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Anti-inflammatory and anti-arthritic activity of a methanol extract from Vitellaria paradoxa stem bark.

Foyet HS, Tsala DE, Zogo Essono Bodo JC, Carine AN, Heroyne LT, Oben EK - Pharmacognosy Res (2014 Oct-Dec)

Bottom Line: The effects on hyperalgesia and locomotors activity were also quantified.On the complete Freund's adjuvant-induced rheumatoid arthritis, VPME showed a significant protective effect with 8.12% inflammation against 25.00% for the control group after 2 days of the treatment.The extract (75 and 150 mg/kg) significantly reduced the score of arthritis with a maximum obtained on day 19(th) of the experimentation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Sciences, University of Maroua, P.O Box 814, Cameroon.

ABSTRACT

Background: Vitellaria paradoxa is a traditional medicinal plant of Cameroon. Several studies on this plant have focused on the cosmetic profile of its fruits. The present study focuses on the anti-inflammatory potency of stem barks extract of this plant.

Objective: The objective was to evaluate the effect of methanolic extract of V. paradoxa (VPME) stem barks on inflammatory response in rats.

Materials and methods: Anti-inflammatory effects of VPME were evaluated in acute and chronic (28 days) inflammation induced in Wistar albino rats. The effects on hyperalgesia and locomotors activity were also quantified. The relative weight of lymphoid organs was obtained as well as some hematological parameters.

Results: In the carrageenan-induced inflammation, VPME (75 mg/kg) exhibited a significant (66.67%) inhibition after 1 h. On the complete Freund's adjuvant-induced rheumatoid arthritis, VPME showed a significant protective effect with 8.12% inflammation against 25.00% for the control group after 2 days of the treatment. The extract (75 and 150 mg/kg) significantly reduced the score of arthritis with a maximum obtained on day 19(th) of the experimentation. There was a significant increase in the reaction time of rats on the hot plate as well as the exploratory activities of the animals in the open field. This extract significantly prevented weight, hemoglobin and red blood cells losses, and spleen hypertrophy. A protective action against skin destruction and cartilage erosion was evident. Liquid chromatography-mass spectrometry analysis of the extract revealed the presence of catechins.

Conclusions: These findings suggested that V. paradoxa may contribute to the reduction of the inflammatory response.

No MeSH data available.


Related in: MedlinePlus

Histological structures of joint and cartilage (H and E, ×400) from treated and nontreated rats. The normal control rat (a); the arthritic rat (b); methanol extract of Vitellaria paradoxa treated rat 150 mg/kg (c), 300 mg/kg (d) and diclofenac-treated rat (e). The black arrow (in a-c) showed the joint space variation, the yellow arrow showed the chondrocytes in the cartilage tissue and red arrow showed cartilage erosion
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Figure 10: Histological structures of joint and cartilage (H and E, ×400) from treated and nontreated rats. The normal control rat (a); the arthritic rat (b); methanol extract of Vitellaria paradoxa treated rat 150 mg/kg (c), 300 mg/kg (d) and diclofenac-treated rat (e). The black arrow (in a-c) showed the joint space variation, the yellow arrow showed the chondrocytes in the cartilage tissue and red arrow showed cartilage erosion

Mentions: The histological assessment of tissue sections from rat foot skin and ankles was performed on day 28 of treatment. The histopathology of paw tissue [Figure 9] revealed that adjuvant-induced arthritic rats showed severe edema formation, cells infiltration, vascular dilatation and a disorganization of fibroblasts and collagen fibers. Diclofenac-treated group shows moderate edema formation and cellular infiltration and the VPME-treated groups showed a relative good organization of the skin structure without edema formation and vasodilatation. The joint histological architecture of rats nontreated with CFA was normal [Figure 10a]. This joint architecture was markedly abnormal in the RA model group rats [Figure 10b], which synovial hyperplasia, extensive erosive with an increase of the joint space, chondrocytes destruction, and changes in the cartilage. However, the treatment with the VPME has a protective effect on the joint structure and tends to maintain the normal histological architecture with a chondrocytes multiplication and cartilage protection [Figure 10c-e].


Anti-inflammatory and anti-arthritic activity of a methanol extract from Vitellaria paradoxa stem bark.

Foyet HS, Tsala DE, Zogo Essono Bodo JC, Carine AN, Heroyne LT, Oben EK - Pharmacognosy Res (2014 Oct-Dec)

Histological structures of joint and cartilage (H and E, ×400) from treated and nontreated rats. The normal control rat (a); the arthritic rat (b); methanol extract of Vitellaria paradoxa treated rat 150 mg/kg (c), 300 mg/kg (d) and diclofenac-treated rat (e). The black arrow (in a-c) showed the joint space variation, the yellow arrow showed the chondrocytes in the cartilage tissue and red arrow showed cartilage erosion
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4660517&req=5

Figure 10: Histological structures of joint and cartilage (H and E, ×400) from treated and nontreated rats. The normal control rat (a); the arthritic rat (b); methanol extract of Vitellaria paradoxa treated rat 150 mg/kg (c), 300 mg/kg (d) and diclofenac-treated rat (e). The black arrow (in a-c) showed the joint space variation, the yellow arrow showed the chondrocytes in the cartilage tissue and red arrow showed cartilage erosion
Mentions: The histological assessment of tissue sections from rat foot skin and ankles was performed on day 28 of treatment. The histopathology of paw tissue [Figure 9] revealed that adjuvant-induced arthritic rats showed severe edema formation, cells infiltration, vascular dilatation and a disorganization of fibroblasts and collagen fibers. Diclofenac-treated group shows moderate edema formation and cellular infiltration and the VPME-treated groups showed a relative good organization of the skin structure without edema formation and vasodilatation. The joint histological architecture of rats nontreated with CFA was normal [Figure 10a]. This joint architecture was markedly abnormal in the RA model group rats [Figure 10b], which synovial hyperplasia, extensive erosive with an increase of the joint space, chondrocytes destruction, and changes in the cartilage. However, the treatment with the VPME has a protective effect on the joint structure and tends to maintain the normal histological architecture with a chondrocytes multiplication and cartilage protection [Figure 10c-e].

Bottom Line: The effects on hyperalgesia and locomotors activity were also quantified.On the complete Freund's adjuvant-induced rheumatoid arthritis, VPME showed a significant protective effect with 8.12% inflammation against 25.00% for the control group after 2 days of the treatment.The extract (75 and 150 mg/kg) significantly reduced the score of arthritis with a maximum obtained on day 19(th) of the experimentation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Sciences, University of Maroua, P.O Box 814, Cameroon.

ABSTRACT

Background: Vitellaria paradoxa is a traditional medicinal plant of Cameroon. Several studies on this plant have focused on the cosmetic profile of its fruits. The present study focuses on the anti-inflammatory potency of stem barks extract of this plant.

Objective: The objective was to evaluate the effect of methanolic extract of V. paradoxa (VPME) stem barks on inflammatory response in rats.

Materials and methods: Anti-inflammatory effects of VPME were evaluated in acute and chronic (28 days) inflammation induced in Wistar albino rats. The effects on hyperalgesia and locomotors activity were also quantified. The relative weight of lymphoid organs was obtained as well as some hematological parameters.

Results: In the carrageenan-induced inflammation, VPME (75 mg/kg) exhibited a significant (66.67%) inhibition after 1 h. On the complete Freund's adjuvant-induced rheumatoid arthritis, VPME showed a significant protective effect with 8.12% inflammation against 25.00% for the control group after 2 days of the treatment. The extract (75 and 150 mg/kg) significantly reduced the score of arthritis with a maximum obtained on day 19(th) of the experimentation. There was a significant increase in the reaction time of rats on the hot plate as well as the exploratory activities of the animals in the open field. This extract significantly prevented weight, hemoglobin and red blood cells losses, and spleen hypertrophy. A protective action against skin destruction and cartilage erosion was evident. Liquid chromatography-mass spectrometry analysis of the extract revealed the presence of catechins.

Conclusions: These findings suggested that V. paradoxa may contribute to the reduction of the inflammatory response.

No MeSH data available.


Related in: MedlinePlus