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Preclinical screening of phyllanthus amarus ethanolic extract for its analgesic and antimicrobial activity.

Bhat SS, Hegde KS, Chandrashekhar S, Rao SN, Manikkoth S - Pharmacognosy Res (2014 Oct-Dec)

Bottom Line: The ethanolic extract of P. amarus showed significant (P < 0.05) peripheral and central analgesic activity.In vitro antimicrobial screening indicated that the ethanolic extract had shown a zone of inhibition against S. mutans and S. salivarius in the agar wells.This study showed that PAEE exhibited significant analgesic and antimicrobial activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Paedodontics and Preventive Dentistry, Yenepoya University, Mangalore, Karnataka, India.

ABSTRACT

Background: To discover a new agent which possesses dual property of analgesic and antimicrobial activity, thereby reducing the burden of polypharmacy. Phyllanthus amarus was screened for its analgesic and antimicrobial activities.

Objectives: The objective was to evaluate the analgesic and antimicrobial activity, of P. amarus ethanolic extract (PAEE).

Materials and methods: The ethanolic extract of P. amarus was prepared using Soxhlet apparatus. An in vivo study using Swiss albino mice was done to screen the central and peripheral analgesic activity of P. amarus extract. The extract was administered at a dose of 100 mg/kg body weight orally. The peripheral analgesic activity was assessed using acetic acid induced writhing test. The central analgesic activity was assessed using Eddy's hot plate apparatus. An in vitro study was carried out to study the antimicrobial activity of the above extract using selected species of Streptococcus mutans, and S. salivarius. The antimicrobial activities were determined using the agar well method.

Results: The ethanolic extract of P. amarus showed significant (P < 0.05) peripheral and central analgesic activity. In vitro antimicrobial screening indicated that the ethanolic extract had shown a zone of inhibition against S. mutans and S. salivarius in the agar wells.

Conclusion: This study showed that PAEE exhibited significant analgesic and antimicrobial activities.

No MeSH data available.


Related in: MedlinePlus

Acetic acid induced writhing reflex
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Figure 3: Acetic acid induced writhing reflex

Mentions: Each group of mice (n = 6), was treated with vehicle (distilled water, 0.1 ml orally), PAEE (100 mg/kg body weight orally) and Ibuprofen (30 mg/kg orally) for 10 days. On 10th day, 1-h after the drug administration, analgesic activity was assessed by counting the number of writhes induced by 0.6% acetic acid administered intraperitoneally. Numbers of writhes per animal was counted for 10 min. A writhe was considered when animal showed contraction of abdomen with simultaneous stretching of at least one hind limb [Figure 3]. Protection against writhing was taken as an index of analgesia.[16]


Preclinical screening of phyllanthus amarus ethanolic extract for its analgesic and antimicrobial activity.

Bhat SS, Hegde KS, Chandrashekhar S, Rao SN, Manikkoth S - Pharmacognosy Res (2014 Oct-Dec)

Acetic acid induced writhing reflex
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660518&req=5

Figure 3: Acetic acid induced writhing reflex
Mentions: Each group of mice (n = 6), was treated with vehicle (distilled water, 0.1 ml orally), PAEE (100 mg/kg body weight orally) and Ibuprofen (30 mg/kg orally) for 10 days. On 10th day, 1-h after the drug administration, analgesic activity was assessed by counting the number of writhes induced by 0.6% acetic acid administered intraperitoneally. Numbers of writhes per animal was counted for 10 min. A writhe was considered when animal showed contraction of abdomen with simultaneous stretching of at least one hind limb [Figure 3]. Protection against writhing was taken as an index of analgesia.[16]

Bottom Line: The ethanolic extract of P. amarus showed significant (P < 0.05) peripheral and central analgesic activity.In vitro antimicrobial screening indicated that the ethanolic extract had shown a zone of inhibition against S. mutans and S. salivarius in the agar wells.This study showed that PAEE exhibited significant analgesic and antimicrobial activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Paedodontics and Preventive Dentistry, Yenepoya University, Mangalore, Karnataka, India.

ABSTRACT

Background: To discover a new agent which possesses dual property of analgesic and antimicrobial activity, thereby reducing the burden of polypharmacy. Phyllanthus amarus was screened for its analgesic and antimicrobial activities.

Objectives: The objective was to evaluate the analgesic and antimicrobial activity, of P. amarus ethanolic extract (PAEE).

Materials and methods: The ethanolic extract of P. amarus was prepared using Soxhlet apparatus. An in vivo study using Swiss albino mice was done to screen the central and peripheral analgesic activity of P. amarus extract. The extract was administered at a dose of 100 mg/kg body weight orally. The peripheral analgesic activity was assessed using acetic acid induced writhing test. The central analgesic activity was assessed using Eddy's hot plate apparatus. An in vitro study was carried out to study the antimicrobial activity of the above extract using selected species of Streptococcus mutans, and S. salivarius. The antimicrobial activities were determined using the agar well method.

Results: The ethanolic extract of P. amarus showed significant (P < 0.05) peripheral and central analgesic activity. In vitro antimicrobial screening indicated that the ethanolic extract had shown a zone of inhibition against S. mutans and S. salivarius in the agar wells.

Conclusion: This study showed that PAEE exhibited significant analgesic and antimicrobial activities.

No MeSH data available.


Related in: MedlinePlus