-tumour samples(A) Heat map analysis of the LncRNAs expression of groups was created using a method of hierarchical clustering by GeneSpring GX, version 7.3. Rows: samples; columns: LncRNAs; colour key indicates LncRNA expression value, red: highest, blue: lowest. Microarray data obtained from Gene Expression Omnibus (GEO), GSE number is GSE3325. (B) Six biggest up-regulated LncRNAs in PCa tissue were further validated for altered transcription level using qRT-PCR. PSA is used as a positive control for PCa tissue. The relative amount of each LncRNAs was normalized to GAPDH. Dates in histograms are means ± S.D., *P<0.05, **P<0.01compared with normal group (ttest).
Mentions: We used the microarray data GSE3325, submitted to the Gene Expression Omnibus (GEO) by Varambally at September 17, 2005 and last update at November 26, 2014, to analyse differential LncRNAs expression between PCa tissue and adjacent non-tumour samples. Firstly, hierarchical clustering analysis was used to compare differential LncRNA expression of the top 50 LncRNAs. The clustered heat map of 50 LncRNAs for GSE3325 is shown in Figure 3(A). Then, we further confirmed the selected six up-regulation of LncRNAs in PCa tissues and PCa cell lines through qRT-PCR. As shown in Figure 3(B), comparing with 293T cells, the expression of LncRNA BX647187 was significantly increased ~10-fold in PC3 cells, 8-fold in LNCap cells and 6.5-fold in Du145 cells. And all other five LncRNAs have a certain degree of up-regulation in PCa cell lines. In cancerous tissues, BX647187 expression was also at a level significantly higher than the average level of normal specimens (Figure 3C).

Bottom Line: Through bioinformatics analysis and knockdown Hec1 in PCa cells, we found LncRNA BX647187 was positively regulated by Hec1.We further demonstrated that suppression of BX647187 in PCa cells significantly reduced cell proliferation and promoted apoptosis.Thus, we conclude that Hec1 is consistently overexpressed in human PCa and Hec1 is closely linked with human PCa progression through the meditator LncRNA BX647187.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Changhai Hospital, Second Military University, Shanghai, 20043, China.

No MeSH data available.


Related in: MedlinePlus