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The evaluation of the activity of medicinal remedies of plant and animal origin on the regeneration of the earthworms' tail segments.

Bybin VA, Stom DI - Pharmacognosy Res (2014 Oct-Dec)

Bottom Line: The new individual can be fully recovered only from the front half of the body in many species of earthworms.The relations of the activity of preparations tested with doses and the time of the storage have been revealed.The principal possibility of applicability of the test reaction studied as a way to evaluate the effects and quality of remedies based on medicinal plants and earthworms has been shown.

View Article: PubMed Central - PubMed

Affiliation: Federal State Budgetary Educational Establishment of the Higher Professional Education, "Irkutsk State University", Scientific Research Institute of Biology, Russian Federation, Russia.

ABSTRACT
Now, in the global community there is enough hard recommendation to replace the vertebrate test animals into simpler organisms at the development, testing, and evaluation of the quality pharmaceuticals. The feature of planarian to regenerate in new individual planarian from a piece, which is only 1/7 of the original animal, allowed to create the alternative methods of testing of drugs, dietary supplements, water quality, influence of electromagnetic fields, and other radiations. The tests on planarian can replace the ones that are held today on mammals. However, the lacks of the bioassays based on the planarian regeneration are the need for complex and expensive video equipment for recording the regrowth of worms' body, the difficulties of culturing of flatworms and fairly long period of response. These difficulties can be avoided by using another group of the worms of type Annelida. The new individual can be fully recovered only from the front half of the body in many species of earthworms. Thus, the influence of the pharmaceuticals from earthworms, mummy, and Orthilia secunda on the ability of earthworms to regenerate lost tail segments has been investigated. The relations of the activity of preparations tested with doses and the time of the storage have been revealed. The principal possibility of applicability of the test reaction studied as a way to evaluate the effects and quality of remedies based on medicinal plants and earthworms has been shown.

No MeSH data available.


Related in: MedlinePlus

The regeneration of the tail segments of Eisenia fetida on the 22 days of exposure of oligochaetes after treatment by: (a) 0.01% solution of the preparation “golden mummy”, (b) 0.1% solution of the preparation “Golden mummy”, (c) 1% solution of the preparation “golden mummies”, (d) physiological saline (control)
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Figure 3: The regeneration of the tail segments of Eisenia fetida on the 22 days of exposure of oligochaetes after treatment by: (a) 0.01% solution of the preparation “golden mummy”, (b) 0.1% solution of the preparation “Golden mummy”, (c) 1% solution of the preparation “golden mummies”, (d) physiological saline (control)

Mentions: The increasing of the concentrations of intact vermipreparation to 0.1% led to a suppression of regeneration on average 56% (td= 3.1; P ≤ 0.05) [Figure 1a], while animals were insensitive to the vermipreparation attenuated by heating in the same concentration [Figure 1b]. A further increase in the concentration of both types of vermipreparations to 0.5% and 1% did not significantly alter the rate of the segment's regeneration. The preparation “golden mummy” inhibited regeneration of earthworms at 0.1% and 1% on average by 32% and 50% (td= 2.6, P ≤ 0.05; td= 4.2, P ≤ 0.05), respectively and stimulated on 112% (td= 6.2; P ≤ 0.05) with content of the mummy 0.01% compared to the control [Figures 2 and 3].


The evaluation of the activity of medicinal remedies of plant and animal origin on the regeneration of the earthworms' tail segments.

Bybin VA, Stom DI - Pharmacognosy Res (2014 Oct-Dec)

The regeneration of the tail segments of Eisenia fetida on the 22 days of exposure of oligochaetes after treatment by: (a) 0.01% solution of the preparation “golden mummy”, (b) 0.1% solution of the preparation “Golden mummy”, (c) 1% solution of the preparation “golden mummies”, (d) physiological saline (control)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660520&req=5

Figure 3: The regeneration of the tail segments of Eisenia fetida on the 22 days of exposure of oligochaetes after treatment by: (a) 0.01% solution of the preparation “golden mummy”, (b) 0.1% solution of the preparation “Golden mummy”, (c) 1% solution of the preparation “golden mummies”, (d) physiological saline (control)
Mentions: The increasing of the concentrations of intact vermipreparation to 0.1% led to a suppression of regeneration on average 56% (td= 3.1; P ≤ 0.05) [Figure 1a], while animals were insensitive to the vermipreparation attenuated by heating in the same concentration [Figure 1b]. A further increase in the concentration of both types of vermipreparations to 0.5% and 1% did not significantly alter the rate of the segment's regeneration. The preparation “golden mummy” inhibited regeneration of earthworms at 0.1% and 1% on average by 32% and 50% (td= 2.6, P ≤ 0.05; td= 4.2, P ≤ 0.05), respectively and stimulated on 112% (td= 6.2; P ≤ 0.05) with content of the mummy 0.01% compared to the control [Figures 2 and 3].

Bottom Line: The new individual can be fully recovered only from the front half of the body in many species of earthworms.The relations of the activity of preparations tested with doses and the time of the storage have been revealed.The principal possibility of applicability of the test reaction studied as a way to evaluate the effects and quality of remedies based on medicinal plants and earthworms has been shown.

View Article: PubMed Central - PubMed

Affiliation: Federal State Budgetary Educational Establishment of the Higher Professional Education, "Irkutsk State University", Scientific Research Institute of Biology, Russian Federation, Russia.

ABSTRACT
Now, in the global community there is enough hard recommendation to replace the vertebrate test animals into simpler organisms at the development, testing, and evaluation of the quality pharmaceuticals. The feature of planarian to regenerate in new individual planarian from a piece, which is only 1/7 of the original animal, allowed to create the alternative methods of testing of drugs, dietary supplements, water quality, influence of electromagnetic fields, and other radiations. The tests on planarian can replace the ones that are held today on mammals. However, the lacks of the bioassays based on the planarian regeneration are the need for complex and expensive video equipment for recording the regrowth of worms' body, the difficulties of culturing of flatworms and fairly long period of response. These difficulties can be avoided by using another group of the worms of type Annelida. The new individual can be fully recovered only from the front half of the body in many species of earthworms. Thus, the influence of the pharmaceuticals from earthworms, mummy, and Orthilia secunda on the ability of earthworms to regenerate lost tail segments has been investigated. The relations of the activity of preparations tested with doses and the time of the storage have been revealed. The principal possibility of applicability of the test reaction studied as a way to evaluate the effects and quality of remedies based on medicinal plants and earthworms has been shown.

No MeSH data available.


Related in: MedlinePlus